Scholarly article on topic 'OL-033 Clinic observation and analysis of congenital syphilis (12 cases)'

OL-033 Clinic observation and analysis of congenital syphilis (12 cases) Academic research paper on "Clinical medicine"

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Academic research paper on topic "OL-033 Clinic observation and analysis of congenital syphilis (12 cases)"

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|QL-030| Synergistic interplay between Helicobacter pylori virulence genes and host COX-2 and iNOS enhances the risk of premalignant and malignant lesions S.K. Tiwari*, G. Manoj, G. Sivaram, R. Saikant, Z. Abid, Md. Aejaz Habeeb, A.A. Khan, C.M. Habibullah. Center for Liver Research and Diagnostics, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad 500058, Andhra Pradesh, India

Background and Aim: H. pylori (Hp) strains vary in their carcinogenic potential. Both host factors and bacterial factors have been postulated to contribute to the variable outcome. Over expression of host COX-2 and iNOS has been implicated in the development of gastric carcinoma. Furthermore, the link between genotypes in relation to COX-2 and iNOS expression and disease status needs to be determined. Therefore the present study addressed to identify Hp-bearing hosts who are at greatest risk of developing precancerous lesions.

Methods: A total of 240 subjects with various gastric disorders were screened. Genotyping based on cagA, cagE, cagT, vacA signal region and hrgA genes of Hp was performed using DNA from gastric biopsies. Expression of COX-2 and iNOS was assessed by Rt-PCR and immunoblotting. Histological scoring of antral and corpus biopsies for the presence precancerous lesions was done. Results: The genotype cagA+/cagE+/cagT+/hrgA+/vacAs1 showed high prevalence 177 (73.7%). Among which 81.1% had overt gastric disorders whereas 46% subjects had less severe gastric disease. Histology revealed presence of atrophy in 52% vs 18%, IM in 32% vs 9% and dysplasia in 20% vs 4% respectively (Statistically significant at p < 0.01). RT-PCR and immunoblotting data showed high expression patterns of COX-2 and iNOS in overt gastric disorders than with less severe gastrointestinal disorders. Conclusion: Genotype cagT+ve/hrgA+ve/cagA+ve/cagE+ve/ vacAs1+ve and heightened expression levels of COX-2 and iNOS have higher differentiating and predictive value for the development of severe disease manifestations. This suggests that Hp induced gastric inflammatory reaction to be influenced by multiple factors, and probably results from the synergistic effect of bacterial virulence and host factors, which work together in a complex way causing various diseases in the host.

|OL-O311 Pediatric Helicobacter pylori gastritis: endoscopic and pathologic analysis

H.S. Kim1*, H.S. Rhee2, J.K. Seo3. 1 Department of Pathology, Inje Univ. Ilsan Paik Hospital, Goyang city, South Korea, 2Rhee Hi Soo Pediatric Clinic, Seoul, South Korea,

3Department of Pediatrics, Seoul National Universith Hospital, Seoul, South Korea

Background and Aims: Although Helicobacter pylori gastritis is a worldwide problem, the study concerning pediatric patients seems to be rare. The aim of this study was to investigate the characteristics of childhood Helicobacter pylori gastritis by the endoscopic and histopathologic analysis.

Methods: Eighty children with gastritis were enrolled in this study. Forty children were H. pylori positive and another 40 children were H. pylori negative. The endoscopic findings and their histopathologic features were analyzed. Results: The grade of H. pylori density was positively correlated with the degree of mononuclear cell infiltration (p = 0.039). The mucosal erosion was significantly lower in young age group (<10) than in old age group, especially in H. pylori grade 3 group (p = 0.04). Endoscopical analysis shows 80% of H. pylori positive cases had nodular gastritis.

However, only 15% had nodular gastritis in H. pylori negative cases. Endoscopically proven nodular gastritis reveals positive correlation with lymphoid follicles and mononuclear cell infiltration (p < 0.001). Conclusions: This study reveals that pediatric H. pylori gastritis had remarkable histopathologic reactions (such as mononuclear cell infiltration, lymphoid follicles, mucosal erosion) and endoscopically proven nodular gastritis compared to non-H.pylori gastritis children. The mucosal erosion seems increase significantly in elder age group (>11 years) than younger group (<10 years) of H.pylori positive gastritis children.

|QL-032| Emergence of avian influenza in Bangladesh:

factors, consequences and policy options M.M. Islam*. Institute of Population Research, Peking University, Beijing, 100871, China

Background: Nowadays pandemic influenza is receiving the most attention in the resurgence of infectious diseases as it has emerged as a global threat by causing extensive morbidity and mortality in the world. The continuing threat of avian influenza by risk and preparedness has made the Asian continent more vulnerable where the mechanisms involved in the emergence of influenza virus and the epidemiological factors leading to pandemics are still unpredictable. In this respect the influenza epidemics in Bangladesh due to its agricultural-based communities and highest population density can be spread out more where its demographic, economic and other impacts will be more significant. However, the subsequent risk for generating a pandemic human strain is still unknown. More academic research is needed to understand the factors, possible consequences with appropriate policy options to resist such pandemic influenza.

