Scholarly article on topic 'Biomethane production from vinasse in upflow anaerobic sludge blanket reactors inoculated with granular sludge'

Biomethane production from vinasse in upflow anaerobic sludge blanket reactors inoculated with granular sludge Academic research paper on "Chemical engineering"

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Academic research paper on topic "Biomethane production from vinasse in upflow anaerobic sludge blanket reactors inoculated with granular sludge"

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BRAZILIAN JOURNAL OF MICROBIOLOGY

SOCIEDADE BRASILEIRA DE MICROBIOLOGIA

http://www.bjmicrobiol.com.br/

Environmental Microbiology

Biomethane production from vinasse in UASB reactors inoculated with granular sludge

Valciney Gomes de Barrosa'b'*, Rose Maria dudaa,c, Roberto Alves de Oliveiraa,b

a Universidade Estadual Paulista, Faculdade de Ciencias Agrárias e Veterinárias, UNESP, Campus de Jaboticabal, SP, Brazil b Laboratorio de Saneamento Ambiental, Departamento de Engenharia Rural, UNESP, Campus de Jaboticabal, SP, Brazil c Faculdade de Tecnologia de Jaboticabal (Fatec), Jaboticabal, SP, Brazil

article info abstract

Article history: The main objective of this study was to evaluate the anaerobic conversion of vinasse into

Received 12 October 2015 biomethane with gradual increase in organic load rate (OLR) in two upflow anaerobic sludge

Accepted 8 January 2016 blanket (UASB) reactors, R1 and R2, with volumes of 40.5 and 21.5 L in the mesophilic

Available online xxx temperature range. The UASB reactors were operated for 230 days with a hydraulic detec-

Associate Editor: Adalberto Pessoa tion time (HDT) of 2.8 d (R1) and 2.8-1.8 d (R2). The OLR values applied in the reactors

Junior were 0.2-7.5 gtotalCOD (Ld)-1 in R1 and 0.2-11.5 gtotalCOD (Ld)-1 in R2. The average totalCOD

__removal efficiencies ranged from 49% to 82% and the average conversion efficiencies of the

Keyu)0rds:removed totaiCOD into methane were 48-58% in R1 and 39-65% in R2. The effluent recircula-Biogas tion was used for an OLR above 6 gtotalCOD (L d)-1 in R1 and 8 gtotalCOD (L d)-1 in R2 and was

Mesophilic anaerobic treatment able to maintain the pH of the influent in R1 and R2 in the range from 6.5 to 6.8. However, this

Methane caused a decrease for 53-39% in the conversion efficiency of the removed COD into methane

Organic load rate in R2 because of the increase in the recalcitrant COD in the influent. The largest methane

Startup yield values were 0.181 and 0.185 (L) CH4 (gtotalCOD removed)-1 in R1 and R2, respectively.

These values were attained after 140 days of operation with an OLR of 5.0-7.5 gtotalCOD (L d)-1 and totalCOD removal efficiencies around 70 and 80%.

© 2016 Published by Elsevier Editora Ltda. on behalf of Sociedade Brasileira de Microbiologia. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Introduction

Ethanol is the world's most widely used biofuel.1 The global production of ethanol in 2011 was 86.1 billion liters, of which the US and Brazil contributed 62.7% and 24.4%, respectively.2

Vinasse is the final residue obtained during ethanol pro- 25

duction by fermentation of sugarcane.3 For one liter of ethanol 26

produced from sugarcane is estimated to output 8-18 L of 27

vinasse.4 The vinasse leaves the distillation column with 28

a temperature of about 90 ° C and pH between 3 and 4. It 29 constitutes 94-97% water, Mg2+, Ca2+, K+, melanoidins, and

* Corresponding author. E-mail: valcineygb@yahoo.com.br (V.G. de Barros). http://dx.doi.Org/10.1016/j.bjm.2016.04.021

1517-8382/© 2016 Published by Elsevier Editora Ltda. on behalf of Sociedade Brasileira de Microbiologia. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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residual amounts of sugar, alcohol, and volatile components such as chloroform, pentachlorophenol, phenol, and methylene chloride, and the amount of these substances depends on the feedstock and the process of ethanol production.4,5 The España-Gamboa et al.,6 reported the presence of antibacterial components and heavy metals in vinasse. Approximately 75% of suspended solids present in vinasse are organic and biodegradable,7 which provides a high chemical and biochemical oxygen demand (COD and BOD) of up to 100gO2L-1 and 60 gO2 L-1, respectively.3,8

In Brazil, vinasse is mainly used in fertigation of sugarcane in adjacent areas of ethanol production industries because of its high organic matter and nutrient contents.6 However, studies suggest that the application of vinasse indiscriminately in soil could contaminate surface water and groundwater.9

In addition, the anaerobic digestion of vinasse may be utilized for stabilization of organic material and methane production, which can be used to produce energy required for drying yeast in distillery.8 During the anaerobic digestion of vinasse, most of the organic matter is removed, leaving the recalcitrant organic compounds and most of the nutrients in the effluent.5

