Scholarly article on topic 'Hypoxic preconditioning enhances mesenchymal stromal cell lung repair capacity'

Hypoxic preconditioning enhances mesenchymal stromal cell lung repair capacity Academic research paper on "Nano-technology"

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Stem Cell Research & Therapy
OECD Field of science

Academic research paper on topic "Hypoxic preconditioning enhances mesenchymal stromal cell lung repair capacity"

Cruz and Rocco Stem Cell Research & Therapy (2015) 6:130 DOI 10.1186/s13287-015-0120-3


Open Access

Hypoxic preconditioning enhances ®

mesenchymal stromal cell lung repair capacity


Fernanda Ferreira Cruz and Patricia Rieken Macedo Rocco

See related research by Lan et al., http://www.stemcellres.c0m/c0ntent/6/l/97


Idiopathic pulmonary fibrosis is a progressive, irreversible, debilitating, and fatal lung disease, characterized by parenchymal fibrosis with reduced lung volumes and respiratory failure. No lasting option for therapy is available other than transplantation. Mesenchymal stem/stromal cells home to sites of injury, decrease inflammation, have antifibrotic properties, and promote epithelial tissue repair, so their use has been suggested as potential therapy for idiopathic pulmonary fibrosis. Despite reported benefits, the amount of mesenchymal stromal cells engrafting to the lung decreases substantially soon after administration. New strategies, such as hypoxia preconditioning, have thus been investigated in an attempt to optimize the engraftment, survival, and paracrine properties of stem cells. Hypoxia induces the expression of prosurvival mediators, chemoattractants, and growth factors involved in cell proliferation, migration, angiogenesis, antioxidant, antiapoptotic, and antifibrotic properties in mesenchymal stromal cells, optimizing their lung repair capability in an animal model of idiopathic pulmonary fibrosis.


Idiopathic pulmonary fibrosis (IPF) is a chronic, diffuse, fibrotic disease of the lung parenchyma of unknown etiology. Progressive deposition of collagen fibers in the lung interstitium inevitably leads to fatigue, dyspnea, hypoxia, and respiratory failure in patients with endstage IPF. Although current pharmacological and non-pharmacological therapies can temporarily lessen the severity of symptoms, no effective treatment for IPF has been developed to rescue the impaired lung, prevent lesion progression, or both. Lung transplantation is considered the only curative approach. However, the significant shortage of suitable donor lungs and the many complications related to post-transplantation immuno-suppression mean there is a dire need for new therapeutic approaches. In this context, mesenchymal stem/ stromal cell (MSC)-based therapy is a promising alternative for the treatment of lung diseases. Novel strategies to optimize the beneficial effects of cell therapy have

* Correspondence:

Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, FederalUniversity of Rio de Janeiro, Centro de Ciencias da Saude, Avenida Carlos Chagas Filho, s/n, Bloco G-014, Ilha do Fundäo 21941-902, Rio de Janeiro, RJ, Brazil

Bio Med Central

been investigated, seeking to enhance the proliferation, survival, engraftment, and paracrine properties of MSCs.

In a recent issue of Stem Cell Research & Therapy, Lan et al. [1] demonstrated that transplantation of hypoxia-preconditioned MSCs exerted better therapeutic effects in a mouse model of bleomycin-induced pulmonary fibrosis and enhanced the survival rate of engrafted MSCs, partially due to upregulation of hep-atocyte growth factor (HGF).

Main text

Repair of fibrotic lung parenchyma remains a significant clinical challenge. All currently available therapeutic trials in IPF are severely limited due to the lack of a clear understanding of the natural history of the disease. To date, treatment of IPF has been based on the concept that inflammation leads to injury; thus, most therapies have been based on eliminating or suppressing the inflammatory component through administration of anti-inflammatory agents including corticosteroids, immunosuppressants/cytotoxic drugs (e.g., azathioprine, cyclophosphamide), and anti-fibrotic agents (e.g., colchi-cine or D-penicillamine), alone or in combination [2].

© 2015 Cruz and Rocco. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http:// applies to the data made available in this article, unless otherwise stated.

Cruz and Rocco Stem Cell Research & Therapy (2015) 6:130

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Despite this variety of pharmacological options, no drug-based therapy has been proven unequivocally to alter or reverse the fibrotic process.

MSC-based therapy is an attractive alternative approach for the treatment of IPF, as MSCs home to sites of injury, inhibit inflammation, and contribute to epithelial tissue repair [3]. In addition, MSCs secrete paracrine mediators with antiapoptotic, anti-inflammatory, and antifibrotic effects [3]. Alvarez et al. [4] showed that lung-engrafted MSCs possessed the ability to ameliorate fibrotic effects in mice challenged with bleomycin. However, despite reported successes, the amount of engrafted MSCs decreased dramatically 1 day after transplantation due to exposure to harsh, toxic, and oxidative microenvironments [3]. New strategies to optimize cell therapy outcomes are therefore being investigated. A recent study involving overexpression of antioxidants, chemokine receptors, antiapoptotic genes, prosurvival genes, or growth factors in engrafted stem cells has shown improved cell survival following transplantation [5]. Additionally, cells may be preconditioned by sublethal exposure to selected stressors to induce prior expression of cytoprotective genes before subsequent lethal challenges [6]. Cellular preconditioning may include exposure of cells to physiological stimuli such as heat shock, small-molecule pharmacological agents, cytokines, growth factors, biophysical stimuli, or hypoxia [7]. Choosing appropriate preconditioning strategies may provide a simple yet effective way of promoting survival, enhancing regenerative properties, and boosting the tissue repair capability of transplanted cells in stem cell-based therapy [8].

