Scholarly article on topic 'The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography'

The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography Academic research paper on "Biological sciences"

Share paper
Academic journal
Sci. Rep.
OECD Field of science

Academic research paper on topic "The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography"


OPEN The room temperature

crystal structure of a bacterial phytochrome determined by serial

Received: 25 May 2016 Accepted: 15 September 2016 Published: 19 October 2016

femtosecond crystallography

Petra Edlund1'*, Heikki Takala1-2-3-*, Elin Claesson1, Leocadie Henry1, Robert Dods1, Heli Lehtivuori4, Matthijs Panman1, Kanupriya Pande5, Thomas White5, Takanori Nakane6, Oskar Berntsson1, Emil Gustavsson1, Petra Bath1, Vaibhav Modi7, Shatabdi Roy-Chowdhury8, James Zook8, Peter Berntsen9, Suraj Pandey10, Ishwor Poudyal10, Jason Tenboer10, Christopher Kupitz10, Anton Barty5, Petra Fromme8, Jake D. Koralek11, Tomoyuki Tanaka12, John Spence13,14,15, Mengning Liang11, Mark S. Hunter11, Sebastien Boutet11, Eriko Nango12, Keith Moffat16,17, Gerrit Groenhof7, Janne Ihalainen18, Emina A. Stojkovic19, Marius Schmidt10 & Sebastian Westenhoff1

Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 A resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 A resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.

Phytochromes sense ambient light levels in plants, fungi, and bacteria. The proteins detect light and trigger intracellular signalling cascades, which regulate many light-dependent phenotypes. Examples include shade avoidance and seed germination in plants1'2, the control of the abundance of photosynthetic proteins in cyanobacteria3, and

■"■Department of Chemistry and Molecular Biology, University of Gothenburg, 40530 Gothenburg, Sweden. 2Department of Biological and Environmental Sciences, University of Jyvaskyla, 40014 Jyvaskyla, Finland. 3Faculty of Medicine, Anatomy, University of Helsinki, 00014 Helsinki, Finland. 4Nanoscience Center, Department of Physics, University of Jyvaskyla, 40014 Jyvaskyla, Finland. 5Center for Free-Electron Laser Science, Deutsches ElektronenSynchrotron DESY, D-22603 Hamburg, Germany. 6Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan. 7Nanoscience Center, Department of Chemistry, University of Jyvaskyla, 40014 Jyvaskyla, Finland. 8School of Molecular Sciences, Arizona State University, 85287 Tempe, Arizona, USA. 9Australian Research Council Centre of Excellence for Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, 3086 Melbourne, Victoria, Australia. 10Physics Department, University of Wisconsin, WI 53211 Milwaukee, USA. 11Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA. 12RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, 679-5148 Hyogo, Japan. 13Biodesign Institute, Arizona State University, 85287 Tempe, Arizona, USA. 14Center for Applied Structural Discovery, Arizona State University, 85287 Tempe, Arizona, USA. 15Department of Physics, Arizona State University, 85287 Tempe, Arizona, USA. 16Department of Biochemistry and Molecular Biology, University of Chicago, 60637 Chicago, Illinois, USA. 17BioCARS, Argonne National Laboratory, 60439 Argonne, Illinois USA. 18Nanoscience Center, Department of Biological and Environmental Sciences, University of Jyvaskyla, 40014 Jyvaskyla, Finland. 19Department of Biology, Northeastern Illinois University, 5500 North St. Louis Avenue, 60625 Chicago, Illinois, United States. *These authors contributed equally to this work. Correspondence and requests for materials should be addressed to M.S. (email: or S.W. (email:

Figure 1. Comparison of the crystal structures for the PAS-GAF fragment from bacteriophytochrome from D. radiodurans at cryogenic (LowT) and room (SFX) temperatures. The datasets are truncated at 2.1 Â resolution in all panels. (a) Superimposed SFX (green) and LowT (grey) structures plotted together with the Fo(SFX)-Fo(LowT) difference map (red: negative, green: positive) at 3.8 ct. The overall R-factor for difference map is 24.5%. (b) The same representation zoomed into the chromophore region. The Fo(SFX)-Fo(LowT) difference map is contoured at 3.0 ct. (c) The LowT structure and the corresponding maps (2Fo-Fc, blue at 1.3 ct and Fo-Fc difference map at 3.3 ct). The extra water only present in the LowT structure is indicated by a red arrow. ( d) The SFX structure and the corresponding maps (2Fo-Fc and Fo-Fc maps, contour levels as in c).

the control of carotenoid expression in bacteria4. In bacteria, the proteins often function as histidine kinases in a two-component signalling system5.

