Scholarly article on topic 'Unusual conservation of mitochondrial gene order in Crassostrea oysters: evidence for recent speciation in Asia'

Unusual conservation of mitochondrial gene order in Crassostrea oysters: evidence for recent speciation in Asia Academic research paper on "Biological sciences"

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Academic research paper on topic "Unusual conservation of mitochondrial gene order in Crassostrea oysters: evidence for recent speciation in Asia"

Evolutionary Biology


Unusual conservation of mitochondrial gene order in Crassostrea oysters: evidence for recent speciation in Asia

Jianfeng Ren1,4, Xiao Liu1, Feng Jiang2,5, Ximing Guo3*, Bin Liu1,2*


Background: Oysters are morphologically plastic and hence difficult subjects for taxonomic and evolutionary studies. It is long been suspected, based on the extraordinary species diversity observed, that Asia Pacific is the epicenter of oyster speciation. To understand the species diversity and its evolutionary history, we collected five Crassostrea species from Asia and sequenced their complete mitochondrial (mt) genomes in addition to two newly released Asian oysters (C. iredalei and Saccostrea mordax) for a comprehensive analysis. Results: The six Asian Crassostrea mt genomes ranged from 18,226 to 22,446 bp in size, and all coded for 39 genes (12 proteins, 2 rRNAs and 25 tRNAs) on the same strand. Their genomes contained a split of the rrni gene and duplication of trnM, trnK and trnQ genes. They shared the same gene order that differed from an Atlantic sister species by as many as nine tRNA changes (6 transpositions and 3 duplications) and even differed significantly from S. mordax in protein-coding genes. Phylogenetic analysis indicates that the six Asian Crassostrea species emerged between 3 and 43 Myr ago, while the Atlantic species evolved 83 Myr ago.

Conclusions: The complete conservation of gene order in the six Asian Crassostrea species over 43 Myr is highly unusual given the remarkable rate of rearrangements in their sister species and other bivalves. It provides strong evidence for the recent speciation of the six Crassostrea species in Asia. It further indicates that changes in mt gene order may not be strictly a function of time but subject to other constraints that are presently not well understood.


Mitochondrial (mt) DNA is widely used for phylogenetic analysis because of its unique architecture, inheritance and small size. Metazoan mtDNA is nearly always a circular molecule except for some cnidarians [1]. It contains the same 37 genes, specifying 13 proteins of the respiratory chain [cytochrome c oxidase subunits I-III (cox1-cox3), apocytochrome b (cob), ATP synthase subunits 6 and 8 (atp6 and atp8), and NADH dehydrogenase subunits 1-6 and 4L (nad.1-6, nad4L)], 2 ribosomal RNAs and 22 transfer RNAs. Although there are exceptions, most mtDNAs range in size from 14 to 17 kb.

* Correspondence:; 1Key Laboratory of ExperimentalMarine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 3Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, NJ 08349, USA Full list of author information is available at the end of the article

Bio Med Central

Typically, there are few intergenic nucleotides except for a single large non-coding region generally thought to contain elements that control the initiation of replication and transcription [2]. Size variation in mtDNA is usually due to the different length of the non-coding regions. Gene order is generally conserved in most metazoan taxa although some groups show considerable variation.

Variations in both mtDNA sequence and gene order have been used for phylogenetic analysis. Because mtDNA is fast evolving and nucleotide mutations may return to an early state, mtDNA sequences may not allow deep phylogenetic reconstruction. Gene order, on the other hand, has very small probability of back-mutation and may be particularly useful for high level phylogenetic analysis. Although the mechanism of mtDNA rearrangement is poorly understood, mt gene order has been increasingly used for phylogenetic studies [3-6].

© 2010 Ren et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

As the second species-rich phylum of the animal kingdom after Arthropoda, Mollusca exhibits tremendous variation in their mt genomes. Seven bivalve lineages (Mytilidae, Unionidae, Margaritiferidae, Hyriidae, Dona-cidae, Solenidae, and Veneridae) have been found to have an unusual mode of inheritance for mtDNA, termed doubly uniparental inheritance (DUI) [7]. Some pulmonate gastropods have unusual tRNAs lacking the T-stem or the D-stem, similar to nematode mt tRNAs [8]. The atp6 and atp8 genes are separated in the sca-phopods and two groups of gastropods (Patellogastro-poda and Heterobranchia). In addition, several mt genes are duplicated in cephalopods Watasenia scintillans and Todarodes pacificus [9]. Unlike the general conservation in gene order in most other metazoan groups, most molluscan mt genomes reported so far contain considerable rearrangements especially in Bivalvia and Scapho-poda [10]. At the same time, the phylogeny of molluscs is poorly studied. Phylogenetic relationships among major molluscan groups are not well understood. The species identity and classification of some most common molluscs remain questionable.

Oysters are bivalve molluscs widely distributed in world oceans. They are benthic, sessile filter-feeders with important roles in estuary ecology. Some species support major fishery and aquaculture industries worldwide. Despite the abundance, ecological and economic significance of oysters, we know little about their species diversity and evolutionary history. Classification of oysters remains a challenge partly due to the lack of well-defined morphological characters. Shell morphology, the main character used in oyster classification, is known to be plastic and subject to environmental variation. Much of the oyster classification to date is based on shell characteristics and has resulted in considerable errors and confusion. The difficulty of oyster classification is particularly pronounced in China and other parts of Asia where a large number of species are sympatric [11,12]. About thirty species have been recorded along the coast of China [13]. The presence of a large number of oyster species has led many to believe that Asia Pacific is the epicenter of oyster speciation, but the inability to reliably identifying them has hindered our understanding of oyster evolution. Among the extent oysters, five Crassostrea oysters, namely C. gigas, C. angulata, C. sikamea, C. hongkongensis and C. ariakensis are commonly found in China and other parts of Asia and yet, they have been difficult to identify by shell morphology alone. Recently, some confusion in the oyster identification have been resolved using DNA sequence data [12,14-20], and the five Crassostrea oysters can be reliably identified [21]. Still, we know little about the evolutionary history of the Crassostrea species and how they relate to each other and the other oysters. For example,

it is not clear whether C. angulata should be considered as an independent species and which species is the closest relative of the newly described C. hongkongensis. Most of the phylogenetic analyses so far are based on short DNA fragments, yielding variable results [12,16,22].

