Scholarly article on topic 'OL-036 Oxidative stress, immune dysfunction and inflammatory responses in benzene workers'

OL-036 Oxidative stress, immune dysfunction and inflammatory responses in benzene workers Academic research paper on "Clinical medicine"

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Academic research paper on topic "OL-036 Oxidative stress, immune dysfunction and inflammatory responses in benzene workers"

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agglutination (TPPA) of cerebrospinal fluid (CSF) help to diagnose. These cases recovered after 3 weeks' therapy with penicillin. Myocardial damages and hepatic injuries were observed in 3 cases complicating cytomegalovirus (CMV) infection, and ganciclovir therapy is responsive. 1 case with mass proteinuria and edema didn't undergo kidney biopsy. In conclusion, the initial symptoms of congenital syphilis are always single and unspecific, however, the manifestations are various with multi-organ injury, and laboratory findings help to diagnose. As an effective means of therapy, penicillin is of choice.

|QL-034| Research of T-cell subsets of

hand-foot-and-mouth disease (HFMD) H.H. Zeng*, Y. Gu, Z.H. Chen. Infection Department of Beijing Ditan Hospital, Beijing, 100015, China Objective: To evaluate the dynamic changes of T-Cell Subsets in children with hand, foot and mouth disease to provide new evidence for the therapy and prognosis. Methods: Two peripheral venous blood samples of 76 HFMD cases in acute stage and in recovery time of HFMD were collected. T-Cell Subsets were assayed by flow cytometer. Results: (1) The dynamic changes of T-Cell Subsets: the Th and T lymphocytes/lymphocytes of all cases step-up in recovery time of diseases (p < 0.05). General cases had the same pattern, every index of severe case have increased apparently (p <0.05). (2) The differentiation of T-lymphocyte subsets of general cases and severe cases: Th and T-lymphocytes and lymphocytes of pathoformic severe cases is lower than which in general cases (p < 0.05). In recovery time, there is no differentiation of T-Cell Subsets in severe cases and general cases (P >0.05). (3) The relationships of T-Cell Subsets and Length of Stay: Th/lymphocyte is correlated to Length of Stay in some degree.

Conclusions: The insufficient cellular immune function is the reason of childrens affectability to HFMD, the magnify cellular immune function and state of an illness is at equal pace; T-Cell Subsets can warn the course of disease in some degree.

|QL-035| Assessing health-related quality of life of liver transplantation recipients

Y. Liu*, L. Jiang, Y. Mu. The surgery department of Beijing Ditan Hospital, Beijing, China

Objective: To assess pretransplantation and posttransplantation health-related quality of life (HRQL) of liver transplant recipients.

Methods: HRQL was assessed at several timepoints using the Medical Outcomes Short Form 36 (SF-36). During January 2004 to December 2006, 34 individuals selected to receive treatment at the surgery department of Beijing Ditan hospital in China were eligible to be included. Results: A paired comparison of HRQL at listing and 3 months posttransplantation showed statistically significant improvements (P < 0.05) in all dimensions of the SF-36, except for Bodily Pain [P>0.05]. An analysis of posttransplantation HRQL over time for patients who survived until 24 months posttransplantation showed a statistically significant improvement (P < 0.05) for all dimensions of the SF-36 (apart from Mental Health [P = 0.083 > 0.05] and Rol^Emotional dimensions[P = 0.105 >0.05]). Several dimensions of the SF-36 were found to be associated with a number of pre-transplant factors: age, disease severity (classified by Child Pugh class), and liver transplant history (whether the patient had received a single or multiple transplants). Conclusion: Liver transplantation could significantly improve quality of life (QOL) of liver transplant recipients. The model for end-stage liver disease (MELD) score was not

predictive of QOL. In making clinical decisions about the use of transplantation, consideration should be given to the key factors likely to affect subsequent health-related quality of life.

Table 1. SF-36: paired comparison transplantation patient profiles

of listing and 3-month post-

Dimensions Mean score (n = 34) t-Statistica P

Listing 3 months post-Tx

PF 39.12±19.09 51.76±9.12 -3.413 0.001

RP 0.02±19.35 20.00±10.15 -4.718 <0.001

BP 63.24±22.01 56.76±10.07 -1.391 0.164

GH 25.59±18.82 59.85±10.19 -6.240 <0.001

VT 39.12±26.53 54.56±11.77 -3.225 0.001

SF 38.75±24.07 60.59±10.99 —4.340 <0.001

RE 32.16±35.02 57.97±12.37 —4.015 <0.001

MH 52.26±18.67 66.32±9.32 -3.765 <0.001

at-test; P< 0.05.