Phytochromes share a modular domain architecture. The widely conserved photosensory core module usually consists of PAS (PER, ARNT, SIM), GAF (cGMP phosphodiesterase, adenylate cyclase, FhlA), and PHY (Phytochrome-specific GAF related) domains5. The PAS and GAF domains hold a tetrapyrrole bilin chromophore, represented by phytochromobilin, phycocyanobilin, or biliverdin in plants, cyanobacteria, or bacteria, respectively. The chromophore is covalently attached via a thioether linkage to a conserved cysteine in either the PAS domain (bacteria) or GAF domain (cyanobacteria and plants) (Fig. 1).

Upon light absorption by the chromophore, a sequence of structural changes is initiated, which shuttles the protein from a red light-absorbing state (Pr) to a far-red light-absorbing state (Pfr) or vice versa. These structural changes involve at least a Z-to-£ isomerization of the C15 = C16 double bond of the chromophore with associated changes of the chromophore-binding pocket6-9 and refolding of the so-called PHY-arm6,7,10,11. It is unclear how these changes extend from the photosensory core into the output domains. Furthermore the details of the isomerization reaction and the sequence of how the conformational changes arise remain poorly understood.

Conformational heterogeneity of the chromophore has been suggested to play an important role in the photocycle. Two Pr-state conformations have been detected for a cyanobacterial phytochrome in solution with NMR spectroscopy12. This finding is contrasted by the crystal structures of phytochromes, which only show one chromophore conformation6,7,13,14, at resolutions approaching 1 A (this work and ref. 9). It is not clear whether the crystal packing, or cryogenic temperature at which crystallographic data are recorded, favours only one conformation, or whether the crystal structures truly reflect the solution structures.

SFX is an attractive method for resolving the structural changes in light-activated proteins15. In SFX, diffraction data are collected from a stream of microcrystals in a liquid jet flowing across the X-ray focus. Data from thousands of individual microcrystals, each exposed only to a single femtosecond X-ray pulse, are sorted, indexed, and merged into one complete set of reflection intensities16,17. Thus it becomes possible to expose the crystals to an optical laser pulse prior to data collection. Structural snapshots of the protein at defined stages of the photoreaction can be recorded15,18. In this paper we report the crystal structure for the chromophore-binding domains (PAS-GAF) of Deinococcus radiodurans recorded by SFX. Crystallographic data are collected at room temperature and the crystals are not subject to significant radiation damage at our resolution19,20.

Wavelength (nm)

Figure 2. Microcrystallisation of PAS-GAF. (a) Micrograph of microcrystals produced by direct crystallisation in microcentrifuge tubes, and (b) the microcrystals produced by crushing macrocrystals (inset) with seed beads. (c) Absorption spectra of PAS-GAF in solution (black) and microcrystals (blue). (d) Structure and atom names of the bilverdin cofactor.


Crystallisation and data collection. Modified crystallisation conditions were developed to yield good quality macrocrystals. The PAS-GAF fragment of the D. radiodurans phytochrome was crystallised in essentially the same conditions as reported elsewhere13, but the cryoprotectant 2-methyl-2, 4-pentanediol was added directly to the crystallisation buffer (see methods). Those changes resulted in the protein packing as monomers instead of dimers in contrast to previously published conditions13. The space group C121 and cell dimensions were highly similar to the crystals from monomeric PAS-GAF21. We recorded diffraction data at cryogenic conditions at the European Synchrotron Radiation Facility (ESRF) and solved the structure to 1.35 A resolution by molecular replacement with 4Q0H9 as a search model. We refer to this structure as "LowT".

A protocol for the preparation of microcrystals suitable for SFX was developed based on the conditions described above. The microcrystals were grown in batches of 0.5 mL in a microcentrifuge tube at 10 degrees Celsius (see methods). The typical crystal size was 5-10 |im with a narrow size distribution as judged by visual inspection with a microscope (see Fig. 2a). Figure 2c compares the absorption spectra of the protein in solution and in microcrystal suspensions. The microcrystal spectrum is highly similar to soluble one, except for a 5 nm red shift and a small shoulder at the far-red part of the 700 nm peak (Q band).

To record SFX data from these crystals, the suspensions were injected into a vacuum chamber using a gas dynamic virtual nozzle22 at the CXI instrument of the Linac Coherent Light Source (LCLS)23. The stream of crystals was exposed to femtosecond X-ray pulses of approximately 1.8 mJ/pulse at 9.5 keV. 114409 hits were identified using Cheetah24 and 90428 frames were indexed and merged using CrystFEL version 0.6.1 + c9c725. The structural model was solved using Phaser with 4Q0H9 as a search model. We refer to this structure as "SFX".

