CC BY
0Hindawi Publishing Corporation
Journal of Nanomaterials
Volume 2014, Article ID 431925, 10 pages
http://dx.doi.org/10.1155/2014/431925
Research Article
Synthesis and Characterization of Different Crystalline Calcium Silicate Hydrate: Application for the Removal of Aflatoxin B1 from Aqueous Solution
Lu Zeng, Ligang Yang, Shuping Wang, and Kai Yang
College of Materials Science and Engineering, Chongqing University, Chongqing 400044, China Correspondence should be addressed to Lu Zeng; zool@foxmail.com Received 31 December 2013; Accepted 17 February 2014; Published 17 March 2014 Academic Editor: Ming-Guo Ma
Copyright © 2014 Lu Zeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Different crystalline calcium silicate hydrates (CSH) were synthesized under specific hydrothermal conditions and several methods were used to analyze samples. Amorphous calcium silicate hydrates (ACSH) mainly consists of disordered calcium silicate hydrate gel (C-S-H gel) and crystalline calcium silicate hydrates (CCSH) consists of crystallized tobermorite. The adsorption of carcinogenic aflatoxin B1 (AFB1) onto ACSH and CCSH was investigated. The adsorption kinetics was studied using pseudofirst-order and pseudo-second-order kinetic models and intraparticle diffusion model. The pseudo-second-order model provided the best correlation and the intraparticle diffusion controlled the adsorption process of AFB1 onto CCSH. Adsorption isotherm parameters were obtained from Langmuir and Freundlich and the adsorption data fitted to Freundlich much better. Based on the results of N2 adsorption/desorption, adsorption kinetics, and adsorption isotherms, the adsorption mechanism of AFB1 onto CCSH was developed. All results indicate that CCSH has a great potential to be a safe, easy-made, and cost-effective material for the control ofAFB1 contamination.
1. Introduction
The synthesis of calcium silicate hydrate (CSH) under the hydrothermal condition has been extensively studied to manufacture some functional materials. Under different hydrothermal conditions, varieties of CSH with different structures can be prepared, such as amorphous calcium silicate hydrate (ACSH) and crystalline calcium silicate hydrate (CCSH). ACSH, for example, calcium silicate hydration gel (C-S-H gel), consists of monomer silicate anions with silanol groups [1]. CCSH, for example, tobermorite and xonotlite, is built up of layers of Ca polyhedra and condensed by wollastonite-type silicate chains on both sides [2, 3]. CSH is used as functional Materials generally, especially as an adsorbent due to its high specific surface area and a large number of structural sites. In the study of Maeda and Ishida [4], slurries consisting of diatomaceous earth and slaked lime were hydrothermally reacted to synthesize composite C-S-H gel, which has superior dye-adsorption ability. A synthetic adsorbent of CCSH has been found highly effective for
endotoxin removal from aqueous solutions [5]. Katsumata et al. [6] removed natural organic polyelectrolyte (humic and fulvic acids) and their metal complexes by adsorption onto xonotlite. Siauciunas et al. [7] evaluated the effects of CO2 chemisorptions on synthetic tobermorite. Novel technique for phosphorus recovery from aqueous solutions using ACSH was reported recently [8], and also CCSH with a low Ca/Si molar ratio of 0.8-1.0, such as tobermorite [9] and xonotlite [10], has beenused for phosphorus recovery. Both of them are beneficial materials for phosphorus recovery and recycling. Moreover, CSH plays an essential role in fixing heavy metals by ways of ion-exchange, adsorption, and chemical restraint. Heavy metals of Nd(III), Zn(II), Cd(II), Cr(IV), and Sr(II) uptake by CSH have been reported in the literature [11-15].
Mycotoxins are secondary metabolites of filamentous fungi and naturally occur in food. They represent a large group of different substances produced by different myco-toxigenic species. Aflatoxins are the most common myco-toxins and aflatoxin B1 (AFB1) has been demonstrated to be carcinogenic to animals and humans [16]. At present, the
Table 1: Hydrothermal synthesis conditions.