The ability to sequence and compare whole mt genomes provides a new impetus for phylogenetic analysis of oysters and other molluscs. Complete mt genome sequences have been obtained for two Crassostrea oysters from Asia (C. gigas and C. hongkongensis) and one species from the Atlantic (C. virginica) [23-25]. Comparative analysis shows that the two Asian species share the same gene order that is very different from the Atlantic species [24,25]. To determine if gene order is conserved in other Asian species and to understand the evolution of Crassostrea oysters, we sequenced the mt genomes of four Crassostrea oysters from Asia plus one that was previously published [25], obtained newly released mt genomes of two Asian oysters (C. iredalei and Saccostrea mordax), and compared them with other molluscan genomes. Here we report the first estimates of divergence time among Crassostrea species based on complete mt sequences and the complete conservation in gene order among the six Asian Crassostrea species. The complete conservation of gene order is highly unusual considering the tremendous rearrangement of mtDNA in most marine bivalves. It provides strong evidence for recent emergence of the six Crassostrea species in Asia. Our analysis also suggests that rearrangement of the mt genome may not be strictly a function of time but subject to some other constraints that are presently not well understood.

Results and Discussion

Genome composition

Genome composition and organization of the six Asian Crassostrea oysters is summarized in Figure 1 and additional file 1: Table S1. Organization of the American oyster C. virginica, a sister species from the Atlantic Ocean, is listed for comparative analysis (additional file 1: Table S1). The complete mt genomes of C. gigas, C. angulata, C. sikamea, C. hongkongensis and C. aria-kensis are 18,225 bp, 18,225 bp, 18,243 bp, 18,622 bp and 18,414 bp in length, respectively [24,25]. These sequences have been deposited in GenBank under the accession number EU672831, NC_012648-NC_012650, NC_011518. The American oyster C. virginica mtDNA is 17,244 bp in length. The mt genome of S. mordax is 16,532 bp in length. However, the mt genome of C. ire-dalei, 22,446 bp in length, is obviously longer than all other oysters [54]. The size of molluscan mt genomes varies dramatically, ranging from 13,670 bp in the snail Biomphalaria glabrata to 40,725 bp in the sea scallop

Figure 1 Mitochondrial genome map of Asian Crassostrea oysters. All 39 genes are coded on the same DNA strand. Genes for proteins and rRNAs are shown with standard abbreviations. Genes for tRNAs are designated by a single letter for the corresponding amino acid with two leucine tRNAs and two serine tRNAs differentiated by numeral. The two tRNA duplications of methionine, lysine and glutamine are named trnMI, trnM2, trnKI, trnK2, trnQ1 and trnQ2, respectively. The length of five mt genomes is indicated at the lower right-hand corner.

Placopecten magellanicus [41,42]. The six Asian Crassostrea oyster genomes encode 39 genes including 12 protein-coding genes, 2 rRNAs and 25 tRNAs on the same strand (Figure 1). In contrast to the typical animal mt genomes, they lack one protein-coding gene atp8 and have duplications of three tRNAs: trnM, trnK and trnQ. Another unique character is that rrnL gene is split into two segments (also in C. virginica and S. mordax) and rrnS has a nearly identical duplication, which has never been reported in other animal mt genomes [23]. Among the seven Crassostrea oysters, the lowest A+T content is 61.1% in C. virginica, while the highest A+T content is 65.3% in C. hongkongensis. In the other five species, the A+T content varies from 62.9% in C. ariakensis to 64.5% in C. iredalei [54] (Table 1). The highest A+T

content of C. hongkongensis is mainly caused by the increased total length and high A+T content of the non-coding region. The length of coding regions in the Asian-Pacific oyster genomes is similar and larger than that in C. virginica, because of duplications of two tRNAs (trnK and trnQ) and one rrnS.

Gene arrangement

Molluscs, especially bivalves, display an extraordinary amount of variation in gene arrangement. Gene order of selected molluscs was presented and compared with that of oysters in Figure 2. The black chiton Katharina tunicata is the only sequenced representative of the Polyplacophora, an early diverged class of the Mollusca [43]. Its gene order may represent the pleisomorphic

Table 1 Genomic characteristics of bivalve mtDNAs

Species GenBank Accession Number Positive strand Protein-coding gene rrnL gene rrnS gene tRNA Largest NCR Reference

Length bp A+T % No. of AA A+T% Length bp A+T % Length bp A+T % Length bp A+T % Length bp A+T %

Total 1st cdn pos. 2nd cdn pos. 3rd cdn pos.

