Scholarly article on topic 'Models of endometriosis and their utility in studying progression to ovarian clear cell carcinoma'

Models of endometriosis and their utility in studying progression to ovarian clear cell carcinoma Academic research paper on "Biological sciences"

Share paper
Academic journal
J. Pathol.
OECD Field of science

Academic research paper on topic "Models of endometriosis and their utility in studying progression to ovarian clear cell carcinoma"

Journal of Pathology

J Pathol 2016; 238: 185-196 Published online in Wiley Online Library ( DOI: 10.1002/path.4657


Models of endometriosis and their utility in studying progression to ovarian clear cell carcinoma

Claire M King,1 f Cynthia Barbara,11" Andrew Prentice,1 James D Brenton2'3 and D Stephen Chamock-Jones1'3*

1 Department of Obstetrics and Gynaecology, University of Cambridge, The Rosie Hospital, Cambridge, UK

2 Functional Genomics of Ovarian Cancer Laboratory, CR-UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge, UK

3 National Institute for Health Research, Cambridge Comprehensive Biomedical Research Centre, Cambridge, UK

Correspondence to: DS Charnock-Jones, Department of Obstetrics and Gynaecology, University of Cambridge, The Rosie Hospital, Robinson Way, Cambridge CB2 0SW UK. E-mail:

^These authors contributed equally to this article.


Endometriosis is a common benign gynaecological condition affecting at least 10% of women of childbearing age and is characterized by pain - frequently debilitating. Although the exact prevalence is unknown, the economic burden is substantial (~$50 billion a year in the USA alone) and it is associated with considerable morbidity. The development of endometriosis is inextricably linked to the process of menstruation and thus the models that best recapitulate the human disease are in menstruating non-human primates. However, the use of these animals is ethically challenging and very expensive. A variety of models in laboratory animals have been developed and the most recent are based on generating menstrual-like endometrial tissue that can be transferred to a recipient animal. These models are genetically manipulable and facilitate precise mechanistic studies. In addition, these models can be used to study malignant transformation in epithelial ovarian carcinoma. Epidemiological and molecular evidence indicates that endometriosis is the most plausible precursor of both clear cell and endometrioid ovarian cancer (OCCA and OEA, respectively). While this progression is rare, understanding the underlying mechanisms of transformation may offer new strategies for prevention and therapy. Our ability to pursue this is highly dependent on improved animal models but the current transgenic models, which genetically modify the ovarian surface epithelium and oviduct, are poor models of ectopic endometrial tissue. In this review we describe the various models of endometriosis and discuss how they may be applicable to developing our mechanistic understanding of OCCA and OEA.

© 20I5 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

Keywords: endometriosis; clear cell ovarian cancer; endometrioid ovarian cancer; menstruation; animal model

Received 20 August 2015; Revised 6 October 2015; Accepted 6 October 2015

No conflicts of interest were declared.


Endometriosis is defined as the presence of endometrial tissue (glands and stroma) outside the uterine cavity; it is found primarily in the pelvis and can involve all organs but particularly the ovaries and pelvic peritoneum. It is a common condition affecting an unknown number of women of reproductive age. Typical symptoms include pain and subfertility, and the complexity and severity of the pain can present major management challenges [1,2]. Dysmenorrhoea affects up to 62% of women with endometriosis, and is incapacitating in up to 28% [3].

Definitive diagnosis requires laparoscopy or laparotomy and histological confirmation, but there is often a protracted time to diagnosis (mean of 8.0 and 11.7 years in the UK and USA, respectively) [4], due to the

complexity of the presenting symptoms. The annual economic costs associated with delays in diagnosis are substantial and were estimated at approximately $50 billion in the USA [5]. Management of pain symptoms often combines analgesia, hormonal therapies such as hormonal contraceptives, progestagens (either systemic or more commonly local delivery of levonorgestrel to the uterus), or gonadotropin-releasing hormone (GnRH) analogues. Surgical treatments include ablation or excision of endometriotic lesions or ovarian cystectomy and occasionally hysterectomy and bilateral salpingo-oophorectomy. The treatment of associated subfertility is challenging and may be surgical or by the use of assisted reproduction such as IVF. However, surgery carries risks of complications which may themselves compromise fertility. Finally, adhesiolysis and treatment of lesions carries a risk of injury to

© 20I5 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

all pelvic structures [6]. These treatment modalities vary in their effectiveness in relieving the pain and increasing pregnancy rates, and also in their side-effect profiles [7].

The concentration of a range of inflammatory mediators - cytokines, growth factors, eicosanoids, and angiogenic factors - is altered in the peritoneal fluid of women with endometriosis [8-10]. Disturbance of several of the immunomodulators has been implicated in the endometriosis-associated infertility [11]. Here, we review the relationship between endometriosis and clear cell and endometrioid ovarian cancers and the experimental models that may be used to investigate the mechanisms underlying disease progression.

The origin of endometriosis

At present, there is no definitive answer for the origin of endometriosis, but the most widely accepted theory is that it arises from endometrium that has disseminated to ectopic sites, most commonly by retrograde menstruation [12-14]. Thus, menstrual tissue passes through the fallopian tube and into the peritoneum, where it attaches, invades, and grows into endometriotic lesions. There is a genetic component to endometriosis risk and a genome-wide association study has identified candidate risk alleles [15].

Endometriosis has high prevalence, significant morbidity, and considerable economic impact. The wide range of treatments used in clinical practice underscores our limited understanding of the pathogenesis of endometriosis and approaches that exploit new models and analytical techniques could have high utility. Furthermore, although endometriosis itself is a benign disease, several epidemiological studies have identified it as a risk factor for some types of malignancies, most notably ovarian cancer, and particularly ovarian clear cell adenocarcinoma (OCCA) or ovarian endometrioid adenocarcinoma (OEA). Models which allow the underlying mechanisms to be elucidated offer the prospect of patient stratification and over the longer term, new therapies.

Endometriosis and ovarian cancer

Epithelial ovarian cancers (EOCs) are not a single disease but rather a group of diseases with distinct morphological and molecular features, all of which may affect the ovary. The principal subtypes of EOCs are high-grade serous carcinoma, low-grade serous carcinoma, mucinous carcinoma, clear cell adenocarcinoma (OCCA), and endometrioid adenocarcinoma (OEA). These tumours likely have different cells of origin, distinct histopathological features, and exhibit different molecular profiles. However, all subtypes of EOCs are treated by surgical debulking and platinum-based chemotherapy. OCCA and OEA have high rates of primary resistance to platinum-based chemotherapy which contributes to their poor prognosis when presenting with late stage or reoccurrence.


Endometriosis is now recognized as a likely precursor of some subtypes of epithelial ovarian cancer. As early as 1925, Sampson made the observation that cancer developed in ectopic endometrial tissue [16]. Since then, multiple epidemiological studies have investigated the association between endometriosis and ovarian cancer. Two large studies were carried out using the Swedish Inpatient Register (NSIR) and National Swedish Cancer Registry. In the first [17], the authors studied all patients discharged from hospital with a diagnosis of endometriosis between 1969 and 1983 - a total of 20 686 patients. Among patients with well-documented endometriosis, there was an increased risk of ovarian cancer (SIR, defined as 'the ratio of the observed numbers of cancer to those expected', 1.9; 95% CI 1.3-2.8) and the risk was particularly elevated in subjects with a long-standing history of ovarian endometriosis (SIR 4.2; 95% CI 2.0-7.7). The second study also used the NSIR to identify a large cohort of women diagnosed with endometriosis between 1969 and 2000 [18]. When 25 430 women with ovarian endometriosis were analysed, the risk of ovarian cancer was increased, with an SIR of 1.77 (95% CI 1.38-2.24), and this peaked 3-4 years after hospitalization to an SIR of 2.64 (95% CI 1.2-5).