Samples Ca/Si w/s Incubating time (h) Temperature (°C)
ACSH 1.0 5 8 120
CCSH 1.0 10 12 185
most probable approach for controlling AFB1 contamination is the addition of nonnutritive adsorbent materials to the diet. Minerals such as montmorillonite, sepiolite, zeolite, and kaolinite are the main materials which have been used as the adsorbents of AFB1 [17]. However, these materials are nonrenewable and may be insecure as the natural substance could suffer some contamination (e.g., heavy metals); the US Food and Drug Administration (FDA) stated that "The use of sodium aluminosilicate and hydrated calcium sodium aluminosilicate as binders for mycotoxins is not considered to be generally recognized as safe" [18], so their application is limited in practice. To control AFB1 contamination, it is essential to develop a simple, safe, and cost-effective adsorbent.
Till now, CSH has not been used to bind AFB1 and the structures of CSH which can impact the adsorption capacity have not been studied too. In this study, different crystalline CSH were prepared under specific hydrothermal conditions and samples were characterized by several methods. The adsorption capacities of CSH for the removal of AFB1 from aqueous solutions were investigated and the adsorption mechanism of AFB1 on CCSH was explained.
2. Materials and Methods
2.1. Synthesis and Characterization of CSH. CSH was synthesized in an autoclave (Weihai Chemical Machinery Co. Ltd.). For preparation of CSH, the calcium oxide (99.99% CaO, Aladdin Co. Ltd.) and silicon oxide (99.99% SiO2, Aladdin Co. Ltd) were mixed according to a certain proportion by molar (Ca/Si). The mixture was added to the water with a certain ratio of water-to-solid by mass (w/s), and then incubated at the set temperatures for several hours to manufacture ACSH and CCSH; the hydrothermal synthesis conditions are shown in Table 1 [19]. Samples of each synthesis were dried to constant mass in an oven at 80°C and then milled to less than 45 ^m for the adsorption experiments.
ACSH and CCSH were characterized by different methods, including X-ray powder diffraction (XRD), scanning electron microscope (SEM), and N2 adsorption/desorption. XRD was performed on a Rigaku D/max-1200 diffractometer with CuKa radiation, in the range of 5°-70° 20 and at a step size of 0.02°. SEM micrographs were performed using a TESCAN VEGA II scanning electron microscope, operating at the accelerating voltage of 20 kV and taking images at different magnifications. Brunauer-Emmet-Teller (BET) specific surface area (SBET), total pore volumes (Vjotal),and average pore diameter (Dp) of the samples before and after adsorption of AFB1 were measured from N2 adsorption/desorption isotherms at 77 K, using an automatic specific surface area measuring equipment (ASAP, 2020, Micromeritics, USA) after a degassing under vacuum for 8 h at 110°C.
Figure 1: Structure of AFB1.
2.2. Adsorption Experiments. The method of enzyme linked immunosorbent assay (ELISA) was used to measure the amount of AFB1. AFB1 was purchased from Sigma-Aldrich Co and ELISA kits were purchased from R-Biopharm Co. The chemical structure of AFB1 is presented in Figure 1 [20]. Firstly, an AFB1 stock solution (2 ^gmL-1) was prepared, and then 20 mg CSH was added to a tube that was filled with 10 mL AFB1 solution, adjusting the solution pH to 8 by adding 0.1 M phosphate buffer as required. After 2h at 28°C in a temperature controlled shaking water bath pot at a shaking rate of120 rpm, the tube was centrifuged for 10 min at 5000 rpm and then the amount of AFB1 remaining in the supernatant layer of dispersion by the method of ELISA was analyzed. The adsorbance of AFB1 was calculated from the difference between initial and equilibrium concentrations of AFB1. In addition, some experiments were performed at solution temperatures of 301, 306, and 311K to determine the kinetic parameters with different time intervals. In order to determine adsorption isotherms parameters, experiments were carried out at solution temperatures of 301 K with different initial concentration which varied from 0.5 to 3 ^gmL-1.
