Scholarly article on topic 'The distribution of mercury and other trace elements in the bones of two human individuals from medieval Denmark -- the chemical life history hypothesis'

The distribution of mercury and other trace elements in the bones of two human individuals from medieval Denmark -- the chemical life history hypothesis Academic research paper on "History and archaeology"

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Academic research paper on topic "The distribution of mercury and other trace elements in the bones of two human individuals from medieval Denmark -- the chemical life history hypothesis"


The distribution of mercury and other trace elements in the bones of two human individuals from medieval Denmark - the chemical life history hypothesis

Kaare Lund Rasmussen1*, Lilian Skytte1, Christian Pileter1, Anne Lauritsen1, Jesper Lier Boldsen2, Peter Mygind Leth2 and Per Orla Thomsen3


Excavating human skeletons is the closest archaeologists can get to the people who lived in the past. Once excavated the bones are often analysed chemically in order to yield as much information as possible. Most archaeometric analyses performed on samples of human skeletal remains have been performed on a single sample from a tooth or a long bone. In this paper we investigate how a suite of elements (Mg, Al, Ca, Mn, Fe, Zn, As, Sr, Ba, Hg and Pb) are distributed in two medieval skeletons excavated at the laymen cemetery at the Franciscan Friary in Svendborg, Denmark.

The analyses have been performed using CV-AAS for Hg and ICP-MS for the rest of the elements. We find that in general Hg concentrations are highest in the trabecular tissues and in the abdomen region. Our data also show that the elements Al, Fe and Mn concentrate in the trabecular tissue and on the surfaces of the bones. The two individuals can be clearly distinguished by Principal Component Analysis of all the measured trace elements. Our data support a previously published hypothesis that the elemental ratios Sr/Ca, Ba/Ca and Mg/Ca are indicative of provenance. Aluminium, Fe and Mn can be attributed to various forms of diagenesis, while Hg is not present in sufficiently large amounts in the surrounding soil to allow diagenesis to explain the high Hg values in the trabecular tissue. Instead we propose that Hg must originate from decomposed soft tissue.

Keywords: Human bones, Medieval, Chemical life history, Mercury, Trace elements


Indicators of diet, provenance, occupation and medical treatment of medieval humans are increasingly being investigated by trace element analyses in the skeletal remains procured from archaeological excavations. These analyses are normally performed on a single sample from the skeleton of an individual, often a tooth or the compact part of the largest bone in the human body - the femur. However, little attention has been given to the distribution of the trace elements between different bone elements in the same skeleton. Grupe [1] discriminated between

* Correspondence:

institute of Physics, Chemistry and Pharmacy, University of Southern

Denmark, Campusvej55, Odense M DK-5230, Denmark

Full list of author information is available at the end of the article

compact and trabecular bone tissues and reported average trace element concentrations of Mg, P, Ca, Zn, Sr and Ba in the two types of tissue. Concentrations in different tissue types in modern man were reported in the comprehensive report Reference Man [2]. In this work we present trace element analyses of 36 different parts of the skeletons of two medieval human individuals excavated in Denmark. The two individuals were selected based on a larger survey by Rasmussen et al. [3] reporting the Hg concentrations in single samples of compact bone tissue in each individual, mostly from the femur, and including more than hundred individuals. The first individual selected for this study exhibited an elevated Hg concentration in the analysis of a single sample from the femur, indicating that this individual had been exposed to Hg, probably through medical treatment with Hg. The other

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individual was selected because the single femoral sample featured an Hg concentration at the background level, which indicated that the individual was not exposed to Hg above the environmental background level.

The samples were subjected to two analytical methods: CV-AAS for Hg and ICP-MS for Mg, Al, Ca, Mn, Fe, Zn, As, Sr, Ba and Pb. Our results show significant differences between the trace element concentrations in the various bone tissues, most notably between compact and trabecular tissue. It is the first time that a study of the bone trace element chemistry has been conducted to this level of detail on Danish medieval skeletons.

Excavation site and bone material

The Franciscan Friary in Svendborg was founded in 1236 (Figure 1). Svendborg is situated on the south coast of the island of Fyn in Denmark (Additional file 1). The buildings of the friary were modified and enlarged over the centuries and in 1361 a new gothic church was consecrated by Bishop Niels Jonsen from Odense. A layman cemetery was situated to the south of the friary's church [4]. To the east, the premises of the friary were bordered by the coastline. To the west was an elevated ground where the medieval city was situated. Excavations in 1975-1977 and again in 2007 revealed extremely well preserved burials in the laymen cemetery; in some individuals even brain tissue was preserved. The reason for the fine state of preservation was undoubtedly the highly anoxic and groundwater saturated low-porosity clay-rich soil.

A series of individuals excavated from the cemetery in 1975-77 have previously been sampled for compact bone tissue which were analysed in triplicate for Hg by Cold Vapour Atomic Absorption Spectroscopy (CV-AAS) [3]. On this basis two individuals were selected. One individual, AG93 (KLR-6578 in [3]; the present samples are designated KLR-8601), exhibited a very low Hg concentration of 16.6 ± 1.2 ng g-1, which is typical of non-exposed individuals. The other individual, AG104 (KLR-6577 in [3]; the present samples are designated KLR-8602), had a Hg concentration of 273 ± 43 ng g-1, which is relatively high, and certainly elevated above the normal background of 10100 ng g-1, and it is therefore likely that AG104 experienced an intra vitam exposure to Hg.