Comparison of room temperature and cryogenic structure. Figure 1 compares the LowT (PDB code 5K5B) and SFX (PDB code 5L8M) structures. For the SFX structure, X-ray data were recorded at room temperature and appear essentially free of radiation damage at our resolution. This is a consequence of the "diffraction before destruction" principle, where each crystal is exposed only once to a single X-ray pulse with femtosecond duration19. Conventional crystallography is typically performed at cryogenic temperatures and changes may occur upon cooling26. Also, radiation damage typically occurs, depending on the X-ray dose. Overall, we find

Figure 3. Comparison of LowT and SFX structures to earlier published PAS-GAF structures. (a)

Comparison of the biliverdin conformation and cystein24 thioether linkage of LowT and SFX structures with previously reported PAS-GAF structures 4IJG21, 4Q0H9, and the PAS-GAF-PHY structure 4Q0J9. 4Q0H has very similar conformation around the thioether bond compared to 2O9C and 2O9B8, which are not displayed for clarity. (b) Comparison of the SFX structure and 4Q0H9 PAS-GAF-structures reveals differences in the D- and A-ring orientation, the thioether linkage and the positions of Tyr263, Met267, Tyr173 and the residues 17-25 and 184-186. The grey water (marked by red arrow) is only observable in the LowT structure (see text for details).

excellent agreement between our structures at cryogenic and ambient temperatures. Figure 1a shows the difference electron density (Fo(SFX) - Fo(LowT)) at 3.8 a contour level for the whole protein. The positive and negative peaks are mostly localized to the outer parts of the globular domains. This is expected, since thermal motions are likely to be more significant at these positions. However, very few difference density peaks are observed on the backbone atoms and on the chromophore, indicating close agreement between the two structures.

Figure 1b demonstrates the Fo(SFX) - Fo(LowT) map of the biliverdin chromophore and its close surroundings. At 3a the only associated positive and negative electron densities are observed close to the thioether bond of the chromophore. This indicates a different position of the thioether linkage in the LowT compared to the SFX structure. It has been shown that flash-cooling can introduce packing and local structural perturbations and it is likely that the differences in the Fo maps reflect such effects26. Moreover, this part of the structure is not uniquely resolved in the LowT data, and the associated negative peak are observed in the Fo-Fc maps (Fig. 1c). However, the Fo-Fc map of the SFX structure (Fig. 1d) does not indicate any discrepancy between data and model and a unique covalent bonding geometry was refined. The diffuse electron density in the LowT structure (Fig. 1c) could be due to radiation damage or to alternate conformations of the chromophore-protein bond. In any case, our SFX structure does not show this ambiguity. The SFX structure confirms, to a resolution of 2.1A, that a single chromophore geometry is present in the crystals.

We also note that the LowT data support a water molecule with partial occupancy close to Tyr263 (arrow in Figs 1c and 3). The water is hydrogen-bonded to the A-ring carbonyl, the pyrrole water and the functionally important Asp207. The electron density from SFX data did not support the modelling of the water molecule. We postulate that this water either exchanges rapidly with the solvent surroundings or that it is expelled by higher overall thermal motion of the surrounding atoms at room temperature.

Structural differences and chromophore structural properties. In Fig. 3 our structures are compared to other PAS-GAF structures. On the one hand, our structures agree well with structures of a monomeric variant (4IJG) of PAS-GAF, which has three mutations to block dimerization (Fig. 3a)21. On the other hand, the positions of the backbone atoms, the chromophore and its binding pocket differ from a certain group of structures. These structures originate from crystals formed of proteins with a mutation (Y307S) to aid crystallisation which, by altering the crystal contacts, results in a different crystal packing (see Fig. 3a,b)8,9.

In particular, we found that the D-ring of the biliverdin chromophore is more planar in our structures (dihedralN09,C08,C06,C04 = 64.8 degrees compared to 73.4 degrees in 4Q0H, see Fig. 2d for biliverdin nomenclature) and that the Tyr263 and Tyr176, which sandwich the D-ring, adjust accordingly (Fig. 3b). From Tyr176 these structural differences translate to a slight upward shift of the backbone of residues 184-186. Tyr263 shifts away from the pyrrole water, which also makes room for the extra water between the pyrrole water and the phenol ring, which is observed by electron density in the LowT structure (Fig. 1c). Furthermore, Met267, which is a direct

Figure 4. Structural overlay of the SFX (green) and SACLA (sand) structures plotted together with the Fo(SFX)-Fo(LowT) difference map (red: negative, green: positive) at 3.8 ct. (a) data truncated at 2.2 Â (overall R-factor 14.6%) and (b) data truncated at 2.5 Â (overall R-factor 11.6%).

neighbour of Tyr263 appears in a different position. The density for this residue appears diffuse in our LowT structure (data not shown) and the same residue has been modelled by others with multiple conformations9. In the new SFX structure, the electron density for this residue does not support multiple conformations; hence the diffuse electron densities in the LowT structures may arise from radiation damage. Structural differences between the new structures and 4Q0H are also seen in the residues flanking Cys24 and the thioether linkage (Fig. 3b). In fact, many published wild-type PAS-GAF structures show different conformations of the Cys24 and the A-ring of the chromophore, indicating enhanced conformational flexibility in this region (Fig. 3a).

