0S64
Poster Presentation - Gastro-intestinal Infections
AFP was observed in cytoplasm of Bel 7402 cells; The results is also showed that FRET was generated between FITC and TRITC which labeled PTEN and AFP respectively, and appeared the distance of fluorescent molecules was 6.7±1.5 Â; Treated with ATRA could enhance the expression of PTEN and decrease the phosphorylation of AKT after knockdown the expression of AFP.
Conclusions: These data provide the first evidence that AFP has a property to interact with PTEN and inhibit the activity of PTEN, this is also the pivotal events that AFP activated the transduction of PI3K/AKT signal of hepatoma cells.
PP-051 Clinical analysis of chronic liver failure and
decompensated cirrhosis in patients with blood gas analysis
Chen Li*-1-2, Huifen Wang1, Weiwei Yan3. 1The Department of Liver Failure Treatment and Research Centre, The 302ed Hospital of PLA, Beijing,China; 2The Second Military Medical University, Shanghai, China; 3The Department of Liver Disease of Traditional Chinese and Western Medicine,The 302ed Hospital of PLA, Beijing, China
Objective: To investigate clinical significance and pathogenesis of Change of blood gas analysis on Chronic liver failure and decompensated cirrhosis.
Methods: The blood gas analysis datas of 37 cases of chronic liver failure and decompensated cirrhosis were analyzed retrospectively
Results: 36 cases of patients with acid-base imbalance, alkalosis-based type of acid-base imbalance is common, respiratory alkalosis, respiratory alkalosis combined metabolic acidosis and respiratory alkalosis combined metabolic alkalosis have a higher proportion,the two groups of various types of acid-base imbalance in blood gas analysis indexes have no significant (P>0.05), 10 cases of patients with hypoxemia.
Conclusion: Chronic liver failure and cirrhosis patients affect acid-base imbalance of the body and the existence of hypoxemia, and have the similar Mechanism. Dynamic monitoring of blood gas analysis and timely symptomatic treatment of such patients have a clinical significance.
mRNA local expression in the pulmonary tissue, but it did not dramatically increase and/or decrease the serum level of IFN-y. Moreover, Th1 cytokines (IL-12 and/or IL-2) were observed to strongly correlate with the inhibition of pathological colonies. Conclusion: The accumulation of inflammatory mononuclear leucocytes at sites of infection is considered to be regulated by the local production and secretion of IFN-y, and the sera levels of IFN-y did not be used as potential indicator for therapeutic monitoring.
PP-053 Cryptococcal meningitis in HIV-negative patients
Yuanjie Zhu*, Hai Wen. Mycology Center & Department of Dermatology, Changzheng Hospital, SMMU
Objective: To describe the clinical characteristics, mycological profile, treatment, and outcomes of cryptococcal meningitis in HIV-negative patients.
Methods: HIV-negative adult patients with positive cerebrospinal fluid culture for Cryptococcus neoformans who attended Changzheng Hospital between 1997 and 2008 were retrospectively reviewed.
Result: During the 12 year review period, 62 HIV-negative patients with cryptococcal meningitis were identified. Thirty-four patients (55%) had associated underlying conditions and twenty had bird-droppings contact history. The most common associated conditions included immunosuppressive drug treatment, connective tissue disease, and diabetes mellitus. Intravenous Amphotericin B and oral 5 flucytocin for at least 6 weeks with continued oral fluconazole or itraconazole for at least 10 weeks showed to be effective treatment choice. During the treatment, mycological test result showed that CSF culture result turned to be negative in all patients after 2 weeks of treatment, and CSF antigen test showed 100% positive rate and decreased slowly and unregularly during and after treatment. The overall mortality rate was very low.
Conclusion: Cryptococcal meningitis is not rare in HIV-negative patients. Antifungal therapy with comparatively long periods in these patients may improve clinical outcomes, which was confirmed by mortality rate and mycological test result.