AG93 was a 38-45 years old male. A lesion to the nasal region makes it probable that he was once in a fight or had an accident. AG104 was a 17-19 years old male (note the revised sexing and age estimation of this individual compared to [3]). Neither individual had distinct signs of TB, leprosy or syphilis.

The bone samples were procured by drilling. Before taking a sample of compact bone tissue any contamination residing on the bone surface was removed mechanically in all but one case from each skeleton. The trabecular samples were simply drilled out as found, as

Figure 1 The archaeological site, the Franciscan Friary in Svendborg, Demark. a: The Franciscan Friary in Svendborg just prior to demolition in 1828. Watercolour by C.F. Thorin 1828 now at the National Museum of Denmark; b: The excavation plan of the lay cemetery at Svendborg Franciscan Friary (field A) showing the excavation level3 and the position of AG93 at depth 6.11 m (violet rectangle); c: Same field, excavation level 4, the position of AG104 at depth 5.94 m is indicated by a violet rectangle; from Svendborg County Museum 1977.

no mechanical de-contamination is possible on this type of tissue. There is no question that the trabecular tissues are at all times augmented by decomposed soft tissue collapsing onto the bone lamellae, and occasionally polluted by invading soil, as will be abundantly clear in the results section of this paper. The soils of Denmark are generally a mixture of organic matter and glacial deposits, which is a mixture of mainly clay but also quartz, feldspars and other minerals of very fine grain sizes capable of penetrating small cavities and cracks.

Prior to sampling the drill and all other stainless steel utensils were rinsed in distilled water and heated in an ethanol flame in order to rinse off any dust particles and water soluble salts and evaporate any Hg possibly present as contamination. Gloves, hairnet and face mask were used during sampling. The drill used was a Dremel MultiPro® electric drill model 395 with adjustable drilling velocity, operated at low velocity and equipped with a 2 mm diameter drill. In preparation for sampling, the working table was covered with a large sheet of clean white paper. A sheet of clean aluminium foil was placed on top of the paper. The bone was placed on the Al-foil and the drill was taken into the ethanol flame and left to cool ca. 30 seconds. Then a surface area of 1 to 2 cm2 was removed by drilling in order to rid the bone of the surface. The bone was brushed gently to remove remaining dust, and the Al-foil together with the surface material was removed and discarded. After rinsing and reheating the drill and letting it cool again, the drilling of the compact bone material was carried out on top of a new clean sheet of Al-foil. The drilled sample was transferred to a pre-cleaned glass and sealed. For the tra-becular samples the procedure was the same except no decontamination of the bone surface could be performed prior to sampling.

Thirty seven samples were taken from skeleton AG93 and 36 samples from AG104. In the long bones all samples were taken from the dorsal/posterior, proximal part of the bone with the exception of the set of compact bone samples from left femur and humerus. These samples were taken with an equivalent distance along the dorsal surface of the left femur and humerus in both skeletons. Table 1 summarizes the places in the skeleton from where the samples were extracted. Each sample had a weight of 20 to 40 mg.

Analytical techniques


Trace elements were measured by ICP-MS (Inductively Coupled Plasma Mass Spectroscopy). Each sample of ca. 20 mg was dissolved in a mixture of 2 mL HNO3, 1 mL H2O2, and 0.67 mL HCl all concentrated and all of ICP-MS grade. This took place in new, sealed polystyrene containers on a shaking table for 24 hours at room

Table 1 Summary of the bone elements sampled in AG93 and AG104

AG93 AG104

Femur compact 2 2

Femur trabecular 2 2

Femur compact extra 11 11

Tibia compact 1 1

Tibia trabecular 1 1

Humerus compact 2 2

Humerus trabecular 2 2

Humerus compact extra 7 7

Ulna compact 1 1

Ilium 1 1

Sacrum 3 3

Ribs 2 2

Vertebra 1 1

Tubular bone from foot 1 0

Total number of samples 37 36

temperature (ca. 20°C). The samples were then divided and half was used for ICP-MS the other half for CV-AAS. The samples were diluted to 10 mL with Milli-Q water and filtered through 0.45 ^m PVDF Q-Max disposable filters. The solutions were then stored at +4°C until the analyses were performed.