Table 2. SF-36: paired comparison of 3-, transplantation patient profiles

6-, 12- and 24-month post-

Dimensions Post-Tx mean score (n = 34) c2a P

3 months 6 months 12 months 24 months

PF 51.76 59.59 78.68 79.12 59.420 <0.001

±9.12 ±7.40 ±17.68 ±24.91

RP 20.00 30.71 78.97 78.82 80.849 <0.001

±10.15 ±10.97 ±21.03 ±27.00

BP 56.76 62.35 78.75 77.43 38.273 <0.001

±10.07 ±11.23 ±19.18 ±25.18

GH 59.85 61.03 71.32 62.21 8.300 0.040

±10.19 ±10.28 ±25.71 ±25.94

VT 54.56 58.24 74.85 69.41 33.490 <0.001

±11.77 ±10.72 ±21.30 ±24.27

SF 60.59 69.12 75.66 75.59 28.236 <0.001

±10.99 ±9.25 ±23.86 ±29.21

RE 57.97 57.82 61.67 64.56 6.143 0.105

±12.37 ±12.19 ±30.08 ±39.62

MH 66.32 66.32 72.18 67.38 6.684 0.083

±9.32 ±9.32 ±22.66 ±25.48

aChi-square test; P< 0.05.

|QL-036| Oxidative stress, immune dysfunction and inflammatory responses in benzene workers

B. Santhosh Kumar1*, S.K. Tiwari2, G. Manoj2, U. Nazia1, R. Devender Reddy1. 1 Department of Physiology, Deccan College of Medical Sciences, Hyderabad-500 058, India,

2Center for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad-500 058, India Background and Aim: Benzene and its derivatives form active ingredients of petroleum solvents. Continuous exposure to these toxic metabolites is known to be hazardous as they drastically cause cytotoxicity, immunotoxicity, DNA damage and carcinogenesis. Professional petrol filling workers are at an increased risk to suffer with multitude of diseases. The present study therefore evaluated the oxidative stress and immune dysfunction among professional petrol filling workers continuously exposed to benzene and its derivatives. Methods: A total of 78 subjects (48 professional workers and 30 healthy volunteers) were recruited. Oxidative stress markers namely ROS, lipid peroxides, detoxifying agents such as glutathione and antioxidants were measured in blood samples collected from these subjects. Expression of p53 and COX-2 was also evaluated using reverse transcriptase polymerase chain reaction. The levels of immune cells like CD4+, and antibodies like IgG1 and IgG2 were measured to evaluate the immune status.

Results: Levels of ROS and lipid peroxides were elevated among the professional workers than the controls indicating

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increased oxidative stress. Expression of p53 and COX-2 showed transient elevation during the initial period of working but gradually declined as a result of acclimatization to benzene and other solvents. The levels of detoxifying agents like glutathione, superoxide dismutase, glutathione peroxidase were found to be decreased. It was also noticed that the levels of IgGs and immune cells like CD4+ were decreased.

Conclusion: The study shows that benzene and its metabolites significantly increased oxidative stress and immune parameters in petrol filling workers thereby making them more susceptible to diseases compared to the others with minimal exposure.

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|OL-Q37| Impact of malnutrition in survival of

HIV-infected children after initiation of antiretroviral treatment (ART)

B. Taye*, S. Shiferaw, F. Enqusilasie. School of public health, Addis Ababa University, Ethiopia

Background: Malnutrition is a common condition in HIV-infected children; however, its impact in survival of HIV infected children after initiation of antiretroviral therapy is not well understood.

Objective: To assess the impact of malnutrition in survival of HIV infected children after initiation of antiretroviral treatment.