Lampsilis ornata NC_005335 16,060 62.4 3,710 61.6 56.5 61.6 67.1 1,315 62.8 846 60.5 1,368 64.6 283 64.7 [10]

Inversidens japanensis AB055625 16,826 57.2 3,647 56.6 53.6 59.4 56.9 1,304 58.3 845 56.6 1,416 60.5 1,196 57.5 Okazaki et al, unpublished

Hiatella arctica NC_008451 18,244 66.4 3,968 65.8 61.8 63.4 72.2 1,447 67.0 901 63.0 1,472 67.0 614 66.1 [48]

Acanthocardia tuberculata NC_008452 16,104 59.9 3,636 59.4 55.8 61.0 61.4 1,213 65.2 824 59.5 1,489 57.8 1,103 59.1 [48]

Venerupis philippinarum NC_003354 22,676 69.7 4,211 68.5 62.2 63.0 68.1 1,408 72.8 1,249 70.6 1,459 69.2 2,183 68.2 Okazaki et al, unpublished

Meretrix petechialis NC_012767 19,567 68.3 4,014 66.9 60.5 64.4 75.6 1,581 71.0 1,187 69.4 1,458 68.4 1,634 69.4 [50]

Mytilus galloprovincialis NC_006886 16,744 61.8 3,733 60.5 56.0 62.1 63.3 1,244 65.6 947 64.2 1,517 66.7 1,157 60.2 [46]

Mytilus edulis NC_006161 16,740 61.8 3,681 60.5 56.0 62.0 63.6 1,244 65.3 945 64.1 1,517 66.6 1,158 61.0 [51]

Mytilus trossulus NC_007687 18,652 61.5 3,716 59.5 54.9 62.1 61.6 1,244 66.8 948 63.6 1,584 67.1 1,561 63.9 [47]

Placopecten magellanicus NC_007234 32,115 55.7 3,742 55.7 54.3 58.1 54.6 1,387 58.0 970 52.9 2,294 50.1 3,112 60.4 [42]

Mizuhopecten yessoensis NC_009081 20,414 55.2 3,763 55.5 53.3 58.2 54.9 1,424 57.8 961 50.6 1,079 49.4 1,528 59.9 Sato et al, unpublished

Argopecten irradians DQ665851 16,211 57.3 3,681 57.0 52.7 58.5 59.6 1,292 59.5 904 56.8 1,365 52.5 1,038 63.6 [52]

Chlamys farreri EF473269 20,789 58.7 3,737 58.9 55.2 58.4 63.1 1,479 58.3 953 52.4 1,415 50.5 3,859 63.7 [52]

Crassostrea virginica NC_007175 17,244 61.1 3,696 60.1 55.0 60.5 64.7 1,469 63.7 989 57.2 1,567 61.8 832 65.7 [23]

Crassostrea gigas EU672831 18,225 63.4 3,718 62.8 57.1 60.4 71.0 1,314 65.1 2,242 60.1 1,693 63.4 645 69.8 This study

Crassostrea angulata NC_012648 18,225 63.1 3,717 62.4 57.0 60.3 69.8 1,315 65.2 2,244 60.1 1,691 63.3 643 69.5 This study

Crassostrea sikamea NC_012649 18,243 63.4 3,717 62.7 57.1 60.1 70.9 1,314 64.8 2,232 60.2 1,694 63.4 655 71.5 This study

Crassostrea hongkongensis NC_011518 18,622 65.3 3,701 64.5 58.3 60.0 75.3 1,317 64.8 2,264 61.6 1,704 64.6 608 77.6 [25]

Crassostrea ariakensis NC_012650 18,414 62.9 3,699 62.5 57.0 60.3 70.3 1,318 63.7 2,253 59.4 1,698 63.1 716 67.4 This study

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4 -O / 1

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\0 4 4

Figure 2 Mitochondrial genome rearrangements in bivalves and representatives from other molluscan classes. Allgenes are transcribed from left-to-right except those indicated by underlining, which are transcribed from right to left. The bars show identicalgene blocks. The arrows indicate gene translocation and the circling arrows indicate gene inversion. The grey blank boxes represent the uncompleted sequences. The non-coding regions are not presented and gene segments are not drawn to scale.

gene arrangement in Mollusca. At high taxonomic levels, the gene order tends to be conserved across poly-placophoran, cephalopods and gastropods. The gene order of K. tunicata differs from that of the common octopus Octopus vulgaris by merely the inversion of trnP and translocation of trnD. Gene order of other cephalopods resembles that of O. vulgaris with some translocations of tRNA or the switch of large gene blocks [9]. Additionally, the gene arrangement of K. tunicata differs from that of the blacklip abalone Halio-tis rubra merely by the inversion of trnP plus transposition of four tRNAs.

In comparison, however, mt genomes of Bivalvia show tremendous gene rearrangements. To date, all bivalves whose mt genomes are available belong to three subclasses: Palaeoheterodonta, Heterodonta and Pteriomor-phia. Gene order in freshwater mussel Lampsilis ornata (Palaeoheterodonta: Unionoida) is nearly identical to that of it sister species Inversidens japanensis except for the translocation of several tRNAs, and protein-coding genes nad2 and nad3 (Figure 2) [10]. In addition, L. ornata genome contains the atp8 gene which is absent in I. japanensis (Data not shown). The remaining bivalves whose mt genomes have been sequenced are marine species, where the gene order is dramatically rearranged. Comparing the gene arrangement between Meretrix petechialis and Venerupis philippinarum (Het-erodonta: Veneridae), they share four identical gene blocks: two large blocks cox1-L1-nad1-nad2-nad4L-I and cox2-P-cob-rrnL-nad4-H-E-S2-atp6-nad3-nad5, and two small blocks only containing tRNA genes. They share only one gene block cob-rrnL-nad4-atp6 with Acanchocardia tuberculata of the same order. Meanwhile, A. tuberculata and Hiatella arctica share only two small gene blocks trnM-W and nad2-cox1. Species sequenced in Pteriomorphia belong to three orders: Mytiloida, Ostreoida and Pectinoida. Few gene blocks are shared between any two pairs. Among the four scallops of the family Pectinidae (Pectinoida), gene arrangement of Mizuhopecten yessoensis closely resembles that of Chlamys farreri. They share three large gene blocks nad4L-nad6-L2-cob, cox3-K-F-Q-E-atp6-cox2-nad2-T-P-I-L1-M-nad3-nad4 and N-nad1-R-rrnL-M. But their gene order is very different from that of the other two scallops, despite being members of the same family. The two genomes of C. farreri and Argopecten irradians only show three small shared gene blocks nad6-L2-cob, nad3-nad4 and nad1-R-rrnL, while A. irradians and P. magellanicus only share one gene block L2-cob-cox2.