The analyses were carried out on a Bruker ICP-MS 820, equipped with a frequency-matching RF generator and a Collision Reaction Interface (CRI), operating with helium or hydrogen. The basic parameters were as follows: radiofrequency power 1.40 kW, plasma gas flow 15.50 L min-1; auxiliary gas flow 1.65 L min-1; sheath gas flow 0.12 L min- ; nebulizer gas flow 1.00 L min- . The CRI Reaction System was activated for As and Fe because of interferences with polyatomic species produced by a combination of isotopes coming from the argon plasma, reagents and matrix. A mixture of Sc45, Y89 and Tb159 was used as internal standard added to all analyses. The following isotopes were measured without skimmer gas: Mg24, Al27, Ca44, Mn55, Zn66, Sr88, Ba137 and a combination of Pb isotopes (Pb206, Pb207 and Pb208). Fe56 was measured with hydrogen as skimmer gas. As75 was run with helium as skimmer gas. The dwell time on each peak was 30 ms. There were made 5 replicate analyses of each sample and each replicate consisted of 30 mass scans. Multi-element calibration standards were prepared in 1% HNO3 at 6 different concentrations (0, 1, 10, 20, 100 and 200 ^g L-1), but for each element only 3 standards were selected to fit the appropriate concentration range in the samples. For the main element Ca three standards of 10, 20 and 25 wt% were run. The average overall uncertainties of the

reported element concentrations were (RSD at 1a expressed in %): Mg: 4.2; Al: 2.6; Ca: 2.0; Mn: 1.8; Fe: 6.9; Zn: 2.0; As: 4.6; Sr: 1.8; Ba: 1.1; and Pb: 2.2.


Mercury was measured by cold vapour atomic absorption on a dedicated mercury analyzer, a Flow Injection Mercury System (FIMS-400) manufactured by PerkinElmer and equipped with an S10 autosampler. This system featured a better detection limit for Hg than the ICP-MS, which occasionally tended to be overloaded with Hg from the continuous daily analyses of bones, some of which were high in Hg. Two hours prior to analysis 1 mL of concentrated KMnO4 was added to 10 mL of sample in order to maintain Hg on ionic form. The sample was then diluted to 20 mL. In the reaction chamber of the FIMS-400 the Hg was released as vapour by adding NaBH4. The analyzer was operated in the continuous flow mode. The overall uncertainties, i.e. including reproducibility and dilution, was estimated to be ca. ± 10% (1a), and the LOD was ca. 1.5 ng g-1 for a human bone sample of ca. 20 mg.

An in-house human bone standard was dissolved daily together with the bone samples and included in the daily runs in both ICP-MS and CV-AAS in order to monitor any drift in the systems. However, the inherent inhomo-geneity in the in-house standard (70-100 ng g-1) was in all normal cases larger than the analytical uncertainty.


A Siemens Somatom Spirit dual slice CT-scanner was used to determine the compactness of the trabecular bone tissue in immediate proximity to the sampling sites. The CT-images were viewed on a Siemens Syngo MultiModality workstation (software syngoMMWP version VE31A). The scanning parameters were as follows: current 57 mA, tension 130 kV, slice thickness 3 mm, pitch 0.95, rotation time 1.0 s, Field of View 200 mm, and system de-convolution kernel U90 (ultrahigh resolution). The Hounsfield-values were calibrated to -1000 for air and 0 for a pure water phantom. Normal Hounsfield-values for compact bone tissue were varying very little and exhibited an average of 1800. The observed Hounsfield-value for trabecular tissue, H, can be expressed:

H = 1800k - 1000(1 - k) (1)

where k is the compactness ranging from 0 to 1. Solving this equation yields for the compactness:

k = (H + 1000)/2800 (2)

Some elements are not very mobile in the grave either because they have low solubility mainly due to the formations of sulphides or because they adsorb readily to

the surfaces of the bones after decomposition of the soft parts of the body. In individuals where the concentration of an immobile element is in equilibrium between compact bone (concentration Cc), trabecular bone (concentration Ct), and soft tissue, the relative trabecular bone concentration, RCt, is one. RCt can be estimated from the average compact bone concentration and the degree of compactness, k, in the following way:

RCt = Ct -k/Cc (3)


The results of the measurements of the elemental concentrations measurements are listed in Additional file 2: Table S1 together with Relative Standard Deviations at ± 1 a expressed in % of the concentration. The compactness-values measured by CT-scanning are also listed in Additional file 2: Table S1. In the last two lines of Additional file 2: Table S1 are listed the average concentrations of the in-house bone standard and the standard deviations on the average concentrations.

The variation of the elements has been analyzed by Principle Component Analysis. Using two components and eliminating obvious outliers, more than 99% of the variation can be explained by the first component, PC1, and ca. 1% by PC2. The factor loadings for the elements can be seen in Figure 2. Iron and Mn are responsible for most of the variation; Hg, Sr, Ba, Mg and Al each are responsible for part of the variation; whereas very little variation results from Ca, Zn, As, and Pb. The PCA-scores marked according to the two individuals are shown in Figure 3a and marked according to compact and trabecular bone tissue in Figure 3b. The two individuals as well as the tissue types can be clearly distinguished in the two plots. The interpretations of the reason for the variation of each element will be

• Mn Sr Ca, Zn, As, Pb

•Me •Al * Hg Fe ■ -

0.0 0.2 0,4 0.6 0.8 1,0

Figure 2 The factor loadings determined by PCA analysis.