A pooled analysis of 13 case-control studies (23 144 women) was used to examine the consistency and degree of association between endometriosis and the different subtypes of ovarian cancer [19]. Self-reported endometriosis was consistently found to be associated with an increased risk of invasive epithelial ovarian cancer after taking into account confounding factors (including study site, ethnic origin, oral contraceptive pill use, and parity). Risk was strongly associated with OCCA and OEA subtype (OCCA odds ratio 3.05, 95% CI 2.43-3.84, p < 0 • 0001; OEA odds ratio 2.04, 95% CI 1.67-2.48, p < 0.0001; and low-grade serous odds ratio 2.11,95% CI 1.39-3.20,p < 0.0001). However, the history of endometriosis in these case-control studies was self-reported and this may predispose to recall bias. While it is presumed that cancer cases may be more likely to report a history of endometriosis than controls, it is likely that this bias is random and may not account for the difference in association between the different subtypes of ovarian cancer.

Molecular evidence for the relatedness of endometriosis to OCCA and OEA

The consistent finding of increased risk in the above epidemiological studies supports a two- to three-fold increase in the risk of OCCA and/or OEA in patients with endometriosis. However, it is plausible that a common characteristic, such dysregulation of cytokine production or steroid metabolism within the peritoneal environment, predisposes to both conditions. However, there are several lines of molecular evidence that suggest that OCCA and OEA arise directly from endometriotic lesions. The first published studies used

information from loss of heterozygosity (LOH) surveys in OCCA and OEA cases to test whether identical changes were also present in adjacent endometriosis. In a study of 19 OEAs, 22 OCCAs, and 23 solitary endometrial cysts, LOH at 10q23.3 (the gene locus of PTEN) occurred in 42.1% (8 of 19) OEAs, 27.3% (6 of 22) OCCAs, and 56.5% (13 of 23) solitary endometrial cysts. Importantly, in five OEAs and seven OCCAs with synchronous endometriosis, three cases in each group showed identical LOH events in both tumour and endometriosis; one case in each group had LOH in the tumour only. The remaining endometriosis cases (one OEA and three OCCAs) did not show LOH events. None of the endometriotic lesions showed LOH events that were not also present in the co-existent tumours [20]. These data suggest that loss of PTEN function by LOH or mutation is an early event in the development of endometriosis-related cancers of the ovary.

Similar findings were demonstrated using 82 microsatellite markers to detect LOH in ten OEA and six OCCA cases with synchronous endometriosis. A total of 63 LOH events were identified in the tumours, with 22 found in the matched endometriosis tissue, and in each case, the same allele was lost. LOH was not noted in control endometriosis-only cases [21].

More recently, specific mutations and copy number abnormalities have been demonstrated in tumour and co-existent endometriosis. Wiegand et al sequenced the entire exome of 18 OCCA samples and one OCCA cell line, and the ARID1A gene in an additional 210 ovarian cancers [22]. ARID1A was mutated in 46% of OCCAs and 30% of OEAs, with 17 of the samples having two somatic mutations each. No ARID1A mutations were found in high-grade serous (HGS) specimens. Two of the patients who had OCCA samples with ARID1A mutations also had contiguous atypical endometriosis (ie with epithelial cells showing nuclear enlargement, crowding, slight hyperchromasia, and possible chromocen-tres/nucleoli and/or architectural abnormalities). One of these patients had a truncating mutation and the other had two somatic mutations predicting loss of BAF250a protein (encoded by ARID1A). These mutations were found in the contiguous atypical endometriosis but not in distant endometriosis, further supporting local transformation of endometriosis tissue. Immunohistochemical staining for BAF250a was also lost in both OCCA and the contiguous atypical endometriosis but not in distant endometriosis [22]. Taken together, these data strongly support a model for early mutation of ARID1A during malignant transformation of endometriosis.

These results were confirmed using immunohisto-chemistry for BAF250a [23] in an independent series of matched OCCA and endometriosis tissues. In that study, the authors also sequenced exons 9 and 20 of PIK3CA and found synchronous mutations in 17 of 42 (40%) of these tumours, most of which (71%) were also ARID1A-deficient [23]. This suggests that PIK3CA mutations also occur at a very early stage in the development of OCCA, before the appearance of the atypical precancerous lesions.

The MET proto-oncogene is frequently overex-pressed in OCCA and is frequently amplified. Copy number alterations and MET protein levels were studied in 13 tumours and precursor lesions [including 11 endometriosis and two clear cell adenofibromas (CCAFs)]. MET copy number gain and overexpression were demonstrated in the atypical precursor lesions. Non-atypical endometriosis and benign CCAFs did not demonstrate MET gain [24]. These data suggest that MET gain is an early event in tumourigenesis in a subset of OCCAs and that it is a driver mutation.

To further identify the pattern of somatic mutations in OCCA and investigate whether there was a clonal relationship between benign gynaecological lesions, including endometriosis, and OCCA, whole-genome sequencing of seven OCCAs was performed and targeted sequencing of other co-existent adjacent or metastatic tumours, endometriosis, and other benign conditions [25]. Most of the mutations found in the index tumour were also found in the other co-existent cancer or borderline tumours in the same patient. There were clusters of mutations present in both OCCA and at least one focus of atypical or non-atypical endometriosis from the same patient. No somatic mutations were found in normal endometrium or non-endometriosis benign lesions. Thus, atypical endometriosis shared nearly all of the coding somatic mutations that were detectable in the co-existent tumour and it is highly likely that these mutations are ancestral events in the evolution of the clear cell tumour. These findings support a model in which different subtypes of endometriosis have differing malignant potential - however, the morphological diagnosis of atypical endometriosis is difficult and these data may also suggest a local field effect for mutational change.

The studies described above, considered collectively, make a compelling case for endometriosis being the precursor lesion of OCCA and OEA - although this progression is rare. The major clinical challenge is now to determine which individuals are at higher risk of the rare event of transformation of endometriosis to OCCA or OEA. However, our understanding of the molecular basis of this progression is far from complete. Effective patient screening and therapeutic intervention will only be possible once we have a fuller picture of the molecular pathways involved. Consequently, in the absence of any effective screening for any ovarian cancer type, current guidance is that no action be taken to monitor this potential progression in women with endometriosis [6]. Such investigations are not possible in human subjects, so it is necessary to use animal models. However, these models need to be carefully selected and their limitations recognized. The establishment, development, and rare transformation of endometriosis take place in a complex environment and the selected model should replicate this as closely as possible while allowing specific manipulation and appropriate analysis. The importance of the tissue microenvironment and the selective genetic modification at defined times has been well described in cancer models [26]. Such refined approaches should inform

the design of studies of endometriosis. Here, we review the animal models used in the study of endometriosis and explore their suitability for improving our understanding of endometriosis and whether they represent appropriate models to investigate the rare progression of endometriosis to ovarian cancer.