On the other hand, the mt gene order in the six Asian Crassostrea oysters is completely identical to each other (Figure 2). This is highly unusual considering the tremendous rearrangement observed in other groups of Bivalvia. Even within the same genus, comparison

between the Asian Crassostrea oysters of the Pacific and the American oyster C. virginica of the Atlantic Ocean show that gene order has been drastically rearranged. Gene order of the protein-coding genes (PCGs) is the same, while several tRNAs has translocated after the divergence between the Asian and American species. The six Asian Crassostrea oysters have duplicated gene trnK, trnQ and rrnS compared with the American oyster. They differ from the Atlantic sister species by as many as nine tRNA changes (6 transpositions and 3 duplications), and furthermore, they differ significantly from S. mordax in PCGs. If the tRNA is not considered, they share three PCGs blocks cox1-cox3-cob-cox2, nad3-nad1-nad4L, nad5-nad6 and atp6-nad2. It is amazing that Crassostrea sister species from the two oceans have so much difference in gene order and close relatives even have more difference in gene order, while gene order is completely conserved in the six Crassostrea species from Asia. This raises the questions what causes rearrangements of mt genomes and how do they evolve over time. Additional studies of mt genomes of other oyster species would be interesting to see if this pattern should preserve, which may further our understanding of mt genome evolution.

Protein-coding genes

Of the 13 typical PCGs (cox1-cox3, nad1-nad6, nad4L, cob, atp6 and atp8), twelve genes were determined and the atp8 gene was absent in all oyster genomes. All PCGs are encoded on and transcribed from the same strand. These features have been observed in all other marine bivalve genomes published so far except for

H. arctica where the atp8 gene has been reported, and for two species of Palaeoheterodonta (L. ornate and

I. japanensis) where genes are coded on both strands (Figure 2). Thus, coding genes on the same strand and missing atp8 gene are the most distinct features of marine bivalve mt genomes, though from a recent publication from Breton et al [57] this might only hold true for Ostreoida and a few other bivalves. The two Palaeoheterodonta species that use both strands for coding are the only known members of Bivalvia showing significant conservation in gene order with other molluscan classes that also use both strands for coding. We suspect that coding on both strands may be inhibitory to mt genome rearrangement, and marine bivalves show a tremendous amount of mt genome rearrangement because they only use one strand for coding. Rearranging a genome with dual-strand coding may be more complicated and cause more harm than rearranging a genome that codes on one strand.

Mt genomes often use a variety of non-standard initiation codons [44]. In some cases, identification of the very clear initiation codon is difficult when several

alternatives are inferred representing the start of coding sequence in a region. Most of PCGs initiate with the standard start codon ATG. Standard start codon ATA is used for cox3 in C. virginica, nad4 in C. hongkongensis and nad4L in C. sikamea and in C. ariakensis. Nonstandard initiation codon GTG is used for nad5 in C. ariakensis, and ATT is used for nad6 in C. hongkongensis (Table 2). There are no obvious patterns in termination codon usage; the usage frequency of stop codon TAG is similar to that of TAA. Incomplete termination codons T and TA were also used. Termination codon TAA is used in 9 PCGs of C. virginica; if the incomplete termination codon is considered, the number of TAA is up to eleven. The PCGs with identical termination codon in the six genomes are nadl and nad4L, which ended with TAA and incomplete stop codon T, respectively (Table 2).

The number of amino acids coded by each of the mt genomes is approximately equal. Excluding the stop codons, the C. virginica mtDNA encodes the least amino acids (3,696), while the C. gigas mtDNA encodes the most amino acids (3,718). Oysters with the lowest (60.1%) and highest (64.5%) A+T compositions of protein-coding region are C. virginica and C. hongkongensis, respectively. Similarly, oysters showing the lowest and highest A+T content of the first and the third positions are also C. virginica and C. hongkongensis: 55.0% and 58.3% for the first position, and 64.7% and 75.3% for the third position, respectively. However, the A+T content of the second position is approximately the same in the six genomes ranging from 60.0% in C. hongkongensis to 60.5% in C. virginica. It is obvious that the A+T content of the third codon position is higher than that of the

first and the second positions. The genomic features of 19 bivalve sequences are presented in Table 1. The statistics of A+T content, start and stop codon, and amino acid number of PCGs in C. iredalei is already described in Wu et al's paper [54].

Nonsynonymous and synonymous substitutions

The estimation of nonsynonymous (Ka) and synonymous (Ks) substitution rates is of great significance in understanding evolutionary dynamics of protein-coding sequences across closely related species [55,56]. To detect the influence of selection pressure in Crassostrea species, the numbers of Ka and Ks were calculated and their ratios were plotted for all pairwise comparisons among the seven oysters (Figure 3 and additional file 2: Table S2). The ratio of Ka/Ks in all 12 protein-coding genes varied from 0.001 for cox2 in C. angulata_C. sikamea and C. gigas_C. sikamea to 0.2657 for nad6 in C. angulata_C. ariakensis, which supports the existence of different mutation constraints among genes. Most of the amino acid substitutions are localized in the NADH complex genes. It suggests a relaxation of purifying selection in the nad6, nad2, nad3, nad5 genes compared with the more conservative genes such as coxl, cox2 and atp6.