The higher temperature of vinasse promotes thermophilic anaerobic digestion. However, in some industries, the systems that utilize the thermal energy of vinasse are currently being installed. This makes the topic of mesophilic anaerobic digestion interesting,10 and studies have shown its advantage over thermophilic anaerobic digestion in terms of impartin greater stability.7

Currently, the upflow anaerobic sludge blanket (UASB) reactor is the most widely used reactor for the treatment of vinasse obtained from the ethanol industry.11 Low sludge production and the conversion of approximately 50% of the total chemical oxygen demand (totalCOD) of vinasse to biogas have couples of advantages of using the UASB reactor.6 The design of the reactor is quite simple and does not

require sophisticated equipment.12 This enables the use of UASB technology for treating industrial waste such as vinasse.

In the UASB reactors, the microorganisms are mainly grouped into granules and flocs formed by self-aggregation of bacteria and archaea. These formations greatly depend on the upflow and composition of wastewater. The granules are compact clusters that possess high specific methanogenic activity and sedimentation. They accumulate in large quantities in the UASB reactor.12

For efficient methane production from vinasse, strategies need to be developed for startup and maintenance of the anaerobic microbiota. High concentrations of phenolic compounds, such as melanoidins, present in the vinasse,9 heavy metals, and antibacterials used in the treatment of yeast may decrease and even inhibit the microbial activity.13

The startup time of the anaerobic reactor without adapted sludge inoculum can be up to 40% higher than that of a reactor with the use of adapted sludge.6

Within this context, this study aims to assess the startup and stabilization of the anaerobic conversion of vinasse to methane with gradual increase of organic load rate in UASB reactors.

Materials and methods

g Reactor configuration

The experimental unit consisted of two bench-scale UASB reactors (R1 and R2) of capacity 40.5 L and 21.5 L, respectively (Figure 1). The reactors R1 and R2 have five and four sludge collection points, respectively, distributed along the height of the reactors (97.8 and 108.5 cm, respectively, Figure 1). In R1, the sludge collection points P1, P2, P3, P4, and P5 are located at 5, 23.5, 37.4, 51.9, and 65.7 cm from the base of the reactor. In

80 81 82

UASB R1

UASB R2

Figure 1 - Schematic representation of the treatment system with upflow anaerobic sludge blanket (UASB) reactors (R1 and R2).

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OLR R1 (Time)

OLR R2 (Time)

adjustment

(Time)

pH R2 adjustment (Time)

0.2 to 2.5 (115 d) 2.5 to 5.0 (32 d) 5.0 to 7.5 (39 d) 7.5 (43d)

0.2 to 2.5 (115 d) 2.5 to 5.0 (32 d) 5.0 to 11.5 (25 d) 7.5 to 11.5 (21 d) 11.5 (36 d)

NaOH (158 d) Recirc ( 7 ulation d)

NaOH (172 d) Recirculation (57 d)

0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230

Operation time (d)

Figure 2 - Schematic diagram with the operating conditions of organic load rate (OLR in g total COD (Ld)-1) and pH adjustment in UASB reactors (R1 and R2) for vinasse treatment.

R2, the sludge collection points P1, P2, P3, and P4 are located at 5, 25, 40.9, and 71.8 cm from the base of the reactor.

Inoculum and influent characteristics

For the UASB reactor startup, the inoculum was granulated sludge from the UASB reactor used to treat swine wastewater. The inoculum sludge had total (TS) and volatile (VS) solid concentrations of 45.6 gL-1 and 30.4 gL-1, respectively. The volume of the sludge used was sufficient to occupy 30% of the volume of each reactor.

The influent used to feed the UASB reactors was obtained from in natura vinasse from a sugarcane plant in Ribeirao Preto, SP. The vinasse was collected weekly, from April to December 2012, after distillation in the wine columns, cooled down, and kept chilled.

The concentrations of totalCOD, TS, VS, total N, total P, K, Ca, Mg, Na, Fe, Mn, Zn, and Cu were determined for characterization of vinasse.14 The concentrations of totalCOD, ST, and SV were 45,000; 41,300 and 31,800mgL-1, respectively. The Kjeldahl N and total P, K, Ca, Mg, Na, Fe, Mn, Zn, and Cu concentration were 470, 170, 88, 3.2, 1.4, 20.4, 24.6, 2.44, 0.78, and 0.21 mgL-1, respectively.

A totaiCOD of about 45,000mgL-1 was necessary to dilute the vinasse and gradually increase the totalCOD of the influent, consequently increasing the organic load rate (OLR) in the UASB reactors. Initially, vinasse was diluted with water, and subsequently, the effluent from the UASB reactors was recirculated. Recirculation of the effluent allows limited use of dilution water and alkalizing as well as reuse of nutrients remaining in the effluent.

Operating conditions of the reactors

The R1 was operated with a hydraulic retention time (HDT) of 2.8 d, while R2 was first operated with an HDT of 2.8 d for 219 days and then decreased to 1.8 d. The HDT was decreased to obtain a gradual increase in OLR. The OLR was calculated by dividing the totalCOD of the influent by HDT.