Methods: A retrospective cohort study was conducted in HIV infected children starting antiretroviral treatment at Zewditu memorial hospital, Addis Ababa, Ethiopia. Demographic, nutritional, clinical and immunological data were carefully extracted from the existing ART logbook and patient follow up cards. Nutritional status were defined with stunting (height for age Z score <-2), Wasting (weight for height Z score -2) and under weight (weight for age Z score <-2). Survival was defined as the time from nutritional and immunologic evaluation to death. Data were analyzed for uinvariate and multivariate analysis using Cox regression proportional hazard model. Survival rate was calculated and compare with the Kaplan Meier and log rank tests. Results: A total of 475 HIV infected children starting antiretroviral treatment (ART) from March 21 2005 to 30 April 2008 were included in the study. Of whom 42 (8.8%) died during a median study follow up of 12 months. Independent baseline predictors of mortality were severe wasting (Hazard ratio (HR) = 4.99, 95% CI 2.4-10.2, P < 0.00), absolute CD4 below the threshold for severe immunodeficiency (HR = 3.02, 95% CI 1.02-8.96, P = 0.04) and low hemoglobin value (HR = 2.92, 95% CI 1.3-6.7, P = 0.001 for those hemoglobin value <7.0gm/dl). Conclusion: Despite the apparent benefit of ART use on HIV related survival, severe wasting (WHZ <-3) appear to be strong independent predictor of survival in HIV infected children receiving ART.

|OL-Q38| Preparation of a human full-IgG antibody against CCR5 coreceptor

S.J. Wei1*, X. Liou2, A. Marasco2, J. Sodroski2. 11nfectious Disease Institute of Guangzhou the 8th People Hospital, Guangzhou, 510050, China, 2Dana-Farber Cancer Institute, Harvard Medical School, Boston, USA

CCR5 was identified as a key coreceptor for HIV-1 entry. A deletion of 32 base pairs was found in the CCR5 gene

open reading frame in a few persons who were at high risk for HIV entry but who had remained free of infection. Thus, inhibition of CCR5 protects against acquisition of HIV infection. However, all previously reported anti-CCR5 gene antibodies were not humanized and not suitable for human application. In this study, One of our selected single chain antibodies (scFvs) to CCR5, termed A8, was mapped to the residues 9-13 of hCCR5 N-terminal (Nt) by using CCR5 mutagenesis and flow cytometric analysis. A8 binding on CCR5 did not affect chemokiine-CCR5 activities and effectively blocked HIV-1 entry in vitro. A8 was then successfully converted into human IgG by cloned into an expressing vector TCAE 6. The role of human A8 IgG for inhibition of HIV entry was further characterized. The result revealed that A8 function of HIV-1 entry inhibition might be mediated by the blockage of a unique and a great conformational-dependent epitope of CCR5 Nt. The recognition of the epitope rendered A8 a higher affinity binding on a great proportion of cell surface CCR5 molecules when compared that of previously reported antibodies. Our study showed that human A8 IgG is functional antibody against CCR5 and is a great potential candidate for antibody therapy of human HIV infection in vivo. This study was granted by research foundation from NIH of USA and from The Board of Health of Guangzhou

|OL-Q39| The theory study of "All-round responsibilities system" for the control emergency public health disasters X.D. Tan1*, D.J. Zhou2, Q. Song1, L. Wang2, J.G. Xie1, D. Zhou1. 1 School of Public Health, Wuhan University, China, 2Center of Diseases Control and Prevention, Wuhan, China

Background: A new method, called "All-round responsibilities system" was firstly introduced into the control of "hand-foot-mouth" disease in 2008, but a lot of unsolved issues were found.

Objectives: This article presented firstly the detail methodology of the "All round responsibilities system". Methods: By use the literature analysis, field investigation and experts deeping talking methods, extensive issues were analyzed, and key issues of the methodology was described.

Results: "All round responsibilities system" was original from England more than 100 years ago, It was firstly used in control of emergency public health disaster in 2008. The key issues of the methodology were guarantee system which included financial, human sources, and ascertain where the responsibility lies.

Conclusions: The definition of the "All round responsibilities system" was given, and technological contents of the methodology were performed, and it could be used well for the future controlling of emergency public health disaster.

|OL-04Q| Sarcoidosis in an HIV-positive patient presenting as a mediastinal mass

P. Papineni1 *, G.D. Thomas1, T.J. McManus2, T. O'Shaughnessy1. 1 Department of Respiratory Medicine, Newham University Hospital, London, UK, 2The Greenway Centre, Newham University Hospital, London, UK

Introduction: The immunological basis of sarcoidosis involves CD4 T lymphocytes for granuloma formation. Lack of CD4 cells with HIV infection has meant the association of HIV and sarcoidosis has rarely been described. Establishment of highly active anti-retroviral therapy causing immune restoration however, can result in concomitant sarcoidosis and HIV infection.