Animal models of endometriosis

For any system to be a useful model of a human disease, it needs to recapitulate the typical features of that condition but to be manipulable in a way that humans are not. Thus, there may be a naturally occurring veterinary condition that parallels the human disease; if so, this could be used to investigate the molecular mechanisms underlying the disease. If this is not the case, then a more interventional approach is needed. The simplest and the most commonly accepted pathological mechanism underlying endometriosis is the retrograde menstruation proposed by Sampson [27]. All the models of endometriosis assume this mechanism and rely on transferring endometrium to a site outside the uterine cavity.

Spontaneous endometriosis in primates

Unlike other model organisms, some primates menstruate and develop spontaneous endometriosis and this is very similar to the human condition. For example, rhesus monkeys (Macaca mulatta) develop endometriosis that "anatomically and clinically, appears to be identical to the human disease" [28]. The frequency in a captive breeding colony was ~1%, although in a more recent study of animals aged > 10 years the prevalence was ~31% [29]. In baboons (Papio anubis), the frequency is broadly similar - 11% or 32% in animals held in captivity for less than 1 year or greater than 2 years, respectively. This difference may be due to an increase in the number of menstrual cycles (due to a reduction in the number of pregnancies) [30].

Artificial induction of endometriosis in primates

Spontaneous endometriosis in primates can take several years to develop. However, to study the onset and progression of the disease, the variability and length of time required for development of endometriosis are a major limitation. This has been partially alleviated by the development of methods that artificially induce endometriosis in primates. In 1953, Scott et al diverted the normal menstrual flow by repositioning the cervix and clamping the descending uterine vessels [31]. The ten rhesus monkeys used in this study were then monitored a year later, after which five had viable endome-trial stroma and glands, smooth and striated muscle, and fibrous tissue in the area to which the diversion was made. Similar results were obtained in baboons in which the volume of retrograde menstrual flow was increased by surgical occlusion of the cervix [32].

Induction of endometriosis by transplantation

Endometriosis is characterized by endometrial tissue being 'in the wrong place'. Hence, a reasonable approach to making an experimental model is to collect endometrium and transplant to an ectopic site.

Iatrogenic endometriosis as a complication of surgery was seen to be so common that Ridley in 1968 wrote "this fact is now growing so commonplace that it is accepted and usually not reported in the literature" [33]. Thus, it is not surprising that the obvious approach of autologous transplantation in primates was used to generate an animal model of endometriosis [34]. Surgically excised fragments of endometrium were transplanted into a number of sites in the peritoneum of rhesus monkeys. Six of the seven monkeys had viable grafts up to 522 days after transplantation. Other studies have used minced or enzymatically digested endometrium in cynomolgus monkeys (Macaca fascicularis). After 3 weeks, 76% of the subjects had endometriosis [35]. When endometrial fragments were collected during the menstrual phase and introduced into the peritoneum of baboons by injection, endometriosis developed in a very similar manner to the spontaneous disease [36]. This model showed that endometriosis could be caused by material introduced using less invasive methods and was thereby similar to humans in that the fragments seed wherever they land. However, as the material was collected by curettage, it contained tissue from layers of the endometrium that would not be found in menstrual material. A similar model, but using a pipelle (endometrial suction curette) to collect tissue at the time of menstruation, was used in the identification of ERa, MMP-7, VEGF, and aromatase in endometriotic lesions [37,38].

The effects of the presence of endometriosis on the eutopic endometrium have been studied in humans [39], and similar questions have been addressed using the baboon model. Endometriosis was induced and uterine endometrium sampled in the mid-secretory phase 1, 3, 6-7, 10-12, and 15-16 months after endometriosis induction. Transcript profiling was carried out and showed that during the early stages of the disease, there was an oestrogenic dominant phenotype in the eutopic endometrium. As the condition progressed, the endometrium became progesterone-resistant. In addition, several signalling pathways, including the ERK/MAPK and PI3/AKT, were dysregulated and expression of genes such as KRAS, FOS, and NODAL was altered [40]. Using the same approach to induce endometriosis led to increased Cyr61 (an angiogenic protein) in the eutopic endometrium [41].

In many ways, primate studies are attractive because these animals are closely related to humans, anatomically and physiologically, and spontaneously develop the disease, suggesting that the peritoneal environment is similar. These studies exemplify the usefulness of the baboon as a model animal for the study of endometriosis - the endocrine environment is similar to that in humans and can be well controlled; the induction of endometriosis is uniform and under tight

temporal control; it is possible to visualize the lesions and collect samples over a prolonged time; and, lastly, the animal cycles naturally. This is counterbalanced by more complex handling and housing requirements; thus, experimental work with primates is costly and ethically challenging.

Rodent and rabbit models

Laboratory animals do not menstruate and never develop spontaneous endometriosis. However, hamsters, rabbits, and particularly rats and mice are widely used as scientific models for medical research, due to their short generation times and their ease of physiological and of particularly genetic manipulation. Rabbit and rodent models of endometriosis have been established based on transplanting endometrium or uterine fragments, either from the same species (homologous models) or from humans (heterologous models), to ectopic sites.

A rabbit model of endometriosis was generated to study the effects of endometriosis on fertility [42]. Endometrium was transplanted from one horn of the uterus into the peritoneum. The concentration of prostaglandin in the peritoneal fluid increased and the pregnancy rate dropped 25%. Transplanted material was still present 12 weeks later, and had become cystic and similar to the lesions seen in humans [43]. Such studies suggested that the presence of endometrium in the peritoneum caused changes in the local environment, which could explain the reduced fertility. This rabbit model was useful for identifying changes in the peritoneal environment and has the advantage that the ectopic tissue grows without supplementary oestrogen and the host ovaries and immune system are intact.

Homologous rodent models

Both rat and mouse models have been generated by autotransplantation of fragments of the uterus to the mesentery [44,45]. This tissue developed into fluid-filled cysts which grew for approximately 2 months, before remaining stable for at least 10 months (in rats). In both species, epithelial and stromal cells were present. However, in this model, both the endometrium and the myometrium were transplanted, and so the resulting cysts were made up of both endometrial and myometrial tissue. Given that endometriosis by its very nature is endometrium-derived, this model does not represent an ideal model of the disease. When the endometrium was scraped from the myometrium and 'flushed' into the peritoneal cavity, no lesions developed [44].

A model in which only the endometrium is transplanted has been developed. The endometrium was collected from ovariectomized and oestrogen-treated mice. It was minced and injected into the peritoneum of syngeneic recipient animals [46]. The recipients were also treated with exogenous oestradiol and the resulting lesions were allowed to develop for ~3 weeks. Endometriosis is an oestrogen-dependent disease; indeed, as already described, many of the therapeutic strategies rely on reducing circulating oestradiol

or opposing its action. This model uses high doses of oestradiol to stimulate endometrial growth before collection and then similar doses to stimulate lesion growth in the recipient animals. The authors recognize that this may be one of the reasons why they were able to generate lesions when previous attempts without oestrogen stimulation failed. This strong oestrogen drive may limit the usefulness of this model and women with endometriosis do not have elevated oestradiol levels.

