Scholarly article on topic 'Identification of Drought Tolerance Candidate Proteins and Genes in Reproductive Stage of Wheat'

Identification of Drought Tolerance Candidate Proteins and Genes in Reproductive Stage of Wheat Academic research paper on "Agriculture, forestry, and fisheries"

CC BY-NC-ND
0
0
Share paper
Academic journal
Procedia Environmental Sciences
OECD Field of science
Keywords
{"Drought stress" / "anther proteome" / "reproductive phase" / "meiosis stage" / "wheat pollen grain"}

Abstract of research paper on Agriculture, forestry, and fisheries, author of scientific article — Reza Fotovat

Abstract Reproductive development is the most water-stress-sensitive phase in the life of a cereal crop. We applied a proteomic approach to analysis the effects of drought stress on anther proteome and identification of candidate proteins and genes in drought tolerant, Dezful-10 and susceptible, Shiraz, wheat genotypes. Our results showed that short-term stresses at meiosis and mitosis stages declined plant seed setting and the meiosis stage is more sensitive to drought stress. Mass spectrometry analysis of responsive proteins revealed that several important protein in plant adaptation to drought stress are up-regulated only in tolerant plant including raftin, vacuolar invertase, putative PrMC3, Esterase/lipase, reversibly glycosylated polypeptide and subtilisin like serine protease. The expression of the selected proteins which were the representatives of the down-regulated proteins and of the up-regulated proteins in wheat genotypes were further assessed at the transcription level using real-time PCR.

Academic research paper on topic "Identification of Drought Tolerance Candidate Proteins and Genes in Reproductive Stage of Wheat"

CrossMark

Available online at www.sciencedirect.com

ScienceDirect

Procedia Environmental Sciences 29 (2015) 272 - 273

Agriculture and Climate Change- Adapting Crops to Increased Uncertainty (AGRI 2015)

Identification of drought tolerance candidate proteins and genes in

reproductive stage of wheat

Reza Fotovata*

aDepartment of Agronomy and Plant Breeding, University of Zanjan, Zanjan, IRAN

Abstract

Reproductive development is the most water-stress-sensitive phase in the life of a cereal crop. We applied a proteomic approach to analysis the effects of drought stress on anther proteome and identification of candidate proteins and genes in drought tolerant, Dezful-10 and susceptible, Shiraz, wheat genotypes. Our results showed that short-term stresses at meiosis and mitosis stages declined plant seed setting and the meiosis stage is more sensitive to drought stress. Mass spectrometry analysis of responsive proteins revealed that several important protein in plant adaptation to drought stress are up-regulated only in tolerant plant including raftin, vacuolar invertase, putative PrMC3, Esterase/lipase, reversibly glycosylated polypeptide and subtilisin like serine protease. The expression of the selected proteins which were the representatives of the down-regulated proteins and of the up-regulated proteins in wheat genotypes were further assessed at the transcription level using real-time PCR.

* Corresponding author. Tel.: +98-912-141-4803; fax: +98-2432283202. E-mail address: r_fotovat@znu.ac.ir

© 2015Published byElsevierB.VThisis anopenaccess article under the CC BY-NC-ND license (http://creativecommons.Org/licenses/by-nc-nd/4.0/).

Peer-reviewunderresponsibilityoftheorganizing committee of the Agriculture and Climate Change - Adapting Crops to Increased Uncertainty(AGRI2015)

Keywords: Drought stress; anther proteome; reproductive phase; meiosis stage; wheat pollen grain

References

1. Barnabas B, Jager K, Feher A. The effect of drought and heat stress on reproductive processes in cereals. Plant, Cell and Environment 2008; 31(1):11-38.

1878-0296 © 2015 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Peer-review under responsibility of the organizing committee of the Agriculture and Climate Change - Adapting Crops to Increased

Uncertainty (AGRI 2015)

doi : 10. 1016/j .proenv.2015.07.206

Reza Fotovat / Procedía Environmental Sciences 29 (2015) 272 - 273

2. Ji X, Shiran B, Wan J, Lewis DC, Jenkins CLD, Condon AG, Richards RA, Dolferus R. Importance of pre-anthesis anther sink strength for maintenance of grain number during reproductive stage water stress in wheat. Plant Cell and Environment 2010;33(6):926-942.

3. Koonjul PK, Minhas JS, Nunes C, Sheoran IS, Saini HS. Selective transcriptional down-regulation of anther invertases precedes the failure of pollen development in water-stressed wheat. Journal of Experimental Botany 2005;56(409):179-190.