Figure 3 The scores determined by the PCA analysis. a: The factor scores for each sample determined by the PCA analysis: AG104 (yellow circles); AG93 (red squares). b: The factor scores for each sample determined by the PCA analysis (same data as in Figures 3a): compact tissues (green circles); trabecular tissues (blue triangles).

discussed further below. However, in short our interpretations are the following: 1) that Hg is present as a result of a combination of Hg in background levels and higher concentrations resulting from medication; 2) that Sr, Ba and Mg substitute for Ca in the bone apatite and that their variations result from differences in the bioavail-able reservoirs of these elements at various geographical locations; 3) that Fe and Mn result from postmortem deposition by groundwater; 4) that Al originates from soil contamination; and 5) that the rest of the elements are either metabolically controlled or present only at

background levels and therefore exhibiting no large variations in the material.


There is very little reason to expect a difference in the Hg concentrations between the left and the right side of the skeleton, which was indeed not seen in the data of either of the two individuals investigated here, see Figure 4, which shows the Hg concentrations in the compact bone types. The average Hg concentrations determined for the compact bone tissue was 52 ng g-1

Figure 4 Mercury measured in compact bone tissues. AG93 is shown as red points and AG104 as yellow points. The averages are indicated with horizontal lines. The error bars represent ±2 standard deviations of the totalanalyticaluncertainty. Duplicate bone elements are right and left, repectively.

for AG93 and 127 ng g-1 for AG104 (averages calculated excluding the multiple samples taken along the femur and humerus).

Provided the bone remodelling turnover rate varied among bone positions, a sudden exposure to Hg relatively late in life (i.e. in adulthood) would result in a large variation in the Hg concentration measured in different bone locations as they undergo remodelling at different rates. In order to reveal if such variation on the turnover rate exists, a series of 7-8 samples were taken along the backside of the left femur and the left humerus (Figure 5). As can be seen from Figure 5 there is no systematic variation along either the femur or the humerus. For the non-exposed individual AG93, the concentrations are at the background with the only exception of the last sample of the femur, which included small amounts of trabecular tissue. For AG104, who had been exposed to Hg while alive, the Hg concentrations were generally higher than in AG93. In both cases our data indicate that there is no systematic variation in the Hg concentration in the compact tissue along the femur or humerus, and that the bone remodelling rate therefore can be assumed to be the constant along the femur and humerus.

In contradiction to the uniformity of the Hg in compact tissue, the trabecular tissue exhibited a much greater variation in Hg concentrations as can be seen in Figure 6. The Hg concentrations in trabecular tissue are up to 40 times higher than the in the compact tissue, an observation which will be discussed below.

Aluminium, Fe and Mn

The measured Al concentrations are shown for both individuals in Figure 7. As can be expected little or no

contamination or soil invasion was observed in the compact bone tissue. The average Al concentration the compact bone samples from AG93 was 13.2 ^g g-1, and 26.4 ^g g-1 for AG104. In the trabecular tissue the Al was ranging from 63 to 847 ^g g-1, which is a factor of up to 50 higher than in the compact tissue. There is normally low levels of Al in the human skeletal organ (<1.1 to 5.0 ^g g-1, 80% range, [2]), so most of the Al found in this study must originate from clay minerals in soil which has polluted the bones. This is consistent with the up to 50 times higher Al concentrations sometimes found in the trabecular tissues, which could not be mechanically decontaminated.

The analyses of Fe and Mn are shown in Figure 8. Both element concentrations outnumber the concentrations normally found in modern man (42-160 ^g g- for Fe and 0.32-0.98 ^g g-1 for Mn, both at the 80% range [2]), with values which are ca. 100 and 10,000 times higher, for Fe and Mn respectively. There is no doubt that both Fe and Mn, possibly under different geochem-ical conditions, are deposited onto the bones by the groundwater. Natural cavities in the bones and small postmortem cracks will allow these elements to be deposited to some degree also in compact bone tissue.

Calcium, Sr, Ba, and Mg

The elemental ratios of Sr, Ba and Mg to Ca are shown in Figure 9. It is characteristic that in AG104 all three elemental concentration ratios are more or less similar in both compact and trabecular tissues. Since there is no difference between compact and trabecular tissue in general, it is unlikely that any of these elements are brought to the bones by diagenetic processes. AG93 shows more variation and generally higher Ba/Ca and

(a) Femur

o- AG 104 —•— AG93 t

15 20 25 30 Distance along bone (Cm)

CD 100


—o- AG 104 —AG93

15 20 25 30 Distance along bone (cm)

Figure 5 Mercury measured along femur and humerus. a: Hg measured on compact bone samples taken along the posterior of femur. X-axis in cm, zero is at the top of caput femoris. b: Hg measured on compact bone samples taken along the backside of humerus. X-axis in cm, zero is at the top of caput humeri. The last sample in the femur of AG93 also included smallamounts of trabecular tissue. The error bars represent ± 2 standard deviations of the totalanalyticaluncertainty.

Figure 6 Mercury measured in compact and trabecular tissues. AG104 marked with yellow points, AG93 with red.

Sr/Ca ratios in the trabecular tissues than in the compact. The variation in Mg/Ca exhibits a much larger variation in both types of tissue in AG93. The turnover rate of trabecular tissue is generally higher than in compact bone tissue, and we propose that the variation seen could be due to differences in the concentrations of the bioavailable Sr, Ba and Mg at two (or more) locations where AG93 have lived, i.e. reflecting the provenance at two or more times in his life.