Method and structural results are enforced by additional experiment. Finally, we report a second method to generate micron-sized phytochrome crystals for performing SFX experiments. To this end, macro-crystals were crushed with seed beads by vortexing in a microcentrifuge tube. Microscope images revealed that the crystal slurry had a broad size distribution even after filtering through a 20 |im filter and contained crystals sizes ranging from 5 |im to 30 |im (see Fig. 2b). These sets of crystals were dispersed in grease27 and exposed to short pulses of X-rays at 7 keV at the SPring-8 Angstrom Compact free electron LAser (SACLA). 30146 hits were recorded in 3 hours of beamtime of which 20014 were indexed using CrystFEL25. The structure shows overall good agreement with the other structures reported in this paper (see Fig. 4). When comparing the difference electron density map (SFX-SACLA) at 2.5 A resolution, only very few difference peaks are observed (Fig. 4b). At higher resolution the data recorded at SACLA starts to diverge from the SFX data set (Fig. 4a). This is likely due to the fact that less frames with high resolution data were recorded at SACLA, which can be traced back to the broad size distribution of the crystal preparation.


Here we report the crystal structure in a new crystal form of the phytochrome PAS-GAF domains from D. radio-durans. Compared to other structures of the same protein fragment, these crystal conditions did not require mon-omerizing mutations28 or mutations to stabilise the crystal packing8,9. The 1.35 A resolution to which the LowT structure has been determined is the highest reported so far for a wild-type PAS-GAF fragment. Importantly, the LowT structure corroborates the SFX structures. The comparison is very direct, because the crystallisation conditions for the SFX and conventional crystallography were identical (SACLA) or highly similar (LCLS). More generally, this implies that a large body of structural conclusions, which were drawn from crystal structures obtained at cryogenic temperatures, are also valid at ambient conditions6,7,9,10,14,29.

Except for the ambiguity in the thioether bond in the LowT data, the SFX and LowT data support only one chromophore conformation (Fig. 3 a). This is further confirmed by composite omit maps (Fig. 5). This finding is in agreement with other reported structures, which all found only one conformation6-9,13,30. However, the conformation of the chromophore and its thioether linkage reported here differs from the previously solved structures of the same phytochrome fragment (Fig. 3)8,9,13. These crystals had a different packing and thus the differences in conformation are most likely introduced by packing effects. The structures presented in this paper confirm that the chromophore and protein can, in principle, adopt an alternative conformation. It is therefore likely that the protein in solution adopts, at least, both conformations. These findings are in qualitative agreement with the previous reported results that a cyanobacterial phytochrome in Pr exists in at least two conformations12 and that the spectroscopic evolution supports heterogeneous conformations31.

In order to record SFX data, the microcrystals have to be delivered to the beam. There are currently two main approaches to achieve this. In one method, a buffer solution is used as the carrier medium to generate a thin jet that propagates at high flow rates22. In the second approach, viscous media such as grease or lipid/water mixtures, are used, which facilitates significantly slower flow rates27,32. The slow flow conserves protein. Here we demonstrate how phytochrome microcrystals can be used with both methods to obtain SFX crystal structures. We have developed two ways of producing phytochrome microcrystals, by crushing macrocrystals and by directly growing microcrystals in batch mode. This versatility allows upcoming SFX experiments on phytochromes to be optimised for hit rate, crystal quality and ease of crystal production. Although we have not verified the integrity of the crystals in grease spectroscopically, the SFX structures presented here confirm the structural integrity of the protein in both delivery methods.