An enhancement of this model which improved the quantitative assessment of lesion growth made use of donor mice in which green fluorescent protein (GFP) was ubiquitously expressed [47]. As the donor cells are all genetically tagged and can be readily detected by fluorescent microscopy, the location and the intermixing of donor and recipient cells can be observed. These authors also demonstrated that the lesions grew poorly in the absence of exogenous oestradiol [47].

While it is possible to image GFP in the internal organs of a mouse [48] (and hence endometriotic lesions), the sensitivity is relatively low. Bioluminescent imaging using luciferase-expressing cells has greater sensitivity and allows non-invasive monitoring of lesion development. Transgenic mice with the human ubiqui-tin C promoter coupled to firefly luciferase have been used as the donors in the endometriosis model described above [45,49]. The uterus of a UbC-Luc+'+ mouse was collected and the horns were opened to allow numerous 2-mm tissue samples to be punched out. Four of these were then sutured into separate peritoneal locations in albino recipient mice. The luciferase-derived signal level was measured 2 weeks post-surgery and showed a clear signal in the location of suture sites. The mice were culled 2 weeks later and the lesions examined. All showed similarity to human endometriosis lesions, with evidence of angiogenesis. This approach is a significant advance as lesion growth can be serially monitored non-invasively for up to 35 days [49]. The origin of infiltrating cells can be tracked in this model using genetically tagged donor (with GFP, for example) or recipient animals. This model is well suited to monitor lesion response to drugs that inhibit new vessel formation [50]. Nonetheless, it has the limitations of the transplantation models described above, due to the presence of both the endometrium and the myometrium.

Spontaneous mouse endometriosis

In 2005, the first mouse model in which endometriosis developed without any form of transplant was described [51]. Activating K-ras in loxP-Stop-loxP-K-rasG12D/+ mice (LSL-K-rasG12D/+) by injecting Cre-adenovirus into the ovarian bursa resulted in the development of endometriotic-like lesions (endometrial glands but no stroma) in the ovarian surface epithelium in all the mice, and endometriotic lesions with both glands and stroma in the peritoneum in 47% of the animals. When similarly transduced ovaries were transplanted, the endometriotic-like lesions were limited to the

ovarian surface epithelium. This suggests that the endometriotic-like lesions on the ovary in this mouse model most likely arose from the ovarian surface epithelium itself. However, the peritoneal lesions probably arose from uterotubal cells in which K-ras was activated along the needle track. Injection of AdCre virus directly into the peritoneal cavity of the LSL-K-rasG12D/+ mice did not lead to the development of any endometriotic-like lesions, suggesting (in mice at least) that endometriosis does not arise through meta-plastic transformation of the peritoneum [51]. These authors exploited their novel methodology and introduced additional mutations. Injection of AdCre virus into the ovarian bursa of LSL-K-rasG12D/+;PtenloxP/loxP mice leads to signs of invasive ovarian endometrioid adenocarcinoma between 7 and 12 weeks later. However, the unintended transduction of tubular cells (which was clearly demonstrated in this study) may in fact be an important characteristic of this method of delivering the Cre. While the method provides clear information about the function of the genes manipulated, conclusions about the precise cell of origin need to be drawn cautiously. Nonetheless, the presence of the K-ras mutation in this model overcame the need for additional hormone stimulation and provides a useful model to study the histomorphology and biological behaviour of endometriosis.

'Menstrual' rodent models

Endometriosis is a condition in which normal menstrual material persists in an abnormal environment. Thus, an animal model of this condition would ideally mimic the processes that allow this material to adhere within the peritoneum and develop into a lesion. Indeed, this was the basis of the initial models in primates. Clearly, rodent models have many attractions but they do not menstruate naturally.

A mouse model has been developed to mimic the endometrial breakdown seen in human menstruation. In this model, ovariectomized mice are treated with oestra-diol and progesterone and the endometrium is stimulated to decidualize by injecting a small amount of oil directly into the uterine lumen. Examination 48 h after cessation of the hormone injections (ie progesterone withdrawal, as occurs at the end of the menstrual cycle) revealed blood vessels filled with swollen erythrocytes, apoptotic changes, and invasion of leukocytes. By 79 h post-withdrawal, blood cells and degenerating decidual cells had been shed into the uterine lumen [52]. This model is suitable for exploring the processes that take place in humans following luteal regression; however, one of the limitations of this model is the variation in the endometrial response.

This model has been refined: C56J/Bl6 ovariec-tomized mice were exposed to a programme of oestrogen injections to mimic the levels seen in the human cycle. However, progesterone was delivered via an implant inserted prior to the injection of oil into the uterus. The removal of the implant 49 h after the

decidualizing stimulus allowed a more dramatic drop in serum progesterone levels, more closely representing the human cycle. Mice were sacrificed and the uterine response was examined 16, 24, 36, and 48 h post-progesterone withdrawal. After 16 h, breakdown of the endometrium was observed; by 24 h, the decidual zone had separated from the rest of the endometrium. Tissue debris was fully cleared by 36 h after progesterone withdrawal and by 48 h, the endometrium was restored to its pre-decidual state [53]. A similar study with additional molecular analysis has also been carried out [54].

These two studies form the basis of related but distinct models of endometriosis [55,56]. In both cases, mouse endometrium is decidualized by hormone pre-treatment and an interuterine injection of oil. Progesterone treatment is withdrawn and 2.5-4 days later, 'menstrual' endometrium is collected and transferred to a recipient animal. The two models differ in the duration and strength of the progesterone-stimulated decid-ual growth: Cheng et al do not give progesterone after the injection of the oil into the uterine lumen [55]. In contrast, Greaves et al leave a progesterone implant in place for 4 days after the interuterine oil injection [56].

The work of Dinulescu et al (described above) showed that activation of K-ras was sufficient to generate an endometriosis-like lesion in the peritoneum of mice [51]. These authors suggested the possibility of "the peritoneal endometriosis having a uterine or tubal origin" in the LSL-K-rasG12D/+ mice. Thus, a small number of cells in which the LSL-K-rasG12D/+ had been activated were sufficient to lead to endometriosis.

Cheng etal used a LSL-K-rasG12V/+/Ah-Cre+/+/ ROSA26R-LacZ +/+ mouse in which Cre was induced in the uterus by P-naphthoflavone dissolved in the oil used as the decidualizing stimulus [55]. The Cre then locally activated the mutated (active) K-ras and the LacZ reporter. After progesterone withdrawal, this endometrium was transferred to a subcutaneous pocket in wild-type intact animals. No exogenous steroids were administered to the recipient animals but lesion growth could be inhibited by treatment with an oestrogen receptor antagonist (fulvestrant, ICI182780). In the absence of the genetic modification, the 'menstrual' endometrium did not form lesions. Somewhat surprisingly, only a small proportion of the cells in the endometriotic lesions were LacZ-positive, suggesting that Cre-mediated recombination did not occur in all the endometrial cells, but that this low level of K-ras activation was sufficient for lesion establishment [55]. These authors did not describe the fate of the engineered menstrual tissue if it was injected into the peritoneal cavity. Nonetheless, lesion growth was robust and accompanied by the growth of surrounding blood vessels that supplied the lesion, as illustrated in Figure 1A.