Objectives: The overall objective of the study is to examine the evolution or transition of pandemic influenza in Bangladesh with emphasis to avain influenza (H5N1) by identifying the factors, consequences and policy alternatives regarding its potential to cause the next pandemic.

Methodology: The proposed study is an explorative activity in nature Therefore various interdisciplinary (public health, sociology, demography, economics, anthropology, history etc) approaches, techniques and methods are expected to be used for this research.

Data and its availability: Available secondary character of data at different levels (e.g. international, national, local or institutions etc) that permits a comprehensive analysis regarding pandemic influenza in Bangladesh is expected to be applied.

|QL-033| Clinic observation and analysis of congenital

syphilis (12 cases) Y. Gu*, L. Pang, H.H. Zeng. Pediatric department of Beijing Ditan Hospital, Beijing, 100015, China

Congenital syphilis is a chronic contagious disease which caused by mother to baby transmission. As the ascending morbidity of syphilis in these years, the incidence of congenital syphilis increased gradually. In this article data of 12 congenital syphilis cases in our hospital were collected and analysed since 2002. The main manifestations include fever, skin lesion, jaundice and hepatosplenomegaly. In addition, bone abnormality was found in 2 cases, and hematology damages were exhibited by anemia (5 cases) and by thrombocyte reducing (3 cases). All the cases are in response to penicillin. 3 cases with neurosyphilis have nondifferential neural symptoms, and the abnormal cytology, plasma reagin test (RpR) and Treponema pallidum particle

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agglutination (TPPA) of cerebrospinal fluid (CSF) help to diagnose. These cases recovered after 3 weeks' therapy with penicillin. Myocardial damages and hepatic injuries were observed in 3 cases complicating cytomegalovirus (CMV) infection, and ganciclovir therapy is responsive. 1 case with mass proteinuria and edema didn't undergo kidney biopsy. In conclusion, the initial symptoms of congenital syphilis are always single and unspecific, however, the manifestations are various with multi-organ injury, and laboratory findings help to diagnose. As an effective means of therapy, penicillin is of choice.

|QL-034| Research of T-cell subsets of

hand-foot-and-mouth disease (HFMD) H.H. Zeng*, Y. Gu, Z.H. Chen. Infection Department of Beijing Ditan Hospital, Beijing, 100015, China Objective: To evaluate the dynamic changes of T-Cell Subsets in children with hand, foot and mouth disease to provide new evidence for the therapy and prognosis. Methods: Two peripheral venous blood samples of 76 HFMD cases in acute stage and in recovery time of HFMD were collected. T-Cell Subsets were assayed by flow cytometer. Results: (1) The dynamic changes of T-Cell Subsets: the Th and T lymphocytes/lymphocytes of all cases step-up in recovery time of diseases (p < 0.05). General cases had the same pattern, every index of severe case have increased apparently (p <0.05). (2) The differentiation of T-lymphocyte subsets of general cases and severe cases: Th and T-lymphocytes and lymphocytes of pathoformic severe cases is lower than which in general cases (p < 0.05). In recovery time, there is no differentiation of T-Cell Subsets in severe cases and general cases (P >0.05). (3) The relationships of T-Cell Subsets and Length of Stay: Th/lymphocyte is correlated to Length of Stay in some degree.

Conclusions: The insufficient cellular immune function is the reason of childrens affectability to HFMD, the magnify cellular immune function and state of an illness is at equal pace; T-Cell Subsets can warn the course of disease in some degree.

|QL-035| Assessing health-related quality of life of liver transplantation recipients

Y. Liu*, L. Jiang, Y. Mu. The surgery department of Beijing Ditan Hospital, Beijing, China

Objective: To assess pretransplantation and posttransplantation health-related quality of life (HRQL) of liver transplant recipients.