It is currently unclear which conformational changes in the protein and the chromophore govern photo activation. To investigate this further, the microcrystals could be subjected to femtosecond time-resolved SFX. In the experiment, photo activated states are prepared by femtosecond optical laser pulses, which arrive at a defined delay time prior to the X-ray exposure. Time-resolved spectroscopy experiments of phytochromes33,34 indicate that high concentrations of the excited state can be prepared, but that the quantum yield of entering the photocycle upon return to the ground state is only on the order of 10-15%. Thus, it may be easiest to detect structural changes in the excited state, which lasts for a few hundreds of picoseconds. Observing conformational changes by crystallography also requires sufficient resolution. The resolution of 2.1 A, which we demonstrate here, should be fine enough to detect the isomerisation reaction in the chromophore, changes in water positions, and movement of amino acids side chains. The homogeneity of the crystal size and shape will be crucial since it affects laser penetration and excitation efficiency. Therefore the development of batch crystallisation for highly homogeneous microcrystals is important. The study presented here shows that the excellent diffraction data of the resting states of phytochromes can be recorded by SFX and encourages time-resolved SFX experiments.


Protein purification. Wild-type chromophore-binding domain (PAS-GAF) from Deinococcus radiodurans was expressed and purified following methods already described elsewhere10,35. Briefly, (His)6-tagged PAS-GAF was produced in E.coli strain BL21 DE3. The protein production was induced with IPTG, and biliverdin was added to the lysed cells to be incorporated overnight. The holoprotein was then purified with Ni-NTA affinity chromatography (HisTrap, GE healthcare) and size-exclusion chromatography (HiLoad 16/600 Superdex GE healthcare). The purified protein was concentrated to 25-30 mg/ml in buffer (30 mM Tris, pH 8.0) and was flash-frozen in liquid nitrogen.

Crystallisation. All crystals were set up and grown in complete darkness or under green safe light. Before crystallisation, the protein sample (PAS-GAF) was thawed and filtered with 0.2 |im centrifugal filter units. Vapour diffusion crystallisation was performed, where 20 mg/ml protein in buffer (30 mM Tris, pH 8.0) was mixed in a 1:1 ratio with reservoir solution using conditions previously described (67 mM Sodium acetate pH 4.95, 3.3%


PDB Code 5K5B 5L8M 5LBR

Data Collection

Collection Temperature 100 K 293 K 293 K

Space group C121 C121 C121

Cell dimensions

a, b, c (A) 93.76, 54.28, 70.15 94.10, 54.80, 69.90 96.22 55.49 71.63

a,|3,Y (°) 90.00, 92.20, 90.00 90.00, 92.60, 90.00 90.00 92.84 90.00

Resolution (A) 46.97-1.35 (1.38-1.35)* 38.20-2.10 (2.15-2.10) 71.55-2.20 (2.28-2.20)

Rmerge (%) 3.60 (56.0) — —

Rsplit (%) N/A 9.19 (85.43) 11.9 (67.7)

I/ctI 18.37 (2.31) 7.77 (1.80) 5.62 (0.68)

CC(1/2) 99.9 (76.5) 98.0 (49) 97.6 (60.4)

Completeness (%) 98.07 (95.69) 99.97 (99.8) 100(100)

Redundancy 4.36 (4.23) 819(526) 81.2 (24.1)

Number of hits N/A 114409 30146

Number of indexed hits N/A 90428 20014


Resolution (A) 39.37-1.35 (1.39-1.35) 38.20-2.10 (2.15-2.10) 71.55-2.20 (2.26-2.20)

Number of reflections 72086(5211) 18732 (1202) 18389(1326)

Rwork/Rfree 0.141/0.171 (0.260/0.288) 0.176/0.218 (0.299/0.297) 0.182/0.203 (0.496/0.483)

Number of atoms 2720 2505 2492

Average B factor (A2) 25.47 42.92 50.26

R.m.s. deviations

Bond lengths (A) 0.010 0.007 0.008

Bond angles (°) 1.506 1.313 1.287

Table 1. Data Collection and Refinement Statistics. tHighest resolution shell is shown in parenthesis.

PEG 400, 1 mM DTT)13, except for 30% 2-methyl-2, 4-pentanediol, which was included directly in the reservoir buffer. 2 |il hanging drops were equilibrated against 800 |il of reservoir solution. After 48 h incubation at room temperature, the formed crystals were flash-frozen in liquid nitrogen in cryoloops for crystallographic data collection.

For batch crystallisation of microcrystals, which was used in the LCLS experiment, 50 |il protein (25 mg/ml) were mixed with 450 |il of reservoir solution (60 mM Sodium acetate pH 4.95, 3.3% PEG 400, 1 mM DTT and 30% 2-methyl-2, 4-pentanediol) in 1.5 ml microcentrifuge tubes followed by immediate vortexing. The tubes were then constantly mixed on a rocking table at 12 °C for ~36 h, yielding homogenous mixture of microcrystals of 10-20 |im size. The crystals were concentrated by centrifugation into a crystal concentration up to 20% to increase crystal hit rate and filtered directly before injection to minimize clogging of the jet.