3. Results and Discussion
3.1. Characterization
3.1.1. XRD Characterization. The XRD patterns of ACSH and CCSH reveal the microstructure variety and comparative XRD patterns of them are presented in Figure 2. As can be seen, the ACSH consists of disordered calcium silicate hydrate (C-S-H) gels with peaks at 3.05, 2.81, and 1.81 A (corresponding to 29.1, 32.2, and 49.9° 20, resp.) and unreactive SiO2 from quartz as well due to the short incubating period. The peak of C-S-H gel at 3.05 A is strong and broad, but the peaks at 2.81 and 1.81 A are weak, indicating a low-crystallinity structure [21]. On the contrary, the main composites of CCSH are crystallized tobermorite. The peaks at 11.30, 3.07, 2.97, 2.80, 1.84, and 1.67 A correspond to the main tobermorite component, especially at 11.30 A (corresponding to 8° 20) which is strong and sharp, reflecting an ordered and high-crystallinity structure [22]. Moreover, the peaks of quartz disappear, indicating more SiO2 was dissolved in the liquid, which can be attributed to the higher reaction temperature and longer reaction time [19].
10 20 30 40 50 60
28 (deg)
* Tobermorite ■ Quartz
t C-S-H
Figure 2: XRD patterns of ACSH and CCSH.
3.1.2. SEM Characterization. The SEM micrographs of ACSH and CCSH are shown in Figure 3. The microappearance is different from each other. The ACSH is agglomerated with some irregular flake and fibril. As a certain amount of water is wrapped in the floc, the particle of ACSH is made up of solids and solutions and its appearance is consistent with the typical characteristics of C-S-H gels [23]. Meanwhile, it can be seen that the CCSH consists of the crystals of a plate form which correspond to the microappearance of tobermorite. It is known that Ca2+ and H2O insertions exist among tobermorite plates and space among tobermorite plates and insertions are referred to as tubular pores [24]. In addition, a small amount of floccules are observed in the micrograph of CCSH due to the residual C-S-H gels, which did not turn into tobermorite.
3.1.3. N2 Adsorption/Desorption. The SBET, Vtotal, and Dp of the samples before and after adsorption of AFB1 were measured. Meanwhile, the adsorbance of AFB1 on ACSH and CCSH (AAFB1) was measured. All of the data were summarized in Table 2. The N2 adsorption/desorption isotherms for samples of original (ACSH and CCSH) and after adsorption of AFB1 (ACSH-AFB1 and CCSH-AFB1) are shown in Figure 4. As can be seen from Figure 4(a), isotherms exhibit Type II behaviors according to the IUPAC classification [25], characteristics of major mesoporous, and a few macroporous adsorbents. Seen from the formed hysteresis loop, the N2 adsorbance in the area of high relative pressure is higher, which fits to the third type of hysteresis loop (H3), indicating slit pores heaped up with flake particles. Meanwhile, compared with ACSH, the N2 adsorbance of CCSH in the area of high relative pressure is much lower, which may be attributed to pores that formed from layer structure [25]. From Table 2, the SBET of ACSH is larger than that of CCSH, because disordered ACSH has a structure of three-dimensional networks, while CCSH mainly has two-dimensional layer structure [26]. Moreover, it is shown in
Table 2: The structure characteristics of samples and adsorbance of AFB1.
Sample SBET (mV ') V^ (cmV) Dp (nm) A AFB1 Wg)
ACSH 89.636 0.469 20.372 274.355
CCSH 57.343 0.247 18.924 949.261
ACSH-AFB1 80.525 0.412 19.861 —
CCSH-AFB1 37.479 0.146 18.121 —
Figure 4(a) that, in the area of low relative pressure, there is a slight difference between the N2 absorbance of ACSH and that of CCSH. As the relative pressure increased, within the mesohigh relative pressure range and particularly within the high relative pressure range, the N2 adsorbance of ACSH is significantly larger than CCSH, indicating a different pore structure, which is the main reason why the Vtotal and Dp of ACSH are larger than that of CCSH.