The extended progesterone treatment during decidual-ization used by Greaves et al is more efficient and yields a greater mass of 'menstrual' material for transplant. However, a significant difference in this model is that the recipient animal is primed with and continues to receive

Figure 1. Examples of lesions generated using the models described as 'menstrual' rodent models and heterologous rodent models in the main text. (A) Subcutaneous endometriotic lesion in a C57Bl/6 Tyr-'- mouse following transplantation from LSL-K-rasGuy/+/Ah-Cre+'+/ROSA26R-LacZ+'+ mice following the method of Cheng etal [55]. (B) Subcutaneous endometriotic lesion in a xenograft model in a Balb/c nu/nu mouse. Scale bar = 2 mm. The work to produce this figure was carried out in accordance with the UK Home Office Animals (Scientific Procedures) Act 1986.

oestradiol; thus there is a strong oestrogenic drive which promotes endometriotic lesion growth [56]. Despite this limitation, this model has the advantage that it is simpler than that of Cheng et al as there is no need for complex animal breeding. This is a considerable advantage as it facilitates the inclusion of other genes of interest (reporter or functional). Indeed, this approach has been effectively used to show that both host- and shed tissue-derived macrophages are present in the growing lesions [56].

Heterologous rodent models

An alternative to auto- or syngeneic-transplant is to use human endometrium and immunodeficient mice as recipients. Collectively, these have the advantage that human tissue is used, so drugs or antibodies with

species-specific effects can be used. However, they have the disadvantage that the host immune system is severely compromised. Given that endometriosis is recognized as an inflammatory condition, this is a significant weakness in these models [1].

Multiple studies have described the transplantation of proliferative, secretory, and menstrual phase human endometrium into athymic nude mice [57-62]. Tissue has been placed in or injected into the peritoneal cavity [59,60,62], or transplanted subcutaneously [58]. In some of the studies, the mice were ovariectomized and treated (or not) with oestradiol [57], and in others, the ovaries remained intact [60]. Given what is known about human endometriosis, it is not surprising that in animals treated with exogenous oestradiol, the lesions had a higher proportion of proliferating cells [61].

Studies using this model have shown that angiogene-sis is required for lesion growth, murine endothelial cells grow into the lesion, and that it is VEGF-A derived from the human tissue that drives this [60,63]; indeed, numerous blood vessels can been seen around the developing lesion shown in Figure 1B. Another useful characteristic of this model is that host and donor cells can be distinguished. An additional advantage to that obtained by using fluorescently labelled donor cells (as described earlier [47]) is that specific transcripts derived from the host or donor can also be identified [64].

These studies broadly agree on the importance of oestradiol, angiogenesis, and invasion for lesion growth. However, the models are not well suited to investigating the underlying mechanisms. For example, there is considerable heterogeneity in the tissue source - human endometrium or endometriotic lesions [65], and a variety of additional different immun-odeficient mouse strains have been used (NOD-SCID [61] and RAG-2/y(c)KO [65]). Uniform genetic modification of the human tissue fragments is challenging and while the mice can be modified by breeding in the alle-les of interest, immunodeficient mice tend not to breed very well. In an interesting approach to tackle some of these limitations, Masuda et al prepared a single-cell suspension of the glandular and stromal portions of human endometrium and transplanted this under the kidney capsule of ovariectomized NOD/SCID/ycnun mice. The mice were treated with oestrogen pellets with or without cyclic injections of progesterone. All mice transplanted were found to have endometrium-like tissue in the kidneys when collected 10 weeks later, compared with only one of the 12 non-hormone-treated mice. Mice that received oestrogen and progesterone produced larger cysts than those treated with oestrogen alone. Introduction of a YFP or luciferase gene into the human cells prior to transplantation allowed lesion growth to be followed for 8 weeks using non-invasive imaging. A cyclic increase and decrease in signal could be detected in the mice when progesterone was given or withdrawn, showing that the cyclic events of the menstrual cycle could be recapitulated in this model [66]. Such a model would also be useful in testing potential treatments of endometriosis. However, this model is

Table 1. Summary of the main characteristics of the animal models of endometriosis

Model [ref] Tissue transplanted Menstrual tissue used Immunocompetent Exogenous oestrogen required Genetic modification needed Genetically manipulable

Baboon [36] Endometrium Yes Yes No No No*

Rabbit [42] Endometrium No Yes No No No*

Rat [44] Myometrium and endometrium No Yes Yes No Yes

Mouse [45] Myometrium and endometrium No Yes Yes No Yes

Mouse [46] Endometrium No Yes Yes No Yes

Mouse [51] NA No Yes No Yes Yes

Mouse [55] Endometrium Yes Yes No Yes Yes

Mouse [56] Endometrium Yes Yes Yes No Yes

Mouse [57] Human endometrium Yes/not No Yes/no* Yes Yes§

*The donor is not currently genetically manipulable and although in principle ex vivo viral transduction would be possible, this has not been demonstrated. ♦Human endometrium from any phase of the cycle forms lesions in this model. ♦Exogenous oestradiol is frequently used but is not absolutely required.

§The donor is not genetically manipulable, although in principle ex vivo viral transduction is possible and this has been presented in abstract form (O144) [74].

limited as the recipient is immunocompromised and the site of transplantation is not typical of endometriosis, and so does not aid our understanding of adhesion to the peritoneal surface or the seeding of the ovary.

Overview of endometriosis models

As summarized above, recent animal models of endometriosis have become more refined and now are able to recapitulate the presumed pathogenesis of the human condition. As might be expected, primate models are the closest to human disease but are ethically difficult and very expensive. Rodent models, which mimic the deposition of menstrual tissue, are now available and have the advantage of being easily genetically manipulable and immunocompetent. However, the need for exogenous oestradiol in rodents may be a limitation - for example, if studying a process that is directly oestrogen-dependent. However, the complex breeding required to use the model described by Cheng et al is a significant disadvantage [55]. The principal features of the available models are summarized in Table 1.

Mouse models of epithelial ovarian cancer

It is now understood that ovarian cancer is a heterogeneous disease originating from different epithelial cells in the Mullerian tract, which show marked tropism for spread and growth on the ovary. As early as 1925, Sampson recognized this and said of ovarian endometriotic lesions: "The ovarian implants are usually more active than the latter (peritoneal), thus suggesting that ovarian tissue is the most fertile soil" [16]. Thus, any ovarian cancer model used to study the development of the disease should take into account the tissue or cell of origin and the environmental cues that may determine growth at ectopic sites. Recent elegant studies have begun to shed light on the mechanisms which may explain why the ovary is the favoured site for tumour deposition and invasion [67]. Labelled ovarian cancer-initiating cells (OTICs) were injected into the uterine horns of

superovulated mice. As early as 5 days later, fluorescent signal could be detected around the ovary by in vivo imaging. Histological examination showed labelled cells close to site of the corpus luteum and disrupted epithelium. After 10 days, the cancer cells had invaded the ovarian stroma, leaving an intact ovarian epithelium. While this experiment used OTICs derived from high-grade serous carcinoma, it demonstrates that these cells are attracted to the ovary. This is likely to be the case for the other types of epithelial ovarian cancers whose cells of origin are different but which lodge in the 'fertile soil' of the ovary.