For preparation of microcrystals by crushing macrocrystals, which were used at SACLA, the above vapour diffusion crystallisation conditions were used, but the macrocrystals were grown in sitting drops with 40 |il total volume. The crystal-containing drops were pooled in a microcentrifuge tube and the crystals were crushed by vortexing with seed beads (Molecular dimensions) for 30 s, after which the tubes were cooled on ice. The size of the crystals was estimated under the microscope to be ~10-20 |im, and oversized crystals were excluded with a 20 |im cutoff centrifugal filter (Partec). Crystals were concentrated and mixed with a grease (Superlube, Syncho chemical corp.) prior to loading into the syringe pump injector as described elsewhere27.

Data collection and processing at the ESRF. The low temperature (LowT) macrocrystal data were acquired at beamline ID23-1 of the European Synchrotron Radiation Facility (ESRF), in a 100 K cryostream and with an X-ray wavelength of 0.980 A. Diffraction data were processed using the XDS program package version January 10, 201436. The LowT crystal belong to monoclinic space group C121 with one monomer in an asymmetric unit. The diffraction data were cut at a cross-correlation (CC1/2) value of 0.7637, which corresponds the high resolution limit of 1.35 A. The statistics of data collection, processing, structure determination and refinement of all data sets are summarized in Table 1.

Data collection and processing at the LCLS. SFX data of microcrystals were collected at the coherent X-ray Imaging CXI beamline at Linac Coherent Light Source (LCLS) using 1.8 mJ/pulse at 9.5 keV X-rays with a pulse duration of ~35 fs and a repetition rate of 120 Hz23. After concentrating and filtering through a 20 |im cutoff filter, the crystal suspensions were injected into the X-ray beam with gas virtual dynamic nozzles ranging from 50 |im to 100 |im diameter22, using a pressurized reservoir mounted on an antisettling device (available at LCLS). The flow rates of the liquid gas and shielding gas were adjusted to obtain a stable jet with a flow rate averaging around 30 |il/min. The time between filtering and injection was kept minimal to avoid excessive clogging of the reservoir and tubing.

The diffraction images were recorded with Cornell-SLAC Pixel Array detectors, consisting of 64 tiles (each 194 pixels by 185 pixels) with a pixel size of 110 x 110 |im2. A crystal "hit" was defined as an image containing a minimum of 20 diffraction peaks. Crystal hits were identified using the software package Cheetah24. The crystal hit rate was typically 2-5% and appeared to be limited by the tendency of the crystals to aggregate, which blocked the tubing or reservoir. The collected data were processed using Cheetah24 and CrystFEL25. The indexing rate was stable at around 70-80%. The crystals belonged to C121 space group with highly similar cell parameters to LowT crystals (Table 1).

SFX data collection and processing at SACLA. SFX diffraction data were collected at the BL3 beamline at SACLA38 using 7.0 keV X-rays with a pulse duration of <10 fs and a repetition rate of 30 Hz. The syringe-pump injector was installed in the diffraction chamber filled with helium39. Phytochrome crystals were dispersed in grease27 and extruded through 110 |im diameter nozzle at a flow rate of 500 nl per minute. Diffraction patterns were recorded on a multiport CCD detector with an eight sensor module40. The crystal hit rate varied between 5 and 15%. The raw images were filtered and converted by Cheetah pipeline adapted for SACLA41 and the processing was performed as described above. For further details on number of indexed patterns etc., see Table 1.

Structure determination and refinement. The structures were solved by molecular replacement with Phaser42 using the high-resolution structure of D. radiodurans PAS-GAF fragment 4Q0H9 as a search model. The models were built in Coot43, and structural refinement was performed with REFMAC5 version 5.8.013544. In all refinements, the same topology/parameter (CIF) file for the chromophore was used as in ref. 9. In the LowT structure refinement, the X-ray matrix-weighing term of 0.5 was used. The structure was refined with anisotropic temperature factors, resulting final Rwork/Rfree of 14.2/17.0%. Ramachandran statistics of the final LowT structure had 97.8% in preferred and 2.2% in allowed regions with 0% outliers. In SFX structures (LCLS and SACLA) refinement, the weighing term of 0.05 was used. All structure figures were generated using PyMOL (DeLano Scientific, San Carlos, California, USA). All structure have been deposited to Protein Data Bank.


1. Bae, G. & Choi, G. Decoding of light signals by plant phytochromes and their interacting proteins. Annu. Rev. Plant Biol. 59, 281-311 (2008).

2. Hughes, J. Phytochrome cytoplasmic signaling. Annu. Rev. Plant Biol. 64, 377-402 (2013).

3. Gan, F. et al. Extensive remodeling of a cyanobacterial photosynthetic apparatus in far-red light. Science 345, 1312-1317 (2014).