Figure 4(b) presents the N2 adsorption/desorption isotherms of ACSH-AFB1 and CCSH-AFB1. Compared with the original sample, the SBET, V^, and Dp of ACSH-AFB1 slightly decrease and the N2 adsorbance in the area of low relative pressure reduce, approaching closer to the x-axis, which can be attributed to micropore filling by the adsorbed AFB1. However, in the area of mesohigh relative pressure, the N2 adsorbance does not show any obvious changes, indicating that the mesoporous does not fill with AFB1. As a mesoporous material, it was concluded that the main way of the adsorption of AFB1 onto ACSH is surface adsorption. On the contrary, the SBET, Vtotal, and Dp of CCSH-AFB1 all are reduced significantly compared with the original sample and the curve approaches more closer to the x-axis in various relative pressure ranges. Furthermore, the adsorbance of AFB1 onto CCSH is much higher than that of ACSH. Such a difference is associated with the existing forms of AFB1 molecules in the layer structure of CCSH. There are two reactive mechanisms between CCSH and organic components [27]: one is the surface adsorption and the other is the interlayer adsorption as organic molecules insert into layers. The adsorbance of interlayer adsorption to the organics is much higher than the surface adsorption. As can be seen from Figure 1, AFB1 belongs to micromolecular organics, after being adsorbed by CCSH; the Sbet
of CCSH-
AFB1 is much smaller than that of the original sample. According to the effect of "pore blockage" when organic molecules fill pores result in the decrease of SBET [28], it indicates that the interlayer adsorption is the dominant adsorption for the changes of CCSH isotherms before and after adsorption of AFB1. Besides, the hysteresis loop of CCSH-AFB1 fits to the second type of hysteresis loop (H2), indicating that "pore blockage" effect changes the pore structure, which forms ink-bottle pore (pores with narrow necks and wide bodies) [25]. Therefore, the interlayer adsorption is the main way for CCSH adsorbing AFB1.
3.2. Adsorption Kinetics. In order to quantify the changes in adsorption with time (Figure 5) and to evaluate kinetic parameters, three models were assessed, including the pseudo-first-order kinetic model, the pseudo-second-order
Figure 3: SEM micrographs of ACSH (a) and CCSH (b).
Relative pressure (p/p0)
-e- ACSH — CCSH
Figure 4: (a) The N2 adsorption-desorption isotherms for ACSH and CCSH-AFB1.
Relative pressure (p/p°)
-e- ACSH-AFB1 — CCSH-AFB1
CCSH. (b) The N2 adsorption-desorption isotherms for ACSH-AFB1
kinetic model, and the intraparticle diffusion model. The pseudo-first-order kinetic model [29] can be expressed as follows:
lg -<it) = lg qe -
where qe and qt are the amounts of AFB1 adsorbed on CSH (^gg-1) at equilibrium and at different time t (min) and k1 (min- ) is a rate constant for the adsorption process. Kinetic parameters (qe and fc1)were calculated from the intercept and the slope of the pseudo-first-order straight line.
The pseudo-second-order rate expression [30] can be used to describe adsorption processes in which the reaction
rate is proportional to the square of the number of available adsorption sites:
+ —t,
It k2(qe )2 q
where k2 (g mg-1 min-1) is the rate constant and the values of qe and k2 were derived from the intercept and slope of a linear plot of t/qt versus t.
An intraparticle diffusion model of Weber and Morris [31] is shown as
qt = k311'2 + b, (3)
where b represents the intercept and the values of intercept give an idea of the boundary layer thickness which is associated with the boundary layer effect. k3 is the intraparticle
=L 750
0 20 40 60
306 K 311 K
290 -, 280 -270 -260 Л 250 -240 230 220 210
100 120 140 160 180 200 220 t (min)
0 20 40 60 80 100 120 140 160 180 200 220 t (min)
-•- 311K
301 K 306 K
Figure 5: Adsorption kinetics for AFB1 onto CCSH (a) and ACSH (b) at 301, 306, and 311K.
1000 300
900 / 280
850 Iff A
800 -Jg 750- M ^ 260
^ 700 - 240
600 M 220
500 200
0123456789 10 t1/2 (min)
A 301K • 311K
□ 306 K
* 301 K □ 306 K
t1/2 (min)
• 311 K
Figure 6: Intraparticle diffusion plot for AFB1 onto CCSH (a) and ACSH (b) at 301, 306, and 311K.
diffusion constant (mgg 1 min 1/2 ) and the values of k3 and b were obtained from the slope and intercept of a linear plot of qt versus i1/2 (Figure 6).