The work of Dinulescu et al described above was a significant advance in the development of mouse models of ovarian disease because of the innovative targeting of genetic modifications to a specific site [51]. The method has been used to modify other genes implicated in ovarian cancer. For example, Arid1a loss and Pik3ca activation after intrabursal injection of AdCre in Arid1afl/fl(GT)Rosa26Pik3ca*H1047R mice leads to rapid development of ovarian cancer with OCCA-like histology [68]. Approximately half of the mice had peritoneal metastasis, often in the contralateral control (uninjected) ovary, again pointing to the propensity of tumour cells to seed in the ovary [67]. This study replicates the two molecular defects that are known to co-exist in human OCCA and thus can be used to study preventative or therapeutic options. However, a weakness of this study is the fact that OCCA is induced from OSE - or possibly uterotubual cells as found by Dinulescu et al [51]. If the tumour in fact arose in the OSE, then the model is not applicable to the study of the role of other Mul-lerian epithelia or other possible precursors, such as endometriosis in the development of OCCA. Similarly, the ablation of Arid1a and Pten (a phosphatase that inhibits the Pik3ca signalling cascade) using intrabursal AdCre in Arid1aflfl;Pten!Vfl animals led to the development of endometrioid ovarian tumours [69].

The Wnt/p-catenin pathway has been implicated in OEA by mutational profiling studies and functionally tested using models similar to those outlined above [70].

ApcloxP/loxPPtenloxP/loxP mice only develop tumours (with

the histological features characteristic of OEA) after intrabursal AdCre. The relationship of these pathways

Table 2. Summary of the main characteristics of the animal models of clear cell and endometrioid ovarian cancer

Genetic Genetically Genes

Model [ref] Immunocompetent modification needed manipulable manipulated Site of origin of tumour in mouse model

Mouse OEA model [51] Yes Yes Yes Kras, Pten OEA probably from endometriosis-like lesions

on ovarian surface epithelium (glands but

no stroma)

Mouse OCCA model [68] Yes Yes Yes Arid1a, Pik3ca OCCA arose from ovarian surface epithelium

Mouse OEA model [69] Yes Yes Yes Arid1a, Pten OEA arose from ovarian surface epithelium

Mouse OEA model [70] Yes No Yes Apc, Pten OEA arose from ovarian surface epithelium

Mouse OEA model [71] Yes No Yes Apc, Pten, Trp53 OEA arose from ovarian surface epithelium

Figure 2. Schematic diagram showing features of endometriosis and postulated mechanisms contributing to the progression of clear cell ovarian cancer. The peritoneal environment in patients with endometriosis is characterized by inflammation [56,75,76]. Exome sequencing detected somatic mutations in paired eutopic and ectopic endometrium, with a higher burden in ectopic tissue. However, "these mutations occurred in a mutually exclusive manner" [77]. Endometriotic lesions and particularly those adjacent to the OCCA carry multiple tumour-associated somatic mutations [25]. The ovary and particularly the ovulation sites are favoured seeding sites for endometriosis and probably tumour cells [67].

to other pathways implicated in ovarian cancer can elegantly be studied using enhancements of this model: for example, the role of Tp53 (which is infrequently mutated in OCAA but nearly ubiquitous in HGS) [71].

These mouse models replicate many but not all of the features of human OEAs or OCCA, but can now be extended to study in more detail the genetic alterations required for tumour development and progression. This will improve the use of these models to develop new therapies and to study treatment response. However, other models are needed to study the progression of endometriosis into OEA. If in the model the cancer arises in the OSE (the predominant target of intrabursal delivered AdCre), then this is probably the wrong cell type. On the other hand, as the mode of delivery of the Cre (intrabursal injection) also targets a modest number

of uterotubal cells, the possibility remains that these cells make a contribution to the pathology observed. The principal features of these models are summarised in Table 2.


When designing and using animal models of human disease, and specifically endometriosis, it is important to replicate the disease as closely as possible by taking into account the presumed tissue or cell of origin. The models of endometriosis all presume that the underlying mechanism depends on dissemination of endometrium by retrograde menstruation [27], and such models have been developed in non-human primates and

Identification of mutations in human disease

'Menstrual mouse' model of endometriosis (see main text)

Modify mouse endometrium, by breeding, with or without temporal induction or ex vivo by lentiviral

Transplant into wild-type or genetically modified recipient mouse

Mechanism of possible progression to clear cell carcinoma or chemo-resistance

Figure 3. Schematic diagram of how the recently developed models of endometriosis in mice can be applied to the study of progression to clear cell carcinoma.

more recently in mice. These allow precise cellular and molecular studies of the pathogenesis of endometriosis but challenges remain. In particular, pain is a critical symptom of endometriosis and the ideal model should enable investigation of the complex interaction between the endometriotic tissue, the inflammatory peritoneal environment, locally growing nerve fibres, and the central nervous system. Some of these interactions are now being investigated [72,73].

The epidemiological and the molecular data reviewed above very strongly suggest that the genesis of OCCA and OEA is not in the ovary - although the ovary is clearly a favoured seeding site. Thus, when developing improved models to study cancer progression, new approaches for targeting endometriosis cells in an appropriate environmental context are now required. The current models very elegantly demonstrate the interplay between genes and do cast light on the processes important for uncontrolled growth in the peritoneal environment. However, they do not fully address the nature of the presumed progression in humans. The nature of somatic changes necessary for the progression from eutopic endometrium to endometriosis, and then (rarely) to ovarian clear cell or endometrioid carcinoma, remains to be defined. As indicated in Figure 2, the peritoneal environment is likely to be a key determinant in this process.

Over the last 5 years, animal models of endometrio-sis and those developed to study epithelial ovarian cancer have improved significantly. There is now the prospect of combining genetic modification of

murine endometrium with the 'menstrual mouse' models of endometriosis to define the role of single or combinations of particular genes in endometriosis and OCCA or OEA. Potential risk alleles identified from human genetic studies can be functionally tested in a model of the presumed precursor lesion. An outline of this approach is shown in Figure 3. Such advances in both fields could lead to new diagnostic and therapeutic strategies and this opportunity should be exploited for the benefit of women.


CMK was funded by a grant from Cancer Research UK (CRUK; A13095). Part of the research work disclosed in this article is funded by the Strategic Educational Pathways Scholarship (Malta) to CB. The scholarship is part-financed by the European Union - European Social Fund (ESF) under Operational Programme II - Cohesion Policy 2007-2013, 'Empowering People for More Jobs and a Better Quality of Life'. JDB is supported by CRUK (A15601).

Author contribution statement

All authors contributed to the writing of the manuscript and approved the final version.


1. Laux-Biehlmann A, D'Hooghe T, Zollner TM. Menstruation pulls the trigger for inflammation and pain in endometriosis. Trends Pharmacol Sci 2015; 36: 270-276.

2. McKinnon BD, Bertschi D, Bersinger NA, et al. Inflammation and nerve fiber interaction in endometriotic pain. Trends Endocrinol Metab 2015; 26: 1-10.

3. Bellelis P, Dias JA Jr, Podgaec S, et al. Epidemiological and clinical aspects of pelvic endometriosis - a case series. Rev Assoc Med Bras 2010; 56: 467-471.

4. Hadfield R, Mardon H, Barlow D, et al. Delay in the diagnosis of endometriosis: a survey of women from the USA and the UK. Hum Reprod 1996; 11: 878-880.