The upflow velocity in the reactors R1 and R2 were similar, 0.019 mh-1 and 0.018 mh-1, respectively. The surface loading rates in the settlers of the UASB reactors were 0.011 mh-1 (R1) and 0.014mh-1 (R2). With the decrease in HDT for R2,

the upflow velocity and the surface loading rate in the settler of the UASB reactor increased to 0.028mh-1 and 0.022mh-1, respectively.

Although the reactors had different dimensions, they were assumed to be identical, and the same operational conditions (HDT, OLR, alkalizing, and recirculation) were applied. This consideration allowed us to assess the effects of the variables by maintaining one reactor in a normal stable condition while the other was being subjected to new operational conditions.

The OLR was increased from 0.2 to 7.5 gtotalCOD (Ld)-1 in R1 and from 0.2 to 11.5gtotalCOD (Ld)-1 in R2 (Figure 2). The OLR was gradually increased to adapt the inoculum sludge and to obtain stability with higher OLR.

The mean pH value of the in natura vinasse was 4.5. Therefore, it was necessary to correct the pH of the influent to approximately 7.0. Until 158 and 172 days of operation of R1 and R2, respectively, pH of the influent was corrected by adding a solution of 12 M NaOH. After this period, use of NaOH was discontinued and the effluent was recirculated, utilizing the alkalinity generated in the reactors for pH correction (Figure 2). Effluent recirculation in R1 and R2 was started with an OLR of 6 and 8gtotalCOD (Ld)-1, respectively.

The totalCOD, Kjeldahl nitrogen (KN), and totalP found in in natura vinasse were 45,000; 470mgL-1 and 62mgL-1, respectively. These values did not correspond to the recommended minimum proportion of COD:N:P = 350:5:1 for proper microbial growth.15 For supplemental phosphorus and nitrogen, potassium phosphate monobasic (KH2PO4) and urea (CH4N2O) were added to vinasse.16

Analytical methods

Table 1 shows the physical examinations and organic and inorganic constituents determination methods adopted for the samples of influents, effluents, sludge, and biogas of the reactors. The frequency and bibliographic references of the methodologies used are also listed in the table.The air temperature near R1 and R2 was measured daily with a thermometer, and the mean values ranged from 20 °C to 30 °C. Therefore, the reactors were operated predominantly in the mesophilic temperature range.The daily volume of methane produced in the

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Table 1 - Determination and examination, frequency

and bibliographic reference of the methodologies used

for influent, effluent, sludge, and biogas.

Examination and Frequency Bibliographic

determination

Influent and effluent Reference

pH Twice a week (Method: 4500 - B)14

Total (totalCOD), dissolved Twice a week (Method: 5220 - B)14

(dissCOD), and

suspended (ssCOD)

chemical oxygen

demand

total (TA), partial (PA), Twice a week 14,17

and intermediary (IA)

alkalinity

Total (TSS), volatile (VSS), Twice a week (Method: 2540 - C e

and fixed (FSS) suspend 2540 - E)14

solids

Total volatile acids (TVA) Twice a week 18

Kjeldahl nitrogen (KN) Twice a week (Method: 4500-N-C)14

Total phosphorus (totalP) Twice a week (Method: 4500-P-C)14

Sludge

Total solids (TS) and Biweekly (Method 2540 - B

volatile solids (VS) and 2540 - E)14

Biogas

Production Daily (Method: gasometer)19

Composition Weekly 14 (Method: gas chromatography)

177 reactors was corrected to standard temperature and pressure

178 (0 °C and 1atm) (STP).

Results and discussion

pH, alkalinity, and total volatile acids

In the first 158 (R1) and 172 (R2) days of the operation, the pH values of the influent when OLR was 0.2-7.5 gtotaiCOD (Ld)-1 were between 6.5 and 7.0 (Figure 3) because the influent was corrected with NaOH solution. The ratio of total alkalinity (TA)/COD in the influent of the reactor was 0.07-0.11. After this period and when recirculation of the effluent began, the pH values of the influent increased to 7.0-7.5 in R1 and 6.0-7.0 in R2 (Figure 3). This increase was due to the increase in the TA/COD ratio in the influent to 1.5 because of the recirculation. The TA/COD ratio greater than 0.2 in the influent suggests that the alkalinity in the reactor is sufficient to be operated with stability.20

The effluent pH in both the reactors ranged from 7.0 to 8.0. With the recirculation of the effluent, the pH remained steady around 7.8 in R1, which was operated with a smaller OLR (Figure 3). These values are close to the range 6.7-7.8 considered ideal for the development of methanogenic archaea.21

The average concentrations of TA in the effluents of both reactors increased from 532 and 558 mgL-1 to approximately 4280 and 3394mgL-1, respectively, with the increase in OLR from 0.2 to 7.5 gtotalCOD (Ld)-1 (Table 2 and Figure 4).