Apart from a low level ubiquitous environmental occurrence Hg was not widespread in the medieval societies. In analogy with the present day the major environmental source in the medieval time was probably fish in which Hg is biomagnified and present as methylmercury [5-7]. Besides this small environmental exposure Hg was mainly present in the form of occasional, distinct point

Figure 7 Aluminium in pg g-1 determined by ICP-MS in the bone samples of AG93 and AG104. The error bars represent ± 2 standard deviations of the analyticaluncertainty.

ct 30000

c <u o c o O

1 1 i i i i i i i M i m M i i AG 104 i i i i |ii 1 1 1 1 11 1 1 11 1 1 AG93 "

- • o Fe Mn

- i • f

- •• * 14 • • i • • • • •

• • • •

e©0©e® i i i i i i i i i @@@©©ee©e M M i i i M i e@ee@ e„@© © w ©e M i i M i i i i i i

Figure 8 Measurements of Fe and Mn. Iron (red points) and Mn (yellow points) in |jg g-1 for compact and trabecular tissue in both AG104 (left) and AG93 (right). The error bars represent ± 2 standard deviations of the analyticaluncertainty.

Figure 9 Measurements of Sr, Ba, Mg and Ca. Sr/Ca concentration ratios (green circles), Mg/Ca (red circles), and Ba/Ca (yellow circles) for AG104 (left) and AG93 (right). Squares representing the averages of 12 individuals from the Tirup cemetery (left) and 77 individuals from the Dominican Friary in Schleswig (right), in the same colour code as the circles. The average ratios from Tirup and the Dominican Friary in Schleswig are also listed in Table 1.The error bars represent ±2 standard deviations of the analyticaluncertainty.

sources, in contrast to for example Pb which was ubiquitous in the surroundings of all humans in medieval times, e.g. in the glazing of ceramics used for foodstuff, coins, roof tiles, window panes and even water pipes. The culturally defined potential point sources were: 1) the usage of Hg in medicine; 2) the use of cinnabar as a colour pigment in illuminating manuscripts and in paintings; 3) the application of gold onto other metals using amalgamation gilding; and finally 4) in the use of cosmetics (Vitruvius, book 7, chapter 8, section 4; Pliny the Elder book 29, chapter 8; [3-8]). In medieval Denmark the latter two must have been very rare, and we are left with main exposure ways either through medicine or through literary/academic work, both of which were mostly carried out in monastic environments [3].

Studies of modern day humans indicate that ingestion of Hg in individuals who are in Hg-equilibrium results in a body burden of approximately 100 times the daily intake, while the half-life of Hg in the body is about 70 days [9]. Mercury is excreted through faeces and urine. Over periods longer than 49 days the faeces account for 33-35% of the Hg excretion and the urine ca. 3.3% according to [10]. In a group of modern day non-exposed Japanese workers the Hg concentrations in the urine averaged 119 ^g L-1 [11]. In modern day humans the organic Hg (methylmercury) constitutes some 10-20% of the total Hg in most tissues, except in

the kidney and in hair where it is 2 and 63% respectively [5]. We have been unable to locate studies reporting the Hg speciation in the skeleton organ of modern man. Rasmussen et al. [3] concluded that the Hg concentration level in samples of compact femoral bone in non-exposed medieval and renaissance Danish individuals was between 10 and 100 ng g-1.

The large concentrations of Hg seen in the trabecular tissues are most likely not caused by contamination from the soil, as Rasmussen et al. [3] showed that there were no signs of diffusive processes in the Hg soil profiles they measured. Studying the details of the distribution of Hg in the two individuals investigated here two conclusions can be reached, namely that Hg is rather uniformly distributed in the compact bone tissue, and that Hg concentrations are generally higher in trabecular tissue and concentrated towards the abdomen region, as can be seen in two graphical displays in Figure 10. The central abdomen region is characterized by a large proportion of trabecular bone.

Two hypotheses can be presented as to what caused the higher Hg concentration values in trabecular tissue, extending up to 40 times the values of the compact bone tissue. The first hypothesis builds on the assumption that the Hg concentrations of the trabecular and the now decayed soft tissue were more or less similar and that the Hg content of the soft tissue was deposited onto the trabecular tissue upon decay of the body. During life

Figure 10 The Hg concentrations are depicted in colour codes for the two individuals. The colour code legend is shown to the left. Only the analysed bone elements are coloured. A concentration around the abdomen region is quite pronounced.

the trabecular tissue was intermixed with soft tissue -bone marrow, blood vessel, nervous tissue and fat - and if the Hg concentrations in the soft tissues were more or less similar to that of the compact bone tissue, then we underestimated the sample weight in our calculations. We have used the sample weight of the trabecular bone tissue preserved today, which is without the weight of the soft tissue now missing.