All parameters and correlation coefficients (R ) are summarized in Table 3. It can be found that the correlation coefficients of the first-order kinetic model are lower than 0.9, which are less than the second-order kinetic model. Therefore, the adsorption processes was considered to be explained better by the second-order kinetic model. The values of qe
are close to constant with an increase in temperature for adsorption of AFB1 onto ACSH and CCSH. It implies that the temperature does not favor AFB1 adsorption onto ACSH and CCSH and the type of adsorption can be considered as physisorption. The adsorption rate is an important indicator reflecting the properties of adsorbent that is described by the Weber-Morris equation. Generally, the adsorption processes include three stages. The first is considered as a diffusion of adsorbate through the solution to the external surface of
Table 3: Kinetic parameters for AFB1 adsorption onto ACSH and CCSH at different temperatures.
Kinetic models T (K) ACSH CCSH
fcj (min ') qe (^gg-1) R2 fcj (min *) 1e (^gg-1) R2
Pseudo-first-order 301 0.034 35.719 0.886 0.037 492.947 0.899
306 0.035 27.283 0.879 0.041 541.502 0.890
311 0.025 26.528 0.893 0.046 549.035 0.866
k2 (g ^g-' min-1) qe (^gg-1) R2 k2 (g ^g-1 min-1) 1e (^gg-1) R2
Pseudo-second-order 301 0.0038 285.714 0.999 0.00017 981.448 0.998
306 0.0056 277.778 0.999 0.00018 977.279 0.998
311 0.0043 277.767 0.999 0.00021 966.428 0.998
k3 (^gg-1 min-1/2) b R2 k3 (^gg-1 min-1/2) b R2
301 6.317 230.76 0.684 53.534 440.40 0.991
306 5.070 239.59 0.628 52.870 450.65 0.994
311 4.877 236.10 0.808 51.435 469.11 0.999
adsorbent or faster adsorption stage. The second describes the gradual layer adsorption stage, where intraparticle diffusion is rate-controlled. The third stage is the final equilibrium stage [32]. It is important to note that the correlation coefficients are lower than 0.81 for ACSH at different temperatures, which suggest a nonlinear relationship. Hence, the adsorption process of AFB1 onto ACSH is not intraparticle diffusion predominantly and three possible adsorption processes may occur. In contrast, the correlation coefficients are higher than 0.99 for CCSH at different temperatures, indicating that the intraparticle diffusion controls the adsorption process, but the intraparticle diffusion curves do not pass through the origin; it indicates that besides intraparticle diffusion, other process may influence the adsorption rate. Moreover, the values of intercept b give an idea about the boundary layer thickness; the larger the intercept, the greater the boundary layer effect [33]. From Table 3, values ofb of CCSH are greater than that of ACSH. This means that the greater boundary layer effect is in favor of AFB1 adsorption onto CCSH.
3.3. Adsorption Isotherms. Adsorption isothermal reflects the relationship between adsorption capacity and the concentration of solution at a fixed temperature. Analysis of the isotherm data is important to examine the adsorption mode of AFB1 onto ACSH and CCSH, and the results can be used for the design of more efficient materials to bind AFB1. Therefore, two main models of adsorption isotherm were analyzed, one is Langmuir isotherm [34] and the other is Freundlich isotherm [35], which is widely applied in liquidsolid adsorption system.
The Langmuir isotherm theory assumes monolayer coverage of adsorbate over a homogenous adsorbent surface. In a modified form it can also describe a bilayer deposition. Firstly, the adsorption data were fitted to the linear form of Langmuir adsorption model (see (4)) and obtained the Langmuir isotherm by plotting the particular adsorption (Ce/C) against the equilibrium concentration of AFB1 (Ce). Ce is the equilibrium concentration of AFB1 which means the final concentration (^g/L) of AFB1 in the solution after adsorption, Cm is the maximum adsorbance (^g/g), C is the amount of AFB1 being adsorbed by the sorbent at equilibrium
Table 4: Isotherm parameters for AFB1 adsorption onto ACSH and CCSH.