The main insight of Lan et al.'s study was the use of hypoxic preconditioning (HP), which upregulated cyto-protective and regenerative genes, stabilized mitochon-drial membrane potentials, increased homing ability, promoted cell proliferation, and acted against hydrogen peroxide-induced cell death in the treated MSCs. Moreover, hypoxia-preconditioned mesenchymal stem/stromal cells (HP-MSCs) attenuated bleomycin-induced cell apop-tosis and extracellular matrix (ECM) production through transforming growth factor (TGF)-pl-mediated Akt signaling via paracrine effects. Lan et al. were the first to show that engraftment of HP-MSCs had therapeutic effects superior to those of untreated MSCs in a model of bleomycin-induced pulmonary fibrosis. Engafted HP-MSCs improved lung function, reduced lung edema, and reduced levels of proinflammatory and fibrotic factors. HP and stem cell transplantation have been extensively studied in a number of organs and tissues as a means of enhancing therapeutic effects in diseases such as stroke, myocardial infarction, traumatic brain injury, diabetes mellitus, inflammatory bowel disease, and acute kidney and liver injuries [7-9]. However, there have been no

published studies on the use of HP-MSCs to treat pulmonary fibrosis.

Furthermore, Lan et al. demonstrated that transplantation of HP-MSCs attenuated ECM deposition, in a mechanism attributed to upregulation of HGF in the fi-brotic lung. In this process, HGF plays a key role in preventing fibrosis or scar formation after injury, by inhibiting TGF-p-mediated myofibroblast differentiation and ECM production. HGF also exerts multiple protective effects on injured tissues via mitogenic, antiapoptotic, anti-inflammatory, and antifibrogenic signaling [10].


Lan et al. [1] suggest that hypoxia-preconditioned MSC therapy enhanced the survival rate of engrafted MSCs, exerted superior therapeutic effects, and improved lung functions in bleomycin-induced pulmonary fibrosis in mice. Further studies should be conducted to ascertain whether HP-MSCs have similar beneficial effects in other models of fibrosis.


ECM: Extracellular matrix; HGF: Hepatocyte growth factor; HP: Hypoxic preconditioning; HP-MSC: Hypoxia-preconditioned mesenchymal stem/ stromal cell; IPF: Idiopathic pulmonary fibrosis; MSC: Mesenchymal stem/ stromal cell; TGF: Transforming growth factor.

Competing interests

The authors declare that they have no competing interests.

Published online: 14 July 2015


1. Lan YW, Choo KB, Chen CM, Hung TH, Chen YB, Hsieh CH, et al. Hypoxia-preconditioned mesenchymal stem cells attenuates bleomycin-induced pulmonary fibrosis. Stem Cell Res Ther. 2015;6:97.

2. Spagnolo P, Maher TM, Richeldi L. Idiopathic pulmonary fibrosis: recent advances on pharmacological therapy. Pharmacol Ther. 2015;152:18-27.

3. Toonkel RL, Hare JM, Matthay MA, Glassberg MK. Mesenchymal stem cells and idiopathic pulmonary fibrosis. Potential for clinical testing. Am J Respir Crit Care Med. 2013;188:133-40.

4. Älvarez D, Levine M, Rojas M. Regenerative medicine in the treatment of idiopathic pulmonary fibrosis: current position. Stem Cells Cloning. 2015;8:61-5.

5. Leblond AL, Naud P, Forest V, Gourden C, Sagan C, Romefort B, et al. Developing cell therapy techniques for respiratory disease: intratracheal delivery of genetically engineered stem cells in a murine model of airway injury. Hum Gene Ther. 2009;20:1329-43.

6. Ogle ME, Yu SP, Wei L. Primed for lethal battle: a step forward to enhance the efficacy and efficiency of stem cell transplantation therapy. J Thorac Cardiovasc Surg. 2009;138:527.

7. Noort WA, Feye D, Van Den Akker F, Stecher D, Chamuleau SA, Sluijter JP, et al. Mesenchymal stromal cells to treat cardiovascular disease: strategies to improve survival and therapeutic results. Panminerva Med. 2010;52:27-40.

8. Tsai CC, Yew TL, Yang DC, Huang WH, Hung SC. Benefits of hypoxic culture on bone marrow multipotent stromal cells. Am J Blood Res. 2012;2:148-59.

9. Muscari C, Giordano E, Bonafe F, Govoni M, Pasini A, Guarnieri C. Priming adult stem cells by hypoxic pretreatments for applications in regenerative medicine. J Biomed Sci. 2013;20:63.

10. Crestani B, Marchand-Adam S, Quesnel C, Plantier L, Borensztajn K, Marchal J, et al. Hepatocyte growth factor and lung fibrosis. Proc Am Thorac Soc. 2012;9:158-63.