The second hypothesis is that the Hg is (or was) to a large degree present in organic form as methylmercury and therefore mainly present in the nervous tissue and the fat, rather than in the bone apatite. Should the Hg be present in bone apatite, it would likely be present in ionic form substituting for Ca2+. If the Hg was present in organic form it is likely that the Hg would concentrate in the soft tissues which are intermixed with the trabecular bone. After decay of the soft tissue the Hg will still be present inside the bone, only now collapsed onto the lamellae of the trabecular bone tissue making the overall concentration much higher than the compact bone value.

The first hypothesis can be brought to the test by estimating the original weight of trabecular tissue including the soft tissue by measurements of the bone compactness by CT-scanning. This has been done on a selection of the bones included in the present study. In the last column of Additional file 2: Table S1 is listed the measured compactness, k, and the Hg concentration corrected for this compactness. For AG104 such a correction brings the corrected Hg values quite close to the average Hg concentration in the compact tissue, RCt = 1.2 (test for RCt = 1: t = 0.96, df = 5, p = 0.19) where one sample is excluded as a very obvious outlier. The sample excluded is a sample of a vertebra that could easily be affected by proximity to inner organs which could accumulate Hg. However, even after applying the correction, the corrected Hg-values are still very high for the non-exposed individual AG93, RCt = 4.7 (test for RCt =1: t = 4.91, df = 4, p = 0.002), which is calculated excluding one anomalously high sample, again this sample originates from a bone surrounding the central cavity of the body, a rib. At face value the data of AG93 therefore contradicts hypothesis number one.

For elements not in equilibrium, RCt will differ from one. Tissue in the living human body undergo turnover as part of the process of remodelling and replacement. However, the turnover rate is not the same in all tissues. In general, turnover rates are much higher in soft tissues than in bones. Specific elements might accumulate in certain organs (like iodine in the thyroid gland). However, the way specific elements accumulate in different organs is not fully understood in all cases. There might therefore be a yet unknown reason why Hg may accumulate in the bones surrounding the abdomen. Bone turnover rates are also variable. The slowest turnover is

generally found in the compact tissue of the long bones [2]. Compact bone tissue in an area of major muscle attachments (like the linea aspera on the back side of the femur) probably has a substantively higher turnover rate than the surrounding compact femoral bone. Skeletal turnover rates are highest in trabecular bone [12,13]. Furthermore, in individuals where exposure to a given element has taken place mainly long before death RCt will be lower than one, as more substance will be retained in compact bone than in trabecular bone and soft tissue. In individuals where the exposure was highest close to the time of death RCt will be higher than one, as the concentration of the element will be highest in soft tissue and trabecular bone.

The RCt value of AG93, which at a first glance contradicted hypothesis number one, could therefore have another explanation. The high RCt value for AG93 could be explained if he was exposed to Hg only quiet late in life, i.e. shortly before his death. If such a scenario, where the distribution of Hg between compact and trabecular tissue is determined by the timing of the Hg-exposure, is viable then the Hg-exposure of AG104 must have happened at least some years prior to his death, whereas the low exposure of AG93 took place very late in his life. A background exposure late in life could for instance be caused by AG93 starting to eat fish late in life. This scenario, which can be termed chemical life history, is an alternative explanation to the two 'static' hypotheses presented above.

Judging from analyses of modern man it is unlikely that hypothesis number two can be the sole explanation, as methylmercury only constitutes about 10-20% of the total mercury in most tissue types [5].

Sites of attachment of muscles onto the bones are particular prone to remodelling of compact bone tissue. Such a site is linea aspera on the femur, where one of the large leg muscles adheres to the femur. We have analysed a sample of linea aspera in each skeleton, i.e. samples termed KLR8601-c9 and KLR8602-c9. As comparative material a sample was procured 90 degrees on either side of the femur (samples KLR8601-c10, KLR8601-c11, KLR8602-c10, and KLR8602-c11). In the non-exposed AG93 the three values c9, c10 and c11 are very similar (29, 31 and 20 ng g-1). However, in the Hg-exposed individual AG104 the sample of linea aspera, c9, is approximately two times higher than c10 and c11 (211, 119 and 124 ng g-1, respectively). This is an excellent observation showing how Hg is more easily incorporated into the compact tissue of linea aspera, if Hg is present in excess.


The issue of diagenesis is very complex indeed, and many different aspect of diagenesis is described in detail

by [14]. The particular case of possible diagenesis in connection with Hg was explored by [3], who measured horizontal and vertical soil profiles emerging from ca. 10 cm distally from caput femuris and 20 to 50 cm away from the body. These profiles were not consistent with diffusion profiles and it was concluded that no mobilisation of Hg took place on these scales, at least not in the three cases investigated from the cemetery in Tj^rby, Denmark.