Sample Langmuir Cm K (mgg-1) (Lmg-1) R2 Freundlich Kf J Mr (^gg-1) f R2
ACSH -0.333 -0.284 0.807 0.005 1.483 0.996
CCSH 2.0 10.020 0.882 64.358 0.583 0.992
), and K is a constant related to the adsorption energy. The essential characteristics of the Langmuir isotherm can be expressed in terms of a dimensionless constant separation factor R0 which is given by (5). C0 is the initial AFB1 concentration (^g/mL). When R0 is between 0 and 1, it indicates a favorable AFB1 adsorption, when R0 is higher than 1, it indicates an unfavorable AFB1 adsorption.
Consider
Ce _ 1 , C.
C c...
KC0 + 1
The adsorption data were also fitted to the linear form of Freundlich adsorption model which is shown as
lg C_ lg Kf + nf\g Ce,
where Kj is Freundlich constant related to adsorption intensity and nj is constant as well related to adsorption intensity, C (^g/g) and Ce (^g/L) denote adsorbance of AFB1 and equilibrium concentration of AFB1, respectively. Freundlich equation is an exponential variation in site energies and assumes that the adsorbent has a heterogeneous energy distribution of active sites. Theoretically, the Freundlich model is suitable for use with heterogeneous surfaces and with this expression, an infinite amount of adsorption can occur [36].
The absorption isothermal curves of AFB1 onto CCSH and ACSH are shown in Figure 7, and all isotherm values are summarized in Table 4. For Langmuir isotherm shown
1600 1400 1200 ^ 1000 К 800 U 600 400 200 0
75 100 125 Ce (pg/L)
450 -, 400 350 -300 -250 200 150 100 50 0
150 175 200
0 250 500 750 1000 1250 1500 1750 2000 2250 2500 Ce (^g/L)
Figure 7: Adsorption isotherms for AFB1 onto CCSH (a) and ACSH (b) at 301K.
0.16 -, 0.14 -0.12 -
/L) 0.1
^ 0.08 -(J 0.06 -
0.04 0.02 0
12 1 10
8 6 4 2 0
0 25 50 75 100 125 150 175 200 Ce (те/L)
♦ CCSH
♦ ACSH
1000 1500
Ce (^g/L)
Figure 8: Langmuir isotherm for AFB1 onto CCSH (a) and ACSH (b) at 301K.
in Figure 8, R0 of CCSH is between 0.166 and 0.032, which indicates a favorable adsorption of AFB1 onto CCSH. R0 of ACSH is higher than 1, indicating an unfavorable adsorption. Meanwhile, for Freundlich isotherm shown in Figure 9, nj of ACSH at equilibrium is bigger than 1, as nj is related to adsorption intensity or surface heterogeneity, which ranges between 0 and 1 when a favorable adsorption occurrs. This result indicates that ACSH has a poor adsorption capacity of AFB1 in the solution. Meanwhile, nj of CCSH is smaller than 1, so this adsorbent is favorable for adsorbing AFB1. Comparing the values of R , it is noted that the Freundlich model yielded a much better fit than the Langmuir model for both of ACSH and CCSH. It can be deduced that the adsorptions of AFB1 on the surface of adsorbents are uneven.
3.4. Adsorption Mechanism. All results above indicate that CCSH is a suitable adsorbent for the adsorption of AFB1. In order to study it further, the adsorption mechanism of AFB1 onto CCSH was deduced in the following steps and the description is shown in Figure 10.
Firstly, when CSSH and AFB1 were mixed together in solution, the AFB1 molecules transfer from the bulk solution to the CCSH external surface by diffusing through the boundary layer. At this moment, the adsorption of AFB1 onto CCSH was surface adsorption. The main component of CCSH is tobermorite and an important feature of the structure of tobermorite is the cavity between two adjacent building layers, the "interlayer space," which contain water and Ca + ions [26]. When Ca2+ ions diffuse into the solution, CCSH is electronegative. For AFB1 molecule, the center of positive charges and that of negative charges does not coincide, which belongs to polar molecule (Figure 1). Through the mode of charge adsorption, polar molecules of AFB1 are adsorbed onto CCSH with negative charges, while the Si-OH on the surface of CCSH can also bind AFB1 through the hydrogen bonding interaction [37]. This adsorption process has little influence on the adsorption rate.