Transfer and ribosomal RNA genes

The mt genomes of the six Asian Crassostrea oysters encode 25 tRNA genes. In addition to the 22 typical tRNAs, there are three duplications of trnM, trnK and trnQ. In comparison, however, there is no duplication of trnK and trnQ in C. virginica. The tRNA structure of C. gigas is referred to as the standard when the nucleotide

Table 2 Comparison of gene length, initiation codon and termination codon in Crassostrea mt genomes

Cgi Can Csi Cho Car Cvi Cgi Can Csi Cho Car Cvi Cgi Can Csi Cho Cai Cvi

coxl 538 538 538 538 538 540 ATG ATG ATG ATG ATG ATG TAG TAG TAA TAA TAA TAA

cox2 233 233 233 233 233 230 ATG ATG ATG ATG ATG ATG TAA TAG TAA TAG TAG TAA

cox3 291 291 291 287 287 290 ATG ATG ATG ATG ATG ATA TAG TAA TAG TAA TAA TA-

nadl 311 311 311 311 311 311 ATG ATG ATG ATG ATG ATG TAA TAA TAA TAA TAA TAA

nad2 332 332 332 332 332 331 ATG ATG ATG ATG ATG ATG TAG TAG TAA TAG TAG TAA

nad3 116 116 116 116 116 117 ATG ATG ATG ATG ATG ATG TAG TAG TAG TAG TAA TAA

nad4 450 449 449 449 449 449 ATG ATG ATG ATA ATG ATG TAA TAA TAG TAG TAG TAA

nad4L 94 94 94 93 94 93 ATG ATG ATA ATG ATA ATG T- T- T- T- T- T-

nad5 556 556 557 556 556 555 ATG ATG ATG ATG GTG ATG TAG TAG TAG TAA TAG TAA

nad6 158 158 158 158 159 153 ATG ATG ATG ATT ATG ATG TAG TAG TAG TA- TAA TAA

cob 412 412 411 401 400 403 CTA CTA CTG ATA TTA TTA TAG TAG TAA TAA TAA TAG

atp6 227 227 227 227 224 224 ATG ATG ATG ATG ATG ATG TAA TAA TAG TAG TAG TAA

rrnL,* 601 602 602 605 606 748

rrnL2* 713 713 712 712 712 721

rrnS,* 1037 1038 1037 1074 1070 989

rrnS2* 1205 1206 1195 1190 1183 0

*rrnL1 and rrnL2 indicate the two parts of 5' half and 3'half rrnL, respectively; rrnS1 and rrnS2 indicate the duplication of rrnS.

cox J —>—cox2 cox3 —X—cob —X—atp6 —•—until —I—nad2 —-—nad3 ———nadJ nad4L nad5 nad6

Figure 3 The ratio of nonsynonymous and synonymous substitutions (Ka/Ks) estimated in all 12 protein genes of seven Crassostrea oysters.

variation in other four Asian oysters is examined (C. ireda-lei is not examined as it was not sequenced in this study). Nucleotide variations including transition/transversion and insertion/deletion mostly occur at DHU and TYC loops (Additional file 3: Figure S1).

Duplication of tRNA is common in molluscs. A second trnM is also presented in the mussel Mytilus edulis, M. galloprovincialis and M. trossulus [45-47], and the clam V. philippinarum, H. arctica and A. tuberculata [48]. In P. magellanicus, there are up to ten copies of trnM [42]. In M. trossulus mtDNA, an additional copy of trnQ has also been reported [47]. The anticodon usage of oyster genomes is congruent to the corresponding tRNA of other molluscs with one exception. The anticodon of two trnMs in the oysters is CAU while that of two trnMs is CAU and UAU in Mytilus. The difference in anticodon corresponds to the third wobble position.

The boundaries of both the small and the large ribo-somal genes were determined by BLAST with the revised annotation of C. gigas genome [23]. The rrnL gene is split into two segments, one segment of 5' end is distributed between trnQ1 and trnN, and the other segment of 3' end is located between cox1 and cox3. The split rrnL is first reported in C. virginica, but so far not reported in other metazoan mt genomes. The mt

genome of S. mordax also has the split rrnL , however, it does not have the duplicated rrnS. The duplication of rrnS is only found in the six Asian Crassostrea oysters and the similarity between intraspecies and interspecies is high (95-100%) in the conserved 940 nucleotides at the center of rrnS sequences. The size of rrnL in the Asian Crassostrea oysters is nearly equal, and smaller than that in C. virginica (Table 1). The length of rrnS in C. virginica is 989 bp, while total length of rrnS in the Asian Crassostrea oysters varies from 2,232 bp (C. sika-mea) to 2,264 bp (C. hongkongensis).

Non-coding regions

As in most bivalves, the oyster mtDNAs contain a large number of unassigned nucleotides. There are more than 30 non-coding regions throughout the seven Crassostrea genomes. The unassigned nucleotides vary from 1,788 bp in C. gigas to 5,873 bp in C. iredalei. The proportion of the unassigned nucleotides in the whole genome varies from 9.81% in C. gigas to 26.17% in C. iredalei. The largest non-coding region in the Asian oysters is located between gene trnG and trnV, while it is between gene trnP and trnG in C. virginica. The sequence of each corresponding non-coding region from the six Asian Cras-sostrea oysters was listed separately and regions large than 30 nucleotides were aligned using ClustalX 1.83.

There are 14 non-coding regions aligned (See the Supplement to the non-coding regions). The alignment shows that there are sequence conservation in the non-coding region, especially, higher sequence conservation in the regions between trns2 and trnP, trnV and nad2. The sequence similarity among species in the non-coding region is positively correlated with their relatedness.

Phylogenetic analysis and divergence time estimation

Phylogenetic analysis using all mt coding sequences provides clear evolutionary relationships among the seven Crassostrea and one Saccostrea oyster species (Figure 4). C. gigas is first clustered with C. angulata and then united with C. sikamea, meanwhile C. hongkongensis and C. ariakensis formed a clade. Finally, C. iredalei with other five Crassostrea oysters form a large Asian clade. Together with Asian Crassostrea oysters, the C. virginica formed the Crassostrea clade. S. mordax diverged early from Crassostrea oysters and was positioned at the base of the large oyster clade. All the phylogenetic relationships are supported with high values. The close relationships between C. gigas and C. angulata, and C. hongkongensis and C. ariakensis are clearly demonstrated on the phylogenetic trees (Figure 4), which has been the subject of debate for some time [14,12,22]. The complete mt genomes of C. gigas and C. angulata differed by 3%, providing strong support for their status as two independent species.