In R2, after 193 days of operation, with an OLR of 11.5gtotalCOD (Ld)-1 and HDT of 2.8d, the influent pH decreased to below 6.0. Approximately 40% of the effluent was being recirculated to correct the pH. Souza et al.16 used at least 50% effluent recirculation rate to maintain an influent pH of 7.0 in a thermophilic UASB treatment of vinasse. Therefore, after 218 days, HDT in R2 was reduced from 2.8 to 1.8 d, which increased the volume of the recirculated effluent and thus contributed to pH correction to approximately 7.0 and to subsequent TA increase (Figures 3 and 4).

The average concentrations of total volatile acids (TVA) in the influent and effluent of the reactors increased with gradual increase in OLR. Maximum TVA was 1728mgL-1 with an OLR of 7.5gtotalCOD (Ld)-1 in R1 and 2722mgL-1 with an OLR of 11.5gtotalCOD (Ld)-1 in R2. The accumulation of TVA in the treatment of vinasse was mentioned by Souza et al.16 and Espinosa et al.22 The typical reactor response to rapid changes in OLR could lead to massive TVA concentrations, drop in pH, and consequent failure of the process.23

With the methods used to control the influent pH, the average intermediate alkalinity (IA)/partial alkalinity (PA) ratios in the effluent were low (0.18-0.35) in R1 and higher (0.23-0.86) in R2 because of a higher OLR of 11.5 gtotalCOD (Ld)-1 applied (Table 2). According to Ripley et al.,24 an IA/PA ratio of above 0.3 indicates the occurrence of disorders in the anaerobic digestion process.

The average TVA/TA ratios in the effluents were 0.13-0.31 in R1 and 0.12-0.50 in R2 (Table 2) with increased OLR. The largest TVA/TA ratios were obtained for an OLR of 11.5gCOD (Ld)-1 with increasing TVA concentration from 2596 to 4663mgL-1. This is because of the decrease in the total COD removal efficiency from 82% to 60%.

The TVA/TA ratio of above 0.8 may inhibit methanogenic archaea, that of 0.3-0.4 indicates an unstable system, and a ratio of 0.1-0.2 is appropriate.25 Following this finding, instabilities existed only when R2 was operated with an OLR of 11.5 gtotalCOD (Ld)-1, and stable operation of both UASB reactors was possible with an OLR of up to 7.5 gtotalCOD (Ld)-1.

COD and suspended solids

The values of totalCOD of the influent were 1866-21,971 mgL-1 in R1 and 1866-28,543mgL-1 in R2 (Table 3) with the gradual increase in OLR. The average values of dissolved COD (dissCOD) were 84-89% of totalCOD, indicating that most of the organic matter of the influent can be found mainly soluble.

The concentrations of total and volatile suspended solids (TSS and VSS) in the influent were 117-1910mgL-1 and 96-1556 mgL-1, respectively (Table 3). VSS was 69-85% of TSS, thus indicating that organic suspended solids were predominant.

The maximum average efficiencies of totalCOD and dissCOD removal in the UASB reactors of 81% and 82%, respectively, were achieved with an OLR of 2.5-5.0gtotalCOD (Ld)-1 in R1 and R2. With the increase in average OLR from 5.0 to 7.5gtotalCOD (Ld)-1 in R1 and R2, and 7.5-11.5totalCOD (L d)-1 in R2, the removal efficiencies of totalCOD and dissCOD remained similar, 70-82%, respectively (Table 4 and Figure 5). Therefore, the strategy used (inoculated with granular sludge,

201 202

210 211 212

220 221 222

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Recirculation

. /»-A /\/'"'"\ /••" .....

\........................./ ..." \ / >.-.-

......................J

7 17 27 38 52 62 73 82 94 108 118 129 139 151 161 172 181 193 202 216 227

Operation Time (d)

--Influent R1 — Effluent R1

8.50 - Recirculation

8.00 -7.50 -7.00 - A ■» >1-1 iVls > ■ ■ s ' V

6.50 - \ AM .. /'""'

............\ y'"«-!'"-

6.00 -

7 17 27 38 52 62 73 82 94 108 118 129 139 151 161 172 181 193 202 216 227

Operation Time (d)

---Influent R2 —»— Effluent R2

Figure 3 - pH values of the influents and effluents of UASB reactors (R1 and R2) for vinasse treatment.

Table 2 - Average values of the concentrations of total alkalinity (TA), total volatile acids (TVA) of the influents and

effluents; ratios IA/PA and TVA/TA of the effluents; and OLR applied during the operation of the UASB reactors (R1 and

R2) for vinasse treatment.