4. Davis, S. J., Vener, A. V. & Vierstra, R. D. Bacteriophytochromes: Phytochrome-like photoreceptors from nonphotosynthetic eubacteria. Science 286, 2517-2520 (1999).

5. Auldridge, M. E. & Forest, K. T. Bacterial phytochromes: More than meets the light. Crit. Rev. Biochem. Mol. Biol. 46, 67-88 (2011).

6. Yang, X., Kuk, J. & Moffat, K. Crystal structure of Pseudomonas aeruginosa bacteriophytochrome: Photoconversion and signal transduction. Proc. Natl. Acad. Sci. USA 105, 14715-14720 (2008).

7. Essen, L. O., Mailliet, J. & Hughes, J. The structure of a complete phytochrome sensory module in the Pr ground state. Proc. Natl. Acad. Sci. USA 105, 14709-14714 (2008).

8. Wagner, J. R., Zhang, J. R., Brunzelle, J. S., Vierstra, R. D. & Forest, K. T. High resolution structure of Deinococcus bacteriophytochrome yields new insights into phytochrome architecture and evolution. J. Biol. Chem. 282, 12298-12309 (2007).

9. Burgie, E. S. et al. Crystallographic and electron microscopic analyses of a bacterial phytochrome reveal local and global rearrangements during photoconversion. J. Biol. Chem. 289, 24573-24587 (2014).

10. Takala, H. et al. Signal amplification and transduction in phytochrome photosensors. Nature 509, 245-248 (2014).

11. Stojkovic, E. A. et al. FTIR Spectroscopy Revealing Light-Dependent Refolding of the Conserved Tongue Region of Bacteriophytochrome. J. Phys. Chem. Lett. 5, 2512-2515 (2014).

12. Song, C. et al. Two ground state isoforms and a chromophore D-ring photoflip triggering extensive intramolecular changes in a canonical phytochrome. Proc. Natl. Acad. Sci. USA 108, 3842-3847 (2011).

13. Wagner, J. R., Brunzelle, J. S., Forest, K. T. & Vierstra, R. D. A light-sensing knot revealed by the structure of the chromophore-binding domain of phytochrome. Nature 438, 325-331 (2005).

14. Burgie, E. S., Zhang, J. & Vierstra, R. D. Crystal Structure of Deinococcus Phytochrome in the Photoactivated State Reveals a Cascade of Structural Rearrangements during Photoconversion. Structure 24, 448-457 (2016).

15. Tenboer, J. et al. Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein. Science 346, 1242-1246 (2014).

16. Chapman, H. N. et al. Femtosecond X-ray protein nanocrystallography. Nature 470, 73-78 (2011).

17. Kirian, R. A. et al. Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals. Acta Crystallogr. A. 67, 131-140 (2011).

18. Barends, T. R. M. et al. Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation. Science 350, 445-450 (2015).

19. Neutze, R., Wouts, R., van der Spoel, D., Weckert, E. & Hajdu, J. Potential for biomolecular imaging with femtosecond X-ray pulses. Nature 406, 752-757 (2000).

20. Hirata, K. et al. Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL. Nat. Methods 11, 734-736 (2014).

21. Bhattacharya, S., Auldridge, M. E., Lehtivuori, H., Ihalainen, J. A. & Forest, K. T. Origins of fluorescence in evolved bacteriophytochromes. J. Biol. Chem. 289, 32144-32152 (2014).

22. DePonte, D. P. et al. Gas dynamic virtual nozzle for generation of microscopic droplet streams. J. Phys. D. Appl. Phys. 41, 195505 (2008).

23. Boutet, S. & J Williams, G. The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). New J. Phys. 12, 035024 (2010).

24. Barty, A. et al. Cheetah: software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data. J. Appl. Crystallogr. 47, 1118-1131 (2014).

25. White, T. A. et al. CrystFEL: a software suite for snapshot serial crystallography. J. Appl. Crystallogr. 45, 335-341 (2012).

26. Fraser, J. S. et al. Accessing protein conformational ensembles using room-temperature X-ray crystallography. Proc. Natl. Acad. Sci. USA 108, 16247-16252 (2011).

27. Sugahara, M. et al. Grease matrix as a versatile carrier of proteins for serial crystallography. Nat. Methods 12, 61-63 (2015).

28. Auldridge, M. E., Satyshur, K. A., Anstrom, D. M. & Forest, K. T. Structure-guided engineering enhances a phytochrome-based infrared fluorescent protein. J. Biol. Chem. 287, 7000-7009 (2012).