The first appearance of Gastropoda and Bivalvia in fossil record is approximately 542 Myr ago [40],

providing the calibration constraint for divergence estimation. Divergence time estimates between species and/ or clades are given in Table 3 and Figure 5. Our estimation shows that C. virginica and the Asian oysters diverged about 82.7 Myr ago. The six Asian Crassostrea oysters started their divergence about 42.8 Myr ago with the separation of C. iredalei from other species first. Other two large clades C. hongkongensis and C. ariaken-sis, and C. sikamea, C. gigas and C. angulata diverged 28.8 Myr ago. C. hongkongensis and C. ariakensis diverged 22.3 Myr ago. In the other clade, C. sikamea diverged from C. gigas and C. angulata 16.5 Myr ago. The two most closely related species, C. angulata and C. gigas, diverged about 2.7 Myr ago.

These estimates indicate that, relative to the divergence with C. virginica about 83 Myr ago, the speciation of the six Asian Crassostrea species is rather recent or 3-43 Myr ago. Still, it is difficult to reconcile molecular divergence with genome rearrangement data observed here. Assuming the divergence time estimates are correct, it is intriguing that the mt genomes of C. virginica and the Asian species diverged with nine structure rearrangements (6 transpositions and 3 duplications) in about 83 Myr, while there was not a single rearrangement event among the six Asian Crassostrea species in the past 43 Myr. During the same time frame, numerous rearrangement events occurred among scallop species (Figure 2). Thus, the complete conservation of gene order among the Asian Crassostrea oysters in the past 43 Myr is highly unusual, given the amazing rate of mt genome rearrangements in marine bivalves in general

Figure 4 Phylogenetic trees based on the concatenated amino acid (a) and nucleotide sequences (b) of 12 protein-coding genes (The branch length determined with NJ analysis). The chiton Katharina tunicata was used as outgroup. NJ (left number) and ML (right number) bootstrap values are given for each branch.

Table 3 Optima (minima and maxima) in millions of years derived for each clade

Clade name Mean SD Minima Maxima

(Myr) (Myr) (Myr) (Myr)

Aplysia californica : Pupa strigosa 257.434 18.841 220.719 295.515

Albinaria caerulea: (Aplysia californica : Pupa strigosa) 355.753 18.063 320.518 391.260

Cepaea nemoralis : (Albinaria caerulea : (Aplysia californica : Pupa strigosa)) 390.342 18.392 354.283 426.306

Mytilus edulis : Mytilus galloprovincialis 2.474 0.553 1.556 3.717

Chlamys farreri : Mizuhopecten yessoensis 46.142 5.483 36.418 57.765

Placopecten magellanicus : (Chlamys farreri : Mizuhopecten yessoensis) 88.175 9.537 70.950 108.724

Argopecten irradians : (Placopecten magellanicus : (Chlamys farreri : Mizuhopecten yessoensis)) 113.296 11.374 92.757 137.153

Crassostrea angulata : C. gigas 2.723 0.397 2.025 3.577

C. sikamea : (C. angulata : C. gigas) 16.469 2.040 12.770 20.885

C. hongkongensis : C. ariakensis 22.316 2.786 17.333 28.334

(C. hongkongensis : C. ariakensis): (C. sikamea : (C. angulata : C. gigas)) 28.781 3.448 22.615 36.222

C. iredalei : ((C. hongkongensis : Cr. ariakensis): (C. sikamea : (C. angulata : C. gigas))) 42.770 5.051 33.622 53.649

C. virginica : (C. iredalei : ((C. hongkongensis : C. ariakensis): (C. sikamea : (C. angulata : C. gigas)))) 82.651 9.015 66.209 101.967

Saccostrea mordax (C. virginica : (C. iredalei : ((C. hongkongensis : C. ariakensis): (C. sikamea : 109.049 11.125 88.800 132.464

(C. angulata : C. gigas)))))

Ostreoida: Pectinoida 421.475 18.890 384.824 4.58.57

Mytiloida: (Ostreoida: Pectinoida) 479.361 16.121 448.075 510.635

Gastropoda: Bivalvia 540.866 11.359 522.899 560.657

and within the genus Crassostrea itself. While this finding provides strong evidence for recent speciation of the six Asian Crassostrea species, it also indicates that mt genome rearrangement may not be strictly a function of time, but constrained by other factors. Detailed mechanisms of mt genome rearrangement are unknown. We suspect that coding on both strands may be one of the factors inhibiting mt genome rearrangement. This hypothesis is supported by the fact that the only two bivalve molluscs (L. ornata and I. japanensis) with dualstrand coding have relatively fewer rearrangements of gene order compared with other molluscan classes (Figure 2). All marine bivalves use one strand for coding and show tremendous rearrangements. The extensive rearrangement of bivalve mt genomes may be a consequence of coding on one strand. Furthermore, the fact that massive rearrangements in marine bivalves have not disrupted single-strand coding or changed the direction of transcription suggests that the rearrangements are not caused by inversion or reverse transposition. Thus, transposition or "tandem repeat-random loss"[49] may be important for mt genome rearrangement [29].

Dual-strand coding may not be the only factor limiting mt genome rearrangement, as it cannot explain the absence of rearrangements in the six Asian oysters over 43 Myr. We speculate that some other unknown features in mt genomes of the six Asia species may also limit genome rearrangements. The most noticeable structure change is the duplication of rrnS in the six Asian species, though it is difficult to determine if it has

any effects on genome rearrangement at current stage. Further studies on the mt genomes of other oysters and marine bivalves may shed light on the origin and evolution of genome rearrangements. Gene order data have been shown to be valuable in phylogenetic analysis [3-6]. While our analysis demonstrates the power of rearrangement data, it also argues for a better understanding of mt genome rearrangement before using them to infer divergence time.