Attributes R1 R2

OLR (gtotaiCOD (Ld)-1) 0.2-2.5 2.5-5.0 5.0-7.5 7.5 0.2-2.5 2.5-5.0 5.0-7.5 7.5-11.5 11.5

Operation days (0-115) (116-147) (148-186) (187-229) (0-115) (116-147) (148-172) (173-193) (193-229)

Influent 6.94 6.51 6.80 6.80 6.94 6.51 6.80 6.80 6.48

vc 2.7 0.3 4.4 2.8 2.7 0.3 4.4 2.9 8.8

Effluent 7.41 7.88 7.89 7.82 7.52 7.88 7.93 7.98 7.71

vc 4.5 2.8 1.8 7.8 4.5 2.4 1.4 1.0 3.3

TA (mgL-1 CaCO3)

Influent 216 781 2349 4828 216 781 873 2284 4386

vc 38 31 46 17 38 31 25 40 28

Effluent 532 2529 4280 6100 558 2517 3394 4090 6288

vc 64 20 20 29 57 34 24 18 20

TVA (mgL-1 CH3COOH)

Influent 158 943 1599 3050 158 943 1262 2596 4663

vc 77 44 37 6 78 44 30 11 10

Effluent 60 388 623 1728 61 328 499 454 2722

vc 48 67 23 23 43 56 15 16 48

Effluent 0.32 0.18 0.24 0.35 0.32 0.16 0.36 0.23 0.86

vc 76 28 16 26 69 31 30 33 70

TVA/TA

Effluent 0.13 0.15 0.14 0.31 0.12 0.13 0.15 0.11 0.50

vc 34 47 13 45 36 39 15 8 58

OLR, organic load rate; IA, intermediary alkalinity; PA, partial alkalinity; vc, variation coefficient (%).

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10000 -8000 -6000 -4000 -2000 0

6 4 2 0

7 17 27 38 52 62 73 82 94 108 118 129 139 151 161 172 181 193 202 216 227

Operation time (d)

—■—Influent R1 —•—Effluent R1 -OLR

—■—Influent R2--Effluent R2 -OLR

Figure 4 - Concentrations of total alkalinity (TA) as a function of the organic load rate (OLR) applied in UASB reactors (R1 and R2) for vinasse treatment.

Table 3 - Average values of total COD, dissCOD, TSS, and VSS of the influents and effluents and the OLR applied during the

operation of UASB reactors (R1 and R2) for vinasse treatment.

Attributes R1 R2

OLR (gtotalCOD (Ld)-1) 0.2-2.5 2.5-5.0 5.0-7.5 7.5 0.2-2.5 2.5-5.0 5.0-7.5 7.5-11.5 11.5

Operation days (0-115) (116-147) (148-186) (187-229) (0-115) (116-147) (148-172) (173-193) (193-229)

totajCODa

Influent 1866 10,377 17,554 21,971 1866 10,377 16,239 24,800 28,543

vc 94 22 13 3 94 22 9 15 17

Effluent 415 2037 5637 10,904 378 1888 3748 4404 10,540

vc 59 40 37 10 58 45 14 7 11

toMCODa

Influent 1568 8897 15,344 18,809 1568 8897 14,569 21,430 24,103

vc 91 27 13 7 91 27 10 13 19

Effluent 335 1705 4852 9689 308 1579 3110 3765 9122

vc 75 33 37 13 74 34 13 10 16

Influent 139 480 1001 1869 117 480 775 1582 1910

vc 129 36 53 11 89 36 50 51 17

Effluent 30 201 731 1150 28 143 427 668 1100

vc 109 46 48 9 121 50 32 35 19

Influent 96 355 785 1385 88 355 663 1182 1556

vc 113 38 49 13 102 38 51 41 20

Effluent 19 137 428 696 14 102 285 339 706

vc 98 48 38 16 69 58 40 45 24

a Unit:mgL-1.

OLR, organic load rate; totaiCOD, total chemical oxygen demand; dissCOD, dissolved chemical oxygen demand; TSS, total suspended solids;

VSS, volatile suspended solids; vc, variation coefficient (%).

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Table 4 - Average values of OLR and the removal efficiencies (in %) of total COD, diss COD, TSS, and VSS during the operation of the UASB reactors (R1 and R2) for vinasse treatment.

Attributes R1 R2

OLR (gtotalCOD (Ld)-1) 0.2-2.5 2.5-5.0 5.0-7.5 7.5 0.2 -2.5 2.5-5.0 5.0-7.5 7.5-11.5 11.5

Operation days (0-115) (116-147) (148-186) (187-229) (0- 115) (116-147) (148-172) (173-193) (193-229)

totalCOD 67 81 67 49 69 82 77 82 60

vc 32 5 13 10 30 5 4 4 15

diss.COD 72 81 68 47 73 82 78 82 60

vc 26 3 13 13 22 3 5 3 17

TSS 64 59 24 38 65 73 46 54 41

vc 40 38 80 25 39 31 122 20 24

VSS 71 50 41 49 65 60 54 70 54

vc 44 85 51 10 48 72 21 12 12

OLR, organic load rate; totaiCOD, total chemical oxygen demand; dissCOD, dissolved chemical oxygen demand; TSS, total suspended solids; VSS, volatile suspended solids; vc, variation coefficient (%).

pH correction with NaOH and application of increasing OLR) allowed the startup and stabilization of COD removal in UASB reactor with OLR up to 11.5 gtotalCOD (Ld)-1.