29. Burgie, E. S., Bussell, A. N., Walker, J. M., Dubiel, K. & Vierstra, R. D. Crystal structure of the photosensing module from a red/far-red light-absorbing plant phytochrome. Proc. Natl. Acad. Sci. USA 111, 10179-10184 (2014).

30. Li, F. et al. X-ray radiation induces deprotonation of the bilin chromophore in crystalline D. radiodurans phytochrome. J. Am. Chem. Soc. 137, 2792-2795 (2015).

31. Kim, P. W., Rockwell, N. C., Martin, S. S., Lagarias, J. C. & Larsen, D. S. Dynamic inhomogeneity in the photodynamics of cyanobacterial phytochrome Cph1. Biochemistry 53, 2818-2826 (2014).

32. Weierstall, U. et al. Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography. Nat. Commun. 5, 3309 (2014).

33. Dasgupta, J., Frontiera, R. R., Taylor, K. C., Lagarias, J. C. & Mathies, R. A. Ultrafast excited-state isomerization in phytochrome revealed by femtosecond stimulated Raman spectroscopy. Proc. Natl. Acad. Sci. USA 106, 1784-1789 (2009).

34. Toh, K. C. et al. Primary Reactions of Bacteriophytochrome Observed with Ultrafast Mid-Infrared Spectroscopy. J. Phys. Chem. A 115, 3778-3786 (2011).

35. Lehtivuori, H. et al. Fluorescence properties of the chromophore-binding domain of bacteriophytochrome from Deinococcus radiodurans. J Phys Chem B 117, 11049-11057 (2013).

36. Kabsch, W. Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants. J. Appl. Crystallogr. 26, 795-800 (1993).

37. Karplus, P. A. & Diederichs, K. Linking Crystallographic Model and Data Quality. Science 336, 1030-1033 (2012).

38. Tono, K. et al. Beamline, experimental stations and photon beam diagnostics for the hard x-ray free electron laser of SACLA. New J. Phys. 15, 083035 (2013).

39. Tono, K. et al. Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser. J. Synchrotron Radiat. 22, 532-537 (2015).

40. Kameshima, T. et al. Development of an X-ray pixel detector with multi-port charge-coupled device for X-ray free-electron laser experiments. Rev. Sci. Instrum. 85, 033110 (2014).

41. Nakane, T. et al. Data processing pipeline for serial femtosecond crystallography at SACLA. J. Appl. Crystallogr. 49, 1035-1041 (2016).

42. Storoni, L. C., McCoy, A. J. & Read, R. J. Likelihood-enhanced fast rotation functions. Acta Crystallogr. D 60, 432-438 (2004).

43. Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D 60, 2126-2132 (2004).

44. Murshudov, G. N. et al. REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr. D 67, 355-367 (2011).


SW acknowledges funding from the European Research Council and the Foundation of Strategic Research, Sweden. EAS acknowledges funding from National Science Foundation (NSF) MCB grant 1413360. JAI, HT and HL acknowledge funding from Academy of Finland and Finnish Cultural Foundation and the support in protein production provided by Tampere Protein Technologies Core Facility (V. Hytönen and J. Määttä) under Biocenter Finland network. SW, GG, and JI acknowledge support by the Foundation of International Cooperation in Research and Higher Education, Sweden (STINT). EN, TN, and TT acknowledge support by X-ray Free Electron Laser Priority Strategy Program (MEXT, Japan) and computing supports from SACLA HPC and Mini-K. Part of the experiments were carried out at the BL3 of SACLA with the approval of the Japan Synchrotron Radiation Research Institute (JASRI) (proposal no. 2014B8050). JCHS, PF and MS acknowledge NSF STC 1231306. Portions of this research were carried out at the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory. Use of the LCLS, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515 Parts of the sample delivery system used at LCLS for this research was funded by the NIH grant P41GM103393, formerly P41RR001209. TW, AB, and KP acknowledge financial support from CFEL DESY through program-oriented funds of the Helmholtz Association.

Author Contributions

S.W., H.T. and P.E. designed the experiments, all authors performed the experiments, H.T., E.C. P.E., K.P., R.D., A.B., T.W., S.P., I.P. and M.S. analysed the data, E.S., E.C., P.E., H.T. and L.H. prepared the protein crystals, and S.W., E.C., H.T., P.E., M.S. and E.S. wrote the paper with input from all authors.

Additional Information

Competing financial interests: The authors declare no competing financial interests.

How to cite this article: Edlund, P. et al. The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography. Sci. Rep. 6, 35279; doi: 10.1038/srep35279 (2016).

l/gv (J) I This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit

© The Author(s) 2016