5. Simoens S, Dunselman G, Dirksen C, et al. The burden of endometriosis: costs and quality of life of women with endometriosis and treated in referral centres. Hum Reprod 2012; 27: 1292-1299.

6. Dunselman GAJ, Vermeulen N, Becker C, et al. ESHRE guideline: management of women with endometriosis. Hum Reprod 2014; 29: 400-412.

7. Brown J, Farquhar C. An overview of treatments for endometriosis. J Am Med Assoc 2015; 313: 296-297.

8. McLaren J, Prentice A, Charnock-Jones DS, et al. Vascular endothe-lial growth factor is produced by peritoneal fluid macrophages in endometriosis and is regulated by ovarian steroids. J Clin Invest 1996; 98: 482-489.

9. Hornung D, Ryan IP, Chao VA, et al. Immunolocalization and regulation of the chemokine RANTES in human endometrial and endometriosis tissues and cells. J Clin Endocrinol Metab 1997; 82: 1621-1628.

10. Monsivais D, Bray JD, Su E, et al. Activated glucocorticoid and eicosanoid pathways in endometriosis. Fertil Steril 2012; 98: 117-125.

11. de Ziegler D, Borghese B, Chapron C. Endometriosis and infertility: pathophysiology and management. Lancet 2010; 376: 730-738.

12. Sampson JA. Metastatic or embolic endometriosis, due to the menstrual dissemination of endometrial tissue into the venous circulation. Am J Pathol 1927; 3: 93-110.43.

13. Burney RO, Giudice LC. Pathogenesis and pathophysiology of endometriosis. Fertil Steril 2012; 98: 511-519.

14. Vercellini P, Vigano P, SomiglianaE, etal. Endometriosis: pathogenesis and treatment. Nature Rev Endocrinol 2014; 10: 261-275.

15. Nyholt DR, Low S-K, Anderson CA, et al. Genome-wide association meta-analysis identifies new endometriosis risk loci. Nature Genet 2012; 44: 1355-1359.

16. Sampson JA. Endometrial carcinoma of the ovary, arising in endometrial tissue in that organ. Arch Surg 1925; 10: 1 -72.

17. Brinton LA, Gridley G, Persson I, et al. Cancer risk after a hospital discharge diagnosis of endometriosis. Am J Obstet Gynecol 1997; 176: 572-579.

18. Melin A, Sparén P, Persson I, et al. Endometriosis and the risk of cancer with special emphasis on ovarian cancer. Hum Reprod 2006; 21: 1237-1242.

19. Pearce CL, Templeman C, Rossing MA, et al. Association between endometriosis and risk of histological subtypes of ovarian cancer: a pooled analysis of case-control studies. Lancet Oncol 2012; 13: 385-394.

20. Sato N, Tsunoda H, Nishida M, etal. Loss of heterozygosity on 10q23.3 and mutation of the tumor suppressor gene PTEN in benign endometrial cyst of the ovary: possible sequence progression from benign endometrial cyst to endometrioid carcinoma and clear cell carcinoma of the ovary. Cancer Res 2000; 60: 7052-7056.

21. Prowse AH, Manek S, Varma R, et al. Molecular genetic evidence that endometriosis is a precursor of ovarian cancer. Int J Cancer 2006; 119: 556-562.

22. Wiegand KC, Shah SP, Al-Agha OM, et al. ARID1A mutations in endometriosis-associated ovarian carcinomas. N Engl J Med 2010; 363: 1532-1543.

23. Yamamoto S, Tsuda H, Takano M, etal. Loss of ARID1A protein expression occurs as an early event in ovarian clear-cell carcinoma development and frequently coexists with PIK3CA mutations. Mod Pathol 2012; 25: 615-624.

24. Yamamoto S, Tsuda H, Miyai K, et al. Accumulative copy number increase of MET drives tumor development and histological progression in a subset of ovarian clear-cell adenocarcinomas. Mod Pathol 2012; 25: 122-130.

25. Anglesio MS, Bashashati A, Wang YK, et al. Multifocal endometri-otic lesions associated with cancer are clonal and carry a high mutation burden. J Pathol 2015; 236: 201-209.

26. Frese K, Tuveson D. Maximizing mouse cancer models. Nature Rev Cancer 2007; 7: 645-658.

27. Sampson JA. Peritoneal endometriosis due to menstrual dissemination of endometrial tissue into the peritoneal cavity. Am J Obstet Gynecol 1927; 14: 442-469.

28. MacKenzie WF, Casey HW. Animal model of human disease. Endometriosis. Animal model: endometriosis in rhesus monkeys. Am J Pathol 1975; 80: 341-344.

29. Zondervan KT, Weeks DE, Colman R, et al. Familial aggregation of endometriosis in a large pedigree of rhesus macaques. Hum Reprod 2004; 19: 448-455.

30. D'Hooghe TM, Bambra CS, De Jonge I, etal. The prevalence of spontaneous endometriosis in the baboon (Papio anubis, Papio cynocephalus) increases with the duration of captivity. Acta Obstet Gynecol Scand 1996; 75: 98-101.

31. Scott RB, Te Linde RW, Wharton LR. Further studies on experimental endometriosis. Am J Obstet Gynecol 1953; 66: 1082-1103.

32. D'Hooghe TM, Bambra CS, Suleman MA, etal. Development of a model of retrograde menstruation in baboons (Papio anubis). Fertil Steril 1994; 62: 635-638.

33. Ridley JH. The histogenesis of endometriosis: a review of facts and fancies. Obstet Gynecol Surv 1968; 23: 1-35.

34. Te Linde RW, Scott RB. Experimental endometriosis. Am J Obstet Gynecol 1950; 60: 1147-1173.

35. Sillem M, Hahn U, Coddington CC 3rd, etal. Ectopic growth of endometrium depends on its structural integrity and proteolytic activity in the cynomolgus monkey (Macaca fascicularis) model of endometriosis. Fertil Steril 1996; 66: 468-473.

36. D'Hooghe TM, Bambra CS, Raeymaekers BM, etal. Intrapelvic injection of menstrual endometrium causes endometriosis in baboons (Papio cynocephalus and Papio anubis). Am J Obstet Gynecol 1995; 173: 125-134.

37. Fazleabas AT, Brudney A, Gurates B, et al. A modified baboon model for endometriosis. AnnNY Acad Sci 2002; 955:308-317; discussion 340-342, 396-406.

38. Fazleabas AT, Brudney A, Chai D, etal. Steroid receptor and aro-matase expression in baboon endometriotic lesions. Fertil Steril 2003; 80 (Suppl 2): 820-827.

39. Kao LC, Germeyer A, Tulac S, etal. Expression profiling of endometrium from women with endometriosis reveals candidate genes for disease-based implantation failure and infertility. Endocrinology 2003; 144: 2870-2881.

40. Afshar Y, Hastings J, Roqueiro D, etal. Changes in eutopic endometrial gene expression during the progression of experimental endometriosis in the baboon, Papio anubis. Biol Reprod 2013; 88: 44.

41. Gashaw I, Hastings JM, Jackson KS, etal. Induced endometriosis in the baboon (Papio anubis) increases the expression of the proangio-genic factor CYR61 (CCN1) in eutopic and ectopic endometria. Biol Reprod 2006; 74: 1060-1066.