Asia Pacific has long been suspected to be the center of oyster speciation. Our analysis of complete mt genomes provides strong evidence that Crassostrea oysters have diversified in Asia and the divergence was rather recent or within the last 3-43 Myr. The complete conservation of gene order in the six Asian Crassostrea species over a period of 43 Myr is highly unusual given the remarkable rate of rearrangements in their sister species and other bivalves during the same time frame. It provides strong evidence, in addition to sequence data, for the recent speciation of Crassostrea oysters in Asia. It also indicates that changes in mt gene order may not be strictly a function of time but subject to other constraints that are presently not well understood.


Sample collection and DNA extraction

Specimens for the five Asian Crassostrea species were mostly collected from coastal waters of China, except

Figure 5 A phylogenetic tree with divergence time (in million years) of 19 molluscan species rooted with the chiton Katharina tunicata as determined by Bayesian phylogenetic analysis.

that C. gigas was collected from a cultured population in Oregon, USA. C. gigas is a native species of Asia and was introduced to the West Coast of USA for aquaculture production. The oyster ID, geographic origin and collection date of each specimen are provided in Table 4. All oysters were identified according to their morphological characteristics first and confirmed with species-specific molecular markers [21]. Total genomic DNA was extracted from ethanol-fixed tissue with the CTAB method and dissolved in TE (10 mM Tris-HCl 1 mM EDTA pH 8.0) buffer before being stored at -20°C.

PCR amplification and DNA sequencing

Four pairs of primers were designed to amplify the complete mt genomes of C. gigas and C. angulata according to the sequence of C. gigas in GenBank (Additional file 3: Table S3). Sequencing primers were designed at intervals of about 500 bp. All PCR products were directly sequenced by primer-walking. Partial sequences of C. sikamea and C. ariakensis were obtained with the combined primers of the amplifying and sequencing primers

designed for C. gigas. The remaining gaps were amplified with the species-specific primers designed according to the obtained sequences. As for C. hongkongensis, two short fragments of cox1 and cox2 were first amplified with the universal primer sets of LCO1490+ HCO2198 [53] and cox2F+cox2R [26,27]. In addition, partial sequence of nad5 was amplified with the primers of C.

Table 4 Sample information for the five Crassostrea species studied

Species Oyster ID Origin Date

C. gigas ORCg-4 Cultured, Oregon, USA 06/2005

C. angulata Cangtaiwh-9 Wild, Taiwan, China 06/2006

C. sikamea NT-521 Wild, Nantong, Jiangsu, China 04/2006

C. hongkongensis* Hainan big#1 Cultured, Lingao, Hainan, China 08/2005

C. ariakensis YKy101 Wild, Yingkou, Liaoning, China 08/2006

* This data has been published in Ren et al., 2009 [25].

gigas. Then, the whole mt genome was amplified based on three pairs of primers (Additional file 4: Table S3).

PCR reactions were performed with a Mastercycler gradient machine (Eppendorf). The cycling was set up with an initial denature step at 94°C for 2 min, followed by 35 cycles comprising denaturing at 94°C for 20 sec, annealing at 52-58°C for 1 min and elongation at 68°C or 72°C for 6 or 10 min depending on the expected length of the PCR products. The process was completed with a final elongation at 72°C for 10 min. The reaction volume amounted 25 ^l containing 18.8 ^l sterile deio-nized water, 2.5 ^l 10xLA PCR buffer (Mg2+ plus, Takara), 1 ^l dNTP mix (10 mM each), 1 ^l each primer (5 ^M), 0.2 ^l LA Taq DNA polymerase (5 U/^l, Takara) and 0.5 ^l DNA template (50 ng/^l). A negative control (no template) was included during each PCR run. PCR products were directly purified with Multi-Screen-PCR96 Filter Plate (Millipore) and sequenced with ABI 3730x1 DNA Analyzer (Applied Biosystems).

Sequence analysis and gene annotation

Raw sequencing reads were first processed using Phred with the quality score 20 and assembled in Phrap with

default parameters [28,29]. Then, all assemblies and sequence quality were verified manually in Consed to remove misassemblies [30]. The accurate boundary of each gene was determined according to the annotated C. gigas mt genome [23] with minor revisions. The tRNA genes were identified by tRNAscan-SE 1.21 [31] employing the cove only search mode and the invertebrate mt genetic code. The ratio of nonsynonymous and synonymous substitutions rates (Ka/Ks) was estimated with Ka_Ks calculator in all 12 protein genes of seven Crassostrea oysters.

Phylogeny analysis and divergence time estimation

Nineteen molluscan mt genomes including four obtained in this study were used for phylogenetic analysis (Table 5). The black chiton Katharina tunicata (Polyplacophora) was rooted as the outgroup. The amino acid sequence from each of 12 protein-coding genes (excluding atp8) was aligned separately using ClustalX 1.83 [33], and then trimmed to the same length and concatenated for further analysis. The nucleotide sequence was substituted from the concatenated amino acid alignment. The final nucleotide sequence consisted

Table 5 List of taxa used in the phylogenetic analysis


GenBank Accession Number


Katharina tunicata Gastropoda

Aplysia californica

Pupa strigosa

Albinaria caerulea Cepaea nemoralis Bivalvia



Mytiius edulis

Argopecten irradians

Placopecten magellanicus

Mizuhopecten yessoensis

Chlamys farreri

Crassostrea virginica

Crassostrea gigas

Crassostrea angulata

Crassostrea sikamea

Crassostrea hongkongensis

Crassostrea ariakensis

Crassostrea iredalei

Saccostrea mordax

Neoloricata; Chitonida; Acanthochitonina; Mopaliidae NC_001636

Orthogastropoda; Apogastropoda; Heterobranchia; Euthyneura; Opisthobranchia; Anaspidea; NC_005827 Aplysioidea; Aplysiidae;