The removal efficiencies of totaiCOD decreased to approximately 50% and 60% with the recirculation of the effluent when OLR of 7.5 and 11.5gtotalCOD (Ld)-1 was applied in R1 and R2, respectively. This was due to the increase in the amount of compounds that cannot be easily degraded with the subsequent recirculation of the effluent.

For a successful startup of the anaerobic treatment of vinasse from wine distillery, Wolmarans and Villers26 recommended the UASB reactor to be operated at an OLR of 4.0-8.0gtotalCOD (Ld)-1 until 90% totalCOD removal is

achieved, which will initiate the gradual increase of OLR. This result was similar to that of our work. However, other studies on sugarcane vinasse treatment in UASB reactors attained successful with COD removal at an efficiency below 90%,16,27 which resulted in an OLR up to 30gtotalCOD (Ld)-1.16 This confirms that it is possible to increase the OLR, but the COD removal decreases, as occurred in UASB reactors (R1 and R2) with OLR above 7.5 gtotalCOD (Ld)-1.

The average values of TSS removal efficiency decreased from 64% to 38% in R1 and from 65% to 41% in R2 when OLR was increased from 0.2 to 7.5 gtotalCOD (Ld)-1 in R1 and from 0.2 to 11.5gtotalCOD (Ld)-1 in R2 (Table 4). This decrease in TSS removal efficiency is due to wash out of the sludge in the reactors. This issue was solved by discarding 10% of the

280 281 282

-PPPI-F

52 62 73 82 94 108 118 129 139 151 161 172 181 193 202 214 223 Operation time (d)

7 17 27 38 52 62 73 82

94 108 118 129 139 151 161 172 181 193 202 214 223 Operation time (d)

Figure 5 - Removal efficiency of the total chemical oxygen demand (totaiCOD) as a function of the organic load rate (OLR) applied in the UASB reactors (R1 and R2) for vinasse treatment.

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7.5 g total COD/L d «5.0 a 7.5 g total COD/L d 2.5 a 5.0 g total COD/L d 0 a 2.5 g total COD/L d

40 TS (g/L)

TS (g/L)

Figure 6 - Concentration of total solids (TS) in the sludge collected at the points shown in Figure 1 as a function of the organic load rate (OLR) applied in the UASB reactors (R1 and R2) treating vinasse. (A) R1 and (B) R2.

287 volume of sludge blanket of both reactors with an OLR of

288 5.0-7.5 gtotalCOD(Ld)-1.

289 Total and volatile solids in the sludge

290 TS concentration in the sludge of the UASB reactors (R1 and

291 R2) increased with OLR (Figure 6A and B), indicating that there

292 was an increase in the sludge blanket of the reactors.

293 In R1, TS concentration in the sludge increased from 49

294 to 75 gL-1, 41 to 72 gL-1, 31 to 55 gL-1, 5 to 28 gL-1 and 5 to

295 30 gL-1 at collection points P1, P2, P3, P4, and P5, respectively,

296 and with an OLR of 0.2-7.5 gtotalCOD (L d)-1(Figure 6A).

297 In R2, TS concentration in the sludge increased from 45 to

298 70 gL-1, 42 to 68 gL-1, 31 to 45 gL-1 and 5 to 25 gL-1 at collec-

299 tion points P1, P2, P3, and P4, respectively, and with an OLR of

300 0.2-11.5 gtotalCOD (Ld)-1 (Figure 6B).

301 The decrease in TS concentration in the sludge from both

302 reactors at collection points P3 and P4 is due to the disposal of

303 excess sludge (10% of the volume of the sludge blanket). This

304 prevented VSS wash out with the effluent and was performed

305 with an OLR of 5.0-7.5 gtotalCOD (Ld)-1.

The ratio of volatile and total solids (VS/TS) in the sludge 306

from the UASB reactors ranged from 0.54 to 0.76 (Table 5). 307

These values indicate the predominance of organic matter 308

in the sludge, and thus the presence of microorganisms, as 309

confirmed by intensive conversion of totalCOD removed into 310

methane (Table 6). 311

According to Brazilian legislation (Resolution n° 37528), the 312

sewage sludge or derived product is considered stable for agri- 313

culture use if VS/TS <0.70. Therefore, it was observed that the 314

sludge has been stabilized, especially in the top of the sludge 315

blanket (collection points, P3, P4, and P5 from R1 and P3 and 316

P4 from R2). Thus, when necessary, sludge disposal should be 317

performed from P3 because at this point VS/TS < 0.7. 318

The organic load in the sludge (OLS) ranged from 0.16 319

to 0.42 gtotalCOD (gVSd)-1 in R1 and 0.15 to 0.67 gtotalCOD 320

(gVS d)-1 in R2, with an increase in OLR (Table 5). The recom- 321

mendation of Chernicharo12 was followed. The OLS during the 322

startup of the UASB reactors was maintained between 0.05 and 323

0.15 gtotalCOD (gVS d)-1. It was gradually increased to a value 324

lower than 2.0 gtotalCOD (gVS d)-1 depending on the removal 325

efficiencies. 326

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Table 5 - Average values of the ratio of VS/TS in the sludge, organic load in the sludge (OLS) and OLR during the

operation of the UASB reactors (R1 and R2) for vinasse treatment.