42. Schenken RS, Asch RH. Surgical induction of endometriosis in the rabbit: effects on fertility and concentrations of peritoneal fluid prostaglandins. Fertil Steril 1980; 34: 581-587.

43. Dunselman GA, Willebrand D, Land JA, et al. A rabbit model of endometriosis. Gynecol Obstet Invest 1989; 27: 29-33.

44. Vernon MW, Wilson EA. Studies on the surgical induction of endometriosis in the rat. Fertil Steril 1985; 44: 684-694.

45. Cummings AM, Metcalf JL. Induction of endometriosis in mice: a new model sensitive to estrogen. Reprod Toxicol 1995; 9: 233-238.

46. Somigliana E, Vigano P, Rossi G, etal. Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis. Hum Reprod 1999; 14: 2944-2950.

47. Hirata T, Osuga Y, Yoshino O, et al. Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein. Hum Reprod 2005; 20: 2092-2096.

48. Yang M, Baranov E, Jiang P, et al. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc Natl Acad Sci U S A 2000; 97: 1206-1211.

49. Becker CM, Wright RD, Satchi-Fainaro R, et al. A novel noninvasive model of endometriosis for monitoring the efficacy of antiangiogenic therapy. Am J Pathol 2006; 168: 2074-2084.

50. Zhao Y, Gong P, Chen Y, et al. Dual suppression of estrogenic and inflammatory activities for targeting of endometriosis. Science Transl Med 2015; 7: 271ra9.

51. Dinulescu DM, Ince TA, Quade BJ, et al. Role of K-ras and Pten in the development of mouse models of endometriosis and endometrioid ovarian cancer. Nature Med 2005; 11: 63-70.

52. Finn CA, Pope M. Vascular and cellular changes in the decidualized endometrium of the ovariectomized mouse following cessation of

hormone treatment: a possible model for menstruation. J Endocrinol 1984; 100: 295-300.

53. Brasted M, White CA, Kennedy TG, etal. Mimicking the events of menstruation in the murine uterus. Biol Reprod 2003; 69: 1273-1280.

54. Cheng C-W, Bielby H, Licence D, et al. Quantitative cellular and molecular analysis of the effect of progesterone withdrawal in a murine model of decidualization. Biol Reprod 2007; 76: 871-883.

55. Cheng C-W, Licence D, Cook E, et al. Activation of mutated K-ras in donor endometrial epithelium and stroma promotes lesion growth in an intact immunocompetent murine model of endometriosis. J Pathol 2011; 224: 261-269.

56. Greaves E, Cousins FL, Murray A, et al. A novel mouse model of endometriosis mimics human phenotype and reveals insights into the inflammatory contribution of shed endometrium. Am J Pathol 2014; 184: 1930-1939.

57. Zamah NM, Dodson MG, Stephens LC, etal. Transplantation of normal and ectopic human endometrial tissue into athymic nude mice. Am J Obstet Gynecol 1984; 149: 591-597.

58. Bergqvist A, Jeppsson S, Kullander S, etal. Human endometrium transplanted into nude mice. Histologic effects of various steroid hormones. Am J Pathol 1985; 119: 336-344.

59. Bruner KL, Matrisian LM, Rodgers WH, etal. Suppression of matrix metalloproteinases inhibits establishment of ectopic lesions by human endometrium in nude mice. J Clin Invest 1997; 99: 2851 -2857.

60. Nisolle M, Casanas-Roux F, Donnez J. Early-stage endometriosis: adhesion and growth of human menstrual endometrium in nude mice. Fertil Steril 2000; 74: 306-312.

61. Grummer R, Schwarzer F, Bainczyk K, et al. Peritoneal endometriosis: validation of an in vivo model. Hum Reprod 2001; 16: 1736-1743.

62. Eggermont J, Donnez J, Casanas-Roux F, et al. Time course ofpelvic endometriotic lesion revascularization in a nude mouse model. Fertil Steril 2005; 84: 492-499.

63. Hull ML, Charnock-Jones DS, Chan CLK, et al. Antiangiogenic agents are effective inhibitors of endometriosis. J Clin Endocrinol Metab 2003; 88: 2889-2899.

64. Hull ML, Escareno CR, Godsland JM, et al. Endometrial-peritoneal interactions during endometriotic lesion establishment. Am J Pathol 2008; 173: 700-715.

65. Greenberg LH, Slayden OD. Human endometriotic xenografts in immunodeficient RAG-2/y(c)KO mice. Am J Obstet Gynecol 2004; 190: 1788-1795; discussion 1795-1796.

66. Masuda H, Maruyama T, Hiratsu E, et al. Noninvasive and real-time assessment of reconstructed functional human endometrium in NOD/SCID/ycnuU immunodeficient mice. Proc Natl Acad Sci USA 2007; 104: 1925-1930.

67. Yang-Hartwich Y, Gurrea-Soteras M, Sumi N, et al. Ovulation and extra-ovarian origin of ovarian cancer. Sci Rep 2014; 4: 6116.

68. Chandler RL, Damrauer JS, Raab JR, etal. Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumori-genesis through pro-tumorigenic inflammatory cytokine signalling. Nature Commun 2015; 6: 6118.

69. Guan B, Rahmanto YS, Wu RC, etal. Roles of deletion of Arid1a, a tumor suppressor, in mouse ovarian tumorigenesis. J Natl Cancer Inst 2014; 106: dju146.

70. Wu R, Hendrix-Lucas N, Kuick R, et al. Mouse model of human ovarian endometrioid adenocarcinoma based on somatic defects in the Wnt/beta-catenin and PI3K/Pten signaling pathways. Cancer Cell 2007; 11: 321-333.

71. Wu R, Baker SJ, Hu TC, etal. Type I to type II ovarian carcinoma progression: mutant Trp53 or Pik3ca confers a more aggressive tumor phenotype in a mouse model of ovarian cancer. Am J Pathol 2013; 182: 1391-1399.

72. Greaves E, Temp J, Esnal-Zufiurre A, et al. Estradiol is a critical mediator of macrophage-nerve cross talk in peritoneal endometrio-sis. Am J Pathol 2015; 185: 2286-2297.

73. Morotti M, Vincent K, Brawn J, et al. Peripheral changes in endometriosis-associated pain. Hum Reprod Update 2014; 20: 717-736.

74. Barbara C, Dawson C, Charnock-Jones DS. Abstracts of the 62nd Annual Scientific Meeting, March 26-28, 2015, San Francisco, California [No authors listed]. Reprod Sci 2015; 22 (Suppl): 55A-390A.

75. Lin Y-J, Lai M-D, Lei H-Y, et al. Neutrophils and macrophages promote angiogenesis in the early stage of endometriosis in a mouse model. Endocrinology 2006; 147: 1278-1286.

76. Bacci M, Capobianco A, Monno A, et al. Macrophages are alternatively activated in patients with endometriosis and required for growth and vascularization of lesions in a mouse model of disease. Am J Pathol 2009; 175: 547-556.

77. Li X, Zhang Y, Zhao L, etal. Whole-exome sequencing of endometriosis identifies frequent alterations in genes involved in cell adhesion and chromatin-remodeling complexes. Hum Mol Genet 2014; 23: 6008-6021.