Orthogastropoda;Apogastropoda; Heterobranchia; Euthyneura; Opisthobranchia; Architectibranchia; NC_002176 Acteonoidea; Acteonidae

Pulmonata; Stylommatophora; Sigmurethra; Clausilioidea; Clausiliidae NC_001761

Pulmonata; Stylommatophora; Sigmurethra; Helicoidea; Helicidae NC_001816

Bivalvia; Pteriomorphia; Mytiloida;Mytiloidea; Mytilidae NC 006886

Bivalvia; Pteriomorphia; Mytiloida; Mytiloidea; Mytilidae NC _006161

Bivalvia; Pteriomorphia; Pectinoida;Pectinoidea; Pectinidae

Bivalvia; Pteriomorphia; Pectinoida;Pectinoidea; Pectinidae NC _007234

Bivalvia; Pteriomorphia; Pectinoida;Pectinoidea; Pectinidae NC _009081

Bivalvia; Pteriomorphia; Pectinoida;Pectinoidea; Pectinidae

Bivalvia; Pteriomorphia; Ostreoida;Ostreoidea; Ostreidae NC _007175

Bivalvia; Pteriomorphia; Ostreoida;Ostreoidea; Ostreidae EU672831

Bivalvia; Pteriomorphia; Ostreoida;Ostreoidea; Ostreidae NC _012648

Bivalvia; Pteriomorphia; Ostreoida;Ostreoidea; Ostreidae NC _012649

Bivalvia; Pteriomorphia; Ostreoida;Ostreoidea; Ostreidae NC .011518

Bivalvia; Pteriomorphia; Ostreoida;Ostreoidea; Ostreidae NC _012650

Bivalvia; Pteriomorphia; Ostreoida;Ostreoidea; Ostreidae NC _013997

Bivalvia; Pteriomorphia; Ostreoida;Ostreoidea; Ostreidae NC _013998

of 9,537 sites. Two phylogenetic reconstruction approaches were performed including Maximum Likelihood (ML) with PHYML 3.0 [34] and Neighbor-Joining (NJ) with MEGA 4.0 [35]. The assessments of node reliability in both the ML and NJ analyses were done by using 1,000 bootstrap replicates.

Molecular estimates of divergence time for multiple gene data were performed using the relaxed Bayesian molecular clock approach as implemented in MULTIDIS-TRIBUTE package [36]. Key features of this program are that posterior distribution of molecular time estimates and rates of molecular evolution are approximated while simultaneously taking account of uncertainty in branch length estimates from each gene [37]. The F84+gamma evolution model, incorporating different rates of transition/transversion, variable nucleotide frequencies, and nucleotide variation across sites, was used to estimate the maximum likelihood parameter in PAML version 3.15 [38]. The Multidivtime program used the output from Estbranches analyses to estimate node divergence times for the ingroup, given time constraints, various parameters, and estimated priors. The median of all the tip-to-root branch lengths was calculated using ape and LAGOPUS package in R project [39]. Gamma priors were chosen as the following procedure outlined in the Multidivtime manual: expected time between the tip and the ingroup root (rttime) = 542 Myr (million years) ago, with standard deviation (SD) = 30 Myr ago; rate of the root node (rtrate) and its SD = 0.46 substitution per site per 100 Myr determined as the median of all the tip-to-root branch lengths divided by rttime; and rate of change between ancestral and descendant nodes (brownmean) = 0.18. Katharina tunicata was considered to be outgroup to Bivalvia and Gastropoda as required by the program. All divergence time were calculated assuming the topology of the consensus tree, which was derived from previous ML/Bayesian analyses based on protein sequences. The first appearance of skeletons in the fossil record, indicating the maximum for the origin of Gastropoda + Bivalvia is approximately 542 Myr ago, provides the calibration constraint for divergence estimation [40]. The parameters for the Markov Chain Monte Carlo (MCMC) simulation were set as follows: number of samples = 10,000, sample frequency = 200 and burn-in period = 2,000. Finally, Multidivtime analyses considering var-iance-covariance matrices from each gene partition were run twice ensure convergence, each one starting with a different random initial seed number.

Additional material

Additional file 1: Table S1: Organization of the mitochondrial genome of six oysters.

Additional file 2: Table S2: The ratio of nonsynonymous and synonymous substitutions (Ka/Ks) estimated with Ka_Ks calculator in all 12 protein genes of seven Crassostrea oysters.

Additional file 3: Figure S1: Comparison of the potential secondary structures of the 25 inferred tRNAs among five Crassostrea oyster mtDNAs.

Additional file 4: Table S3: Major primers used in amplifying the mitochondrial genomes.


We thank Mengjie Qiu for DNA sequencing. We thank the editors and the anonymous reviewers for their insightfulcomments. This work was supported by the Bairen program of Chinese Academy of Sciences (to BL) and by the MFG Fund of Chinese Academy of Sciences.

Author details

''Key Laboratory of ExperimentalMarine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. 2Center of Systematic Genomics, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. 3Haskin Shellfish Research Laboratory, Institute of Marine and CoastalSciences, Rutgers University, NJ 08349, USA. 4Current Address: Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing 100101, China. ^Current Address: Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

Authors' contributions

JR did PCR, sequencing and initialanalysis; FJ did some data analysis; XG and XL provided the samples; BL, XG and XL conceived the study; JR, BL and XG wrote and revised the manuscript. Allauthors read and approved the final manuscript.

Received: 7 May 2010 Accepted: 28 December 2010 Published: 28 December 2010


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Cite this article as: Ren et al.: Unusual conservation of mitochondrial gene order in Crassostrea oysters: evidence for recent speciation in Asia. BMC Evolutionary Biology 2010 1 0:394.