Attributes R1 R2

OLR (gtotalCOD (Ld)-1) 0.2-2.5 2.5-5.0 5.0-7.5 7.5 0.2-2.5 2.5-5.0 5.0-7.5 7.5-11.5 11.5

Operation days (0-115) (116-147) (148-186) (187-229) (0-115) (116-147) (148-172) (173-193) (193-229)

P1 VS/TS 0.67 0.74 0.68 0.75 0.65 0.75 0.73 0.69 0.74

vc 19 2 14 4 20 1 0 2 2

VS/TS 0.58 0.76 0.68 0.71 0.56 0.71 0.74 0.66 0.76

vc 13 0 15 2 1 6 0 5 1

VS/TS 0.63 0.73 0.68 0.69 0.58 0.69 0.74 0.61 0.65

vc 1 3 13 11 3 8 0 7 0

VS/TS 0.58 0.64 0.68 0.66 0.57 0.55 0.63 0.54 0.63

vc 18 18 4 11 24 6 0 2 4

VS/TS 0.58 0.56 0.59 0.53 - - - - -

vc 14 4 7 28 - - - - -

OLS (gtotCOD (gVSd)-1) 0.16 0.27 0.38 0.42 0.15 0.27 0.34 0.68 0.67

OLR, organic load rate; OLS, organic load in the sludge; VS, volatile solids; TS, total solids; vc, variation coefficient (%). P1, point 1 (bottom); P2,

point 2; P3, point 3; P4, point 4; P5, point 5 (top), as shown in Fig ure 1.

327 Methane

328 Methane percentage in the biogas decreased from 83% to 69%

329 and from 85% to 64% in R1 and R2, respectively, when OLR

330 was increased (Table 6). However, the volumetric methane

331 production reached up to 0.8 L CH4 (L reactor d)-1 in R1 and

332 1.3 L CH4 (L reactor d)-1 in R2 when higher OLR values of 7.5

333 and 11.5 gtotajCOD (L d)-1 were applied, respectively (Figure 7).

334 The highest average values of the volumetric methane pro-

335 duction were 0.597 and 0.989 L CH4 (L d)-1 with OLR values of

336 5.0-7.5 gtotaiCOD (Ld)-1 in R1 and 7.5-11.5 gtotaiCOD (Ld)-1 in

337 R2.

The specific methane production increased from 0.133 to 0.181 L CH4 (gtotaiCOD removed)-1 in R1 and from 0.145 to 0.185 L CH4 (gtotalCOD removed)-1 in R2 with the application of an OLR of 0.2-7.5gtotalCOD (Ld)-1. With higher values of OLR and effluent recirculation, the average values of specific methane production decreased to 0.172 and 0.115 L CH4 (gtotalCOD removed)-1 in R1 and R2, respectively (Table 6). The methane yield was below the theoretical value of 0.35 L CH4 (gCOD removed)-1 calculated stoichiometrically. The methane yield obtained by Souza et al.,16 was 0.37 L CH4 (gCOD removed)-1 with an OLR of 26.5 g COD (Ld)-1 and that obtained by España-Gamboa et al.,6 was 0.26L CH4 (gCOD

Table 6 - Average values of OLR, volumetric and specific methane production, and mass balance for conversion of removed COD into methane during operation of UASB reactors (R1 and R2) for vinasse treatment.

Attributes UASB reactor

OLR (gtotalCOD (Ld)-1)

Volumetric ethane production* (LCH4 (Ld)-1)

Specific ethane production* (LCH4 (gtotalCOD removed)-1)

Mass balance (removed COD converted into CH4)*

vc vc (%)

0.2-2.5 0.087 80 0.133 48 58

2.5-5.0 0.440 43 0.175 30 51

5.0-7.5 0.597 18 0.181 20 48

7.5 0.554 4 0.172 35 48

0.0-2.5 0.120 57 0.145 33 65

2.5-5.0 0.550 34 0.179 22 52

5.0-7.5 0.829 21 0.185 14 53

7.5-11.5 0.989 15 0.138 25 39

11.5 0.938 22 0.115 30 42

Values adjusted for standard temperature and pressure (STP) (0°C and 1 atm).

OLR, organic load rate; totalCOD, total chemical oxygen demand; vc, variation coefficient (%).

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c 1.0

£ o 0.8

o c o 0.6

o 0.4

■ Ârfï'

¡M Jà'M-'W

5 47 68 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 Operation time (days)

R1 ---R2

Figure 7 - Volumetric methane production during operation of UASB reactors (R1 and R2) for vinasse treatment.

350 removed)-1 with an OLR of 17.0g COD (Ld)-1 in the vinasse

351 treatment in thermophilic UASB reactors. These results sug-

352 gest that the vinasse anaerobic treatment conducted in the

353 thermophilic phase allows application of larger OLR and yields

354 higher specific methane production.