Methods: HRQL was assessed at several timepoints using the Medical Outcomes Short Form 36 (SF-36). During January 2004 to December 2006, 34 individuals selected to receive treatment at the surgery department of Beijing Ditan hospital in China were eligible to be included. Results: A paired comparison of HRQL at listing and 3 months posttransplantation showed statistically significant improvements (P < 0.05) in all dimensions of the SF-36, except for Bodily Pain [P>0.05]. An analysis of posttransplantation HRQL over time for patients who survived until 24 months posttransplantation showed a statistically significant improvement (P < 0.05) for all dimensions of the SF-36 (apart from Mental Health [P = 0.083 > 0.05] and Rol^Emotional dimensions[P = 0.105 >0.05]). Several dimensions of the SF-36 were found to be associated with a number of pre-transplant factors: age, disease severity (classified by Child Pugh class), and liver transplant history (whether the patient had received a single or multiple transplants). Conclusion: Liver transplantation could significantly improve quality of life (QOL) of liver transplant recipients. The model for end-stage liver disease (MELD) score was not

predictive of QOL. In making clinical decisions about the use of transplantation, consideration should be given to the key factors likely to affect subsequent health-related quality of life.

Table 1. SF-36: paired comparison transplantation patient profiles

of listing and 3-month post-

Dimensions Mean score (n = 34) t-Statistica P

Listing 3 months post-Tx

PF 39.12±19.09 51.76±9.12 -3.413 0.001

RP 0.02±19.35 20.00±10.15 -4.718 <0.001

BP 63.24±22.01 56.76±10.07 -1.391 0.164

GH 25.59±18.82 59.85±10.19 -6.240 <0.001

VT 39.12±26.53 54.56±11.77 -3.225 0.001

SF 38.75±24.07 60.59±10.99 —4.340 <0.001

RE 32.16±35.02 57.97±12.37 —4.015 <0.001

MH 52.26±18.67 66.32±9.32 -3.765 <0.001

at-test; P< 0.05.

Table 2. SF-36: paired comparison of 3-, transplantation patient profiles

6-, 12- and 24-month post-

Dimensions Post-Tx mean score (n = 34) c2a P

3 months 6 months 12 months 24 months

PF 51.76 59.59 78.68 79.12 59.420 <0.001

±9.12 ±7.40 ±17.68 ±24.91

RP 20.00 30.71 78.97 78.82 80.849 <0.001

±10.15 ±10.97 ±21.03 ±27.00

BP 56.76 62.35 78.75 77.43 38.273 <0.001

±10.07 ±11.23 ±19.18 ±25.18

GH 59.85 61.03 71.32 62.21 8.300 0.040

±10.19 ±10.28 ±25.71 ±25.94

VT 54.56 58.24 74.85 69.41 33.490 <0.001

±11.77 ±10.72 ±21.30 ±24.27

SF 60.59 69.12 75.66 75.59 28.236 <0.001

±10.99 ±9.25 ±23.86 ±29.21

RE 57.97 57.82 61.67 64.56 6.143 0.105

±12.37 ±12.19 ±30.08 ±39.62

MH 66.32 66.32 72.18 67.38 6.684 0.083

±9.32 ±9.32 ±22.66 ±25.48

aChi-square test; P< 0.05.

|QL-036| Oxidative stress, immune dysfunction and inflammatory responses in benzene workers

B. Santhosh Kumar1*, S.K. Tiwari2, G. Manoj2, U. Nazia1, R. Devender Reddy1. 1 Department of Physiology, Deccan College of Medical Sciences, Hyderabad-500 058, India,

2Center for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad-500 058, India Background and Aim: Benzene and its derivatives form active ingredients of petroleum solvents. Continuous exposure to these toxic metabolites is known to be hazardous as they drastically cause cytotoxicity, immunotoxicity, DNA damage and carcinogenesis. Professional petrol filling workers are at an increased risk to suffer with multitude of diseases. The present study therefore evaluated the oxidative stress and immune dysfunction among professional petrol filling workers continuously exposed to benzene and its derivatives. Methods: A total of 78 subjects (48 professional workers and 30 healthy volunteers) were recruited. Oxidative stress markers namely ROS, lipid peroxides, detoxifying agents such as glutathione and antioxidants were measured in blood samples collected from these subjects. Expression of p53 and COX-2 was also evaluated using reverse transcriptase polymerase chain reaction. The levels of immune cells like CD4+, and antibodies like IgG1 and IgG2 were measured to evaluate the immune status.

Results: Levels of ROS and lipid peroxides were elevated among the professional workers than the controls indicating