We have extended the search for diagenetic processes by including studies of soil invading cavities and cracks in the bone and of groundwater depositing elements onto the bone. The invasion of soil can be traced by monitoring elements typical for clay minerals, as e.g. done by Fernandes et al. [15], who measured trivalent La, which substitutes for Al in clay minerals. In the present study we have analysed for Al itself, the concentrations of which is shown in Figure 7. As can be expected little or no soil invasion was observed in the compact bone tissue, as the Al concentrations the tra-becular samples were up to a factor of 50 higher than in the compact tissue. It makes no sense to impose a correction for the compactness in the case of Al, because there is no possibility of the majority of the Al to have originally been residing in the soft tissue. It is therefore inferred that elevated levels of Al must be due to soil invasion into the trabecular tissue. It should be noted that no decontamination were attempted during sampling of the trabecular tissue - it was simply not possible given the nature of the material, whereas the entire surface layer of the compact bones were removed prior to sampling. The samples of trabecular tissue were therefore analysed 'as found! which in some cases included invaded soil. In three cases, the trabecular part of the right femur of AG104, the trabecular part - right and left - of AG93, the Al concentrations determined were at the same low level of the compact bone samples (see Figure 7). We interpret this in the way that these three bone elements were intact and sealed, and that no soil had penetrated into the interior of these particular bones.

Besides soil invasion, diagenesis could also have taken place by seeping groundwater depositing previously dissolved ionic compounds. At some hydrological locations the groundwater carries large amounts of Fe and Mn [16-18], and the lay cemetery of Svendborg Franciscan Friary is such a place. The landscape rises steeply to the west of the friary, and the hillside running north-south here carries and releases a lot of groundwater. This was in all likelihood also the case in medieval times, as there was a water fountain ca. 10 m due west of the west wing which is discernible even today, and a relatively large water supply or sewer system in the brickworks of the Northern part of the west wing was observed during the 2007 excavation (Thomsen in prep).

The precise effect of the thorough mechanical decontamination of the compact bone tissue in the sampling phase was investigated by analysing the normally disposed outer surface tissue. This was done for one sample in each skeleton, KLR8601-c11u and KLR8602-c11u. Not unexpectedly the Al concentration is larger by a factor of ca. 10 and 20, respectively, as the outer surface of the bones have been in direct contact with the soil. What is more interesting is that the Hg in the surface layer is higher by factors of 16 and 19, respectively. This is surely not contamination from the environment, but most likely Hg from decomposed soft tissue deposited on the surface of the bone.

It must be expected that the trabecular tissue is more susceptible to groundwater deposition, as it has a vastly larger surface area exposed to the groundwater over the centuries, provided that the groundwater can indeed penetrate to the interior of the long bones. It is seen from Figure 7 that AG104 exhibits a uniform distribution of Mn with similar concentration values in the compact and the tra-becular tissues. The average Mn-concentration of both types of tissues in AG104 is 4340 ^g g-1. In AG93 there is, however, a pronounced difference between the compact tissues and the trabecular tissue samples, the trabecular types being higher in Mn. The compact bone samples in AG93 showed an average Mn concentration of 820 ^g g-1 and the trabecular 3360 ^g g-1. Taking the combined data into consideration it is possible that the bones of AG104 and AG93 have been subjected to the same flow of groundwater leading to a uniform deposition of Mn of approximately 4000 ^g g-1; only in AG93, it seems, has the groundwater penetrated into the compact bone tissues. It should be noted that the Mn-deposition seen in this study is anomalously high compared to other archaeological sites in the region. In the Dominican Friary in Schleswig in Northern Germany Mn-concentrations of ca. 30 ^g g- are normal and in individuals from the Tirup cemetery outside Horsens in Denmark concentrations of ca. 150 ^g g-1 are normally seen (unpublished results). These differences are undoubtedly caused by differences in the hydrological regimes of the cemeteries.

Iron is a factor of 2 elevated in the trabecular samples in AG104 relative to the compact samples (average Fe: compact 11,820 ^g g-1 and trabecular 23,630 ^g g-1). For AG93 the trabecular tissue is on average 8 times higher in Fe concentrations - indicating that groundwater deposition of Fe over the years has been much more severe for this skeleton.

However, neither soil invasion nor groundwater deposition of Fe and Mn can be seen to have influenced the other elements analysed in samples of decontaminated compact bone samples, and it seems unlikely that the action of the groundwater have changed the concentrations of Hg, Ba, Sr or Mg in the skeletons.


Much attention has been given recently to Sr-isotopes as an indicator of provenance (e.g. [19]). However, the ratios Mg, Sr and Ba to Ca have also been utilized to monitor differences in provenance (e.g. [20-22]). The Mg/Ca ratio shows some relatively large variations, whereas the Sr/Ca and the Ba/Ca ratios show less variation. The compact bone samples yield less variation than the trabecular samples. The trabecular tissue has the highest turnover rate and is therefore indicative of the provenance late in the life of the individuals. The best estimate of the provenance of Svendborg medieval town is therefore approximated by the following average ratios calculated from the trabecular bone tissues: Sr/Ca = 27.3 ± 2.1, Ba/ Ca = 30.0 ± 3.2 and Mg/Ca = 41.8 ± 4.2 (for AG104); and Sr/Ca = 18.2 ± 6.2, Ba/Ca = 31.5 ± 10.1 and Mg/Ca = 49.3 ± 15.3 (for AG93).