The second reactive stage is the gradual layer adsorption stage. With the continuous adsorption reaction, the AFB1 molecules diffuse from the external surface into the interlayer of CCSH. While more and more AFB1 molecules diffuse into
3.4 -, 3.2 -3 -2.8 -2.6 -2.4 -2.2 2
0.75 1
♦ CCSH
2.9 -, 2.7 2.5 2.3
- 2.1 -1.9 -1.7 1.5
1.25 1.5 1.75 2 2.25 2.5 2.6 2.7 2.8 2.9 3 3.1 3.2 3.3 3.4
lg Ce lg Ce
♦ ACSH
(a) (b)
Figure 9: Freundlich isotherm for AFB1 onto CCSH (a) and ACSH (b) at 301K.
Silicate tetrahedron layer Calcium octahedral layer
Interlayer Ca^f- and H2O| AFB1 molecules
The first stage The second stage
Figure 10: Adsorption description of AFB1 onto CCSH.
The last stage
the interlayer, the "pore blockage" effect occurs which results in the decrease of SBET and Vtotai. As the adsorption of AFB1 in the interlayer is uneven, several adsorption modes can occur (Fit to Freundlich). It means monolayer adsorption of AFB1 may occur in some locations of interlayer and multilayer adsorption of AFB1 may occur too in some other locations, and in the same way even zero adsorption may exist somewhere. Due to such special adsorption mode, the pore structure is changed and ink-bottle pores are formed. As the adsorption process of AFB1 onto CCSH is mainly controlled by intraparticle diffusion, most AFB1 are adsorbed at this stage with the longest reaction time, which controls the adsorption rate (Fit to intraparticle diffusion model). Meanwhile, it is seen that the concentrations of AFB1 and CCSH can also affect the adsorption rate (fit to the pseudo-second-order kinetic model).
The last stage is the final equilibrium stage. The diffusion of every substance in the system achieves a state of dynamic equilibrium, such as Ca2+ ions, AFB1 molecules, particles of adsorbent, and water molecules. At this stage, the adsorption reaction tends to be stable and the adsorbance of AFB1 on CCSH reaches the maximum value.
4. Conclusions
The results present in this paper demonstrate the characteristics of CSH and their performance in the adsorption of AFB1
from aqueous solution. It shows that both ACSH and CCSH can adsorb AFB1, but CCSH is more effective in the adsorption of AFB1. The CSH was characterized by several methods; from the analysis of results, it indicates that a regular layered structure is beneficial for the adsorption of AFB1. CCSH consisting of crystallized tobermorite mainly adsorbs AFB1 into its interlayer, resulting in a higher adsorption of AFB1 and the decrease of SBET remarkably; it is also the reason for the change of pore structure. For CSH, kinetic studies indicates that the adsorption follows the pseudo-second-order kinetic model (R2 > 0.99) and the adsorbance of AFB1 is near constant with an increase in temperature. The adsorption process of AFB1 onto CCSH is intraparticle diffusion (R > 0.99) predominantly but not for ACSH (R2 < 0.81). The data of equilibrium adsorption were fitted to Langmuir and Freundlich equations; Freundlich isotherm model correlates the experimental data better than Langmuir, which implies that adsorptions of AFB1 on the surface of CSH are uneven. Summary analysis of all results, the adsorption mechanism of AFB1 onto CCSH was deduced by dividing into three stages. As a result, it can be concluded that CCSH has a great potential to be used as an adsorbent for the removal of AFB1 from aqueous solutions, which is safe, easy-made, and cost effective.
Conflict of Interests
The authors declare no conflict of interests.
Acknowledgment
The study was supported by the Fundamental Research Funds
for the Central Universities (CDJZR12130052).
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