355 The average conversion rates of totalCOD removed into

356 methane in R1 and R2 were 48-58% and 39-65%, respectively

357 (Table 6). These values are higher by more than 50% compared

358 with those observed by Harada et al.,27

359 Assuming that 10% of removed COD was converted in the

360 sludge, as indicated by Chernicharo,12 methane loss of 25%

361 and 51% was found in R1 and R2. A significant portion of gases

362 generated in the anaerobic treatment can remain dissolved

363 in the liquid and be expelled out with the treated effluent.

The methane loss in the effluent from the UASB reactors can 364

vary from 20% to 50%.21 Therefore, the values assigned to 365

the sludge production and methane losses in the effluent can 366

result in up to 30-60% of COD removal. These values are within 367

the range quoted for R1 and R2. 368

Nitrogen and phosphorus 369

The average concentration of totalP and KN in the influent 370

increased from 56 to 476mgL-1 and from 18 to 63mgL-1, 371

respectively, due to the nutrient supplementation and the 372

increase of OLR in R1 and R2 (Table 7). Supplementation of 373

nitrogen, phosphorus, and potassium can reduce the effects of 374

possible shock loads and prevent flotation of granules in UASB 375

Table 7 - Average concentrations (mgL-1) of Kjeldahl nitrogen (KN), total phosphorus (totaiP) and potassium (K) in the influents and effluents; removal efficiency (E in %); and OLR during operation of UASB reactors (R1 and R2) for vinasse treatment.

Attributes

OLR (gtotalCOD (Ld)-1) 0.2-2.5 2.5-5.0

5.0-7.5

0.2-2.5

2.5-5.0

5.0-7.5

7.5-11.5

Operation days

KN Influent vc

Effluent

total?

Influent vc

Effluent

(0-115) (116-147) (148-186) (187-229) (0-115) (116-147) (148-172) (173-193) (193-229)

56 56 44 39 33 54

18 26 10 96 61 66

28 99 33 40 60

52 27 22 5

30 134

345 48 280 53

37 16 47 41 75

470 22 365 20 27 53

52 49 8

43 58 43

56 56 40

52 27 52 7

79 12 59 22 41 72

56 43 28 53 48 59

63 48 48 45 38 74

Influent vc

Effluent Vc

4 53 4 52

17 47 13 69

25 66 22 81

4 52 4 56

17 47 14 36

26 30 27 33

9 13 8 30

OLR, organic load rate; vc, variation coefficient (%).

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reactors. Among several possible formulations for supplemental phosphorus and potassium, KH2PO4 is recommended owing to its buffer capacity.15

Although the vinasse from sugarcane contains high concentrations of potassium ion, approximately 5gL-1,20 it was used as the source of phosphorus KH2PO4 because, according to Chen et al.,29 almost no reports of toxic effects of potassium on the microbiota of anaerobic reactors.

The ratio of COD:N:P in the influent varied from 350:4.8:0.8 to 350:7.4:1.7 in R1 and R2 for OLR greater than 2.5 g total COD (Ld)-1. These values are close to that suggested by Chernicharo12 sufficient for satisfying the conditions of microorganisms for anaerobic digestion.

In the effluent of the reactors, totalP and KN were lower than those observed in the influent and ranged from 44 to 367mgL-1 and 10 to 52mgL-1, respectively. The average removal efficiencies of KN and totalP were 27-40% and 30-61%, respectively, in R1 and 30-36% and 15-49%, respectively, in R2 (Table 7). According to Oliveira et al.,30 one of the possible mechanisms for nitrogen and phosphorus removal is the formation of struvite (NH4MgPO4-6H2O) and vivianite (Fe3(PO4)2-8H2O) in addition to immobilization in the micro-biota from the sludge blanket.

The potassium concentration in the effluent of the reactors was similar to that in the influent (Table 7). Therefore, the KN, totalP, and K available in the effluent and retained in the sludge can be used for fertigation and fertilization, and thus can partly replace mineral fertilizers and reduce production costs.

Conclusions

The highest totalCOD conversion into methane of 0.19 L CH4 (gtotalCOD removed)-1 was achieved after 140 days of operation of the UASB reactors with totalCOD removal efficiencies of approximately 70% and 80% and an OLR of 5.0-7.5 gtotalCOD (L d)-1. The highest totalCOD removal efficiencies were 81% and 82% in R1 and R2, respectively, with an OLR of 2.5-5.0 gtotalCOD (Ld)-1. Recirculation of the effluent allowed adjustment of influent pH without the need to add sodium hydroxide. The UASB reactors produced methane with high efficiency, a better quality effluent, and stable sludge. The nutrients present in the vinasse and those obtained from the supplements in the anaerobic treatment can be recycled by using the effluent in fertigation and the sludge for plant fertilization.

Conflicts of interest

The authors declare no conflicts of interest.

Acknowledgements

We would like to thank CAPES and CNPQ for the financial support for this work.

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