The compact bone averages are: Sr/Ca = 22.8 ± 2.5, Ba/ Ca = 21.1 ± 2.7 and Mg/Ca = 42.6 ± 2.9 (for AG104); and Sr/Ca = 7.7 ± 1.4, Ba/Ca = 5.4 ± 1.6 and Mg/Ca = 91.5 ± 14.5 (for AG93). In the lower left corner of Figure 9 squares indicate the averages of the Ba/Ca, Sr/Ca and Mg/Ca ratios measured on samples of various compact and trabecular bones from 77 individuals interred at the Dominican Friary in Schleswig, Germany, and 12 such samples from Tirup cemetery outside Horsens, Denmark (unpublished data). It is seen that the compact bone values from AG93 are similar to Tirup and Schleswig in Sr/Ca and Ba/Ca ratios, but very different in Mg/Ca ratios. AG104 is altogether different from Tirup and Schleswig in all three ratios.

It seems likely that AG93 was a migrant, who had lived for a prolonged time at some other place than Svendborg where he was eventually buried. AG104, on the other hand, seems to have been stationary in Svendborg throughout his relatively short life.

Other elements

The rest of the elements, Zn, As and Pb, show little variation in the samples analysed as can be seen by the factor loadings shown in Figure 2. It is possible and even likely that Zn, As and Pb have all been substituting for Ca in the bone apatite. The fact the Zn seems not to exhibit any large systematic variation is consistent with Zn being strongly controlled by metabolic processes in the body. There are no indications that As and Pb are strongly metabolically controlled elements, and the explanation for their lack of variation may simply be that these elements have been more or less homogenous in the food and environment of AG93 and AG104.


We have measured Mg, Al, Ca, Mn, Fe, Zn, As, Sr, Ba, Hg and Pb in 73 bone samples from two medieval individuals

interred in the laymen cemetery of the Franciscan Friary of Svendborg. The trace element concentrations of the two individuals can be clearly distinguished by Principle Component Analysis, which also show a clear distinction between compact and trabecular bones tissues.

Mercury is almost uniformly distributed in all compact bone elements sampled in one individual. In the tra-becular tissue Hg occurs in much higher concentrations than in compact bone. This can be due to either of two 'static' hypothesis: 1) that there were more less similar Hg concentrations in bone and soft tissues and that the original sample weight was underestimated in the tra-becular bone tissue due to the loss of soft tissue; 2) that Hg was present in organic form and therefore mainly concentrated in the nervous tissue and fat in the bone marrow and that the Hg content of this decomposed tissue is now deposited onto the surface of the bone lamellae of the trabecular tissue. We argue that our data are in support of the first hypothesis, combined with high Hg exposure several years before death for AG104 and a low exposure towards the end of life for AG93. Our data therefore reflect what can be termed a chemical life history of the individuals.

If excess levels of Hg were present in the body it is more likely to be incorporated in compact bone tissue at sites of vivid remodelling, as has been demonstrated for linea aspera on the femur of AG104.

There are good indications from the present data and the literature that Sr/Ca, Ba/Ca and Mg/Ca can be used to reveal the provenance of the individual through life. Large differences can be seen in these elemental ratios for medieval individuals from Svendborg, Tirup and Schleswig. The elemental ratios differ between the two individuals investigated here, AG104 exhibits more or less constant ratios for all bones and he has therefore in all likelihood lived his entire life in Svendborg, while AG93 seems to have lived part of his earlier life somewhere else.

Diagenetic processes are reflected in the concentrations of Al, Fe and Mn. Soil invasion can be monitored by measuring Al, typically present in clay minerals. Groundwater seems to have deposited Fe and Mn onto and to some degree into the bones of the individuals interred at the Franciscan Friary of Svendborg.

Additional files

Additional file 1: Franciscan Friary Svendborg Denmark.

Additional file 2: Table S1. Results of the elementalanalyses by CV-AAS (Hg) and ICP-MS (allother elements). First column: Laboratory numbers. Second column: tissue type. Third column: sample weight in grams. Second-next-to-last column: Compactness measured by CT-scanning (k in formulae 2). Next-to-last column: corrected Hg concentration (Ct-k from formula 3). Last column RCtk (from formula 3) - the ration between the corrected Hg concentration (Ctk) and the average compact bone Hg concentration (Cc).

Competing interests

The authors declare that they have no competing interests. Authors' contributions

LS, CP and AL performed various parts of the chemistry. KLR drafted the manuscript. PML performed the CT-scanning. JLB performed the anthropological examinations. Allauthors read and approved the final manuscript.


This work was supported by EU Interreg 4a through the project Bones4Culture. Pia Klingenberg Haussmann is thanked for technicalassistance in this project. Two anonymous referees are thanked for constructive criticisms.

Author details

institute of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej55, Odense M DK-5230, Denmark. 2Institute of Forensic Medicine - ADBOU, University of Southern Denmark, Lucernemarken 20, Odense S DK-5260, Denmark. 3Arteologi Sydfyn, Grubbem0llevejl3, Svendborg 5700, Denmark.

Received: 28 February 2013 Accepted: 22 March 2013 Published: 6 April 2013


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Cite this article as: Rasmussen et al.: The distribution of mercury and other trace elements in the bones of two human individuals from medieval Denmark - the chemical life history hypothesis. Heritage Science 2013 1:10.

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