Poster Presentation - Fungal Infections
Poster Presentation - Gastro-intestinal Infections
PP-052 The inducible efficacy of IFN-y provoked by
interleukin-12 and/or low dose interleukin-2 in invasive pulmonary aspergillosis (IPA) mice model
Changran Zhang*, XiaoYun Liu, JianCong Ling, Ming Li, QinHua Tan, MiaoLian Tan. Department of Internal Medicine, Huang Pu Hospital of the First Affiliated Hospital, Sun Yat-Sen University
Background: Invasive Pulmonary Aspergillosis (IPA) is an intracellular opportunistic fungus causing invasive pulmonary mycosis, characterized by hyphal invasion and destruction of pulmonary tissue. Little is known about the expression of IFN-y in the serum and pulmonary tissue in IPA infection, which induced by the combination of IL-12 and IL-2 in IPA infection. The inducible efficacy of IFN-y and inhibition effect for Aspergillus fumigatus infection in the pulmonary by IL-12 and/or IL -2 was assessed in the study Methods: The animal model of pulmonary aspergillosis infection was prepared; Real-time RT-PCR assays for specifically quantify mouse IFN-y transcripts, and an assay for Lung CFU (Colony-forming unit). Histopathological sections were observed in the different interleukin treatment groups respectively. Results: Our results showed that the inhibition of A. fumiga-tus from lungs of immunosuppressed mice is correlated with Th1 cytokines, Interleukin-12 was proven to enhance the IFN-y
PP-054 Helicobacter pylori infection in patients with digestive complaints in northeastern Iran
Mahboobeh Nakhaei Moghaddam ^, Mahdi
Nakhaei Moghaddam 2. 11slamic Azad University, Mashhad
Branch, Iran; 2Hamun Medical Clinic, Mashhad, Iran
Background: Helicobacter pylori (HP) infection is the most common gastrointestinal bacterial disease worldwide. Although culture method is golden standard method for diagnosis, urea broth test (UBT) is non-invasive method with high specificity and sensitivity, relatively. The aim of this study is non-invasive detection of H. pylori infection prevalence in patients with digestive discomforts using UBT in Mashhad located in Iran northeastern. Methods: The study involved 814 patients (467 women and 347 men, 17-80 years) from January 2007 to November 2008, who had symptoms. The 14C-UBT was performed by ingestion of a solution of labeled urea by patient. The labeled CO2 is absorbed by the blood and exhaled in expired air. The expired air was collected by the Heliprobe breath card (a color change indicated that a sufficient volume of CO2 had been collected) and analyzed (Noster System, Stockholm, Sweden).
Results: 698 (85.75%) of 814 studied patients were positive and 116 14.25%) were negative. 403 (86.30%) of women and 295 (85.01%) of men had HP and no significant difference was observed between two groups (p>0.05). Positive cases were clas-
Poster Presentations
sified according age. The highest rate of infection was observed in 50-60 years old patients.
Conclusion: Since HP is related to dangerous disease, therefore diagnosis and treatment of infected people's especially symptomatic cases is significant. UBT make possible more recognition of infected people (in previous study the rate of infected people was 62.56% in patients with digestive complaints by direct stain, urease and culture method).
PP-055 Detecting the genotypes of Helicobacter pylori in the development of gastric carcinoma subjects in Andhra Pradesh population
G. Manoj*, Tiwari Santosh, G. Sivaram, R. Saikant, Bardia Avinash, HabeebAejaz, Khan Aleem, C.M. Habibullah. Center for Liver Research and Diagnostics
Background and Aim: Helicobacter pylori has been a key determinant causing gastric adenocarcinoma. Not much is known of the genotypes of H. pylori infection among the gastric cancer subjects. Therefore the present study was designed to ascertain the genotypes of H. pylori in gastric cancer subjects. Methods: 72 patients grouped on their endoscopic findings. Gastric biopsies were obtained for culture and DNA isolation. Results: Genotypic analysis showed cagT+ve/cagA+ve/cagE+ve/ vacAs1+ve genotype to be highly prevalent in 81.2% cases. This genotype was predominant among the Group-I subject those with gastric adenocarcinoma. Intestinal type adenocarcinoma subjects 91.4% harbored the remaining genotypes. Conclusion: Certain genotypes of H. pylori in this study had higher predictive values for the development of intestinal type carcinoma at an early age. Further this study also showed geno-typing H. pylori could well be used as an ideal tool for screening subjects at an increased risk of developing malignancy.
PP-056 Status of hepatitis A surveillance in China
Fuqiang Cui *. Chinese Center for Disease Control and Prevention
Background: To better define the current epidemiology of hepatitis A and status of surveillance in China, in anticipation of introduction of universal hepA vaccination of young children in 2008.
Methods: Data from national notifiable disease reporting system from 1990 to 2007 was reviewed and epidemiology characteristics analyzed. Hepatitis A vaccine distribution was also reviewed. Result: Incidence of hepatitis A has declined by 90% since 1990; declines in age specific incidences were seen in all age groups, but most dramatically among children less than 10 years. Disease incidence still varies substantially, with poorer Western provinces having highest incidence since 2000. In the high incidence provinces, young children less than 10 years continue to have high disease incidence. Over 135 million doses of hepatitis A vaccine have been distributed since 1992, with increasing use since 2003.
Conclusion: Although incidence of hepatitis A has decreased in all age groups, high risks remain among Western populations with transmission predominantly among children. The epidemiology of hepA transmission is not well defined, and only 50% of cases are laboratory confirmed. Improved surveillance with more laboratory confirmation is needed to monitor impact of universal hepatitis A vaccination.
PP-057 Risk of glandular atrophy, intestinal metaplasia and dysplasia in subjects with vacA positive and complete or disrupted cagE, cagT Helicobacter pylori infection
Tiwari Santosh*-1-2, G. Manoj1,2, G. Sivaram1-2, R. Saikant1,2, Bardia Avinash1-2, HabeebAejaz1,2, Khan Aleem1,2, C.M. Habibullah1,2. 1 Deccan College of Medical Sciences; 2Center for Liver Research and Diagnostics
Introduction: Glandular atrophy, Intestinal metaplasia and dysplasia are pre-neoplastic lesions common in patients with chronic gastritis as in those with Hp infection. Therefore the present study was to investigate the association among Hp infection with cagE, cagT and vacA genes and the risk of precancerous lesions. Methods: 120 patients were divided into different groups. Results: Genotypic data revealed 42.5% strains with cagE+/ cagT+/vacAs1+, and 7.5% strains with partially deleted cagE, cagT & vacAs2. With respect to the clinical status, 67.5% subjects with overt diseases were infected with cagE+/cagT+/vacAs1 + genotype whereas 22.5% NUD subjects harbored strains with partial deletions and s2 allele of vacA (p<0.01). Histology revealed atrophy, IM and dysplasia to be more prominent among subjects harboring above genotype compared to those with partially deleted genes (p<0.05).
Conclusion: Results demonstrates that the risk of overt gastric diseases was progressively higher as the number of virulence genes possessed by Hp increased.
PP-058 Helicobacter pylori genotypes in different ethnic groups resident in Tehran, Iran
Hossein Dabiri*,1,2, Akbar Mirsalehian1, Yoshio Yamaoka3, Fereshteh Jafari2, Kaveh Baghai2, Leila Shokrzadeh2, Maryam Rezadehbashi2, Mahsa Molaei2, Homayoun Zojaji2, Mohammad RezaZali2. 1 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; 2Research Center for Gastroenterology and Liver Diseases in Shaheed Beheshti University, M.C., Tehran, Iran; 3Department of Medicine-Gastroenterology, Michael E. DeBakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas, USA
Objectives: There is a geographic variation in Helicobacter pylori genotypes. cagA and cagE, oipA and vacA genotypes of H. pylori are associated with peptic ulcer disease (PUD). This study compared the distribution of these genotypes in major ethnic groups residing in Tehran, Iran and their association with clinical outcomes.
Methods: H. pylori infected patients proven by culture were recruited prospectively. DNA was extracted from isolated H. pylori and PCR was carried out to determine the cagA, cagE and oipA status and vacA alleles.
Results: A total of 124 patients living in Tehran were enrolled in this study. The ethnic distribution was 74 Persian, 33 Turkish and other ethnics including 7 Kurdish, 5 Lurs, 3 Afghani and 2 Arab patients. The predominant vacA signal region genotype was s1 among isolates from all ethnics. The vacA middle region genotypem2 was predominant in Persian and Turks. Of the Persian, Turkish and other ethnic isolates, 64.9%, 72.7% and 70.5%, respectively, were cagA positive, and 47%, 30% and 76.5%, respectively, were cagE positive. The oipA gene was present in 51.4% of Persian, 33.3% of Turks and 70.5 of others ethnics isolates.
Conclusion: There is difference in the H. pylori strains among the ethnic groups in Iran. However, there was no significant association between cagA, cagE and oipA status or vacA genotypes and clinical outcomes in Iranian patients irrespective of ethnic groups. None of these markers were helpful in predicting the clinical presentation of a H. pylori infection in Iran.
