Scholarly article on topic 'COL4A1 Mutations Cause Ocular Dysgenesis, Neuronal Localization Defects, and Myopathy in Mice and Walker-Warburg Syndrome in Humans'

COL4A1 Mutations Cause Ocular Dysgenesis, Neuronal Localization Defects, and Myopathy in Mice and Walker-Warburg Syndrome in Humans Academic research paper on "Biological sciences"

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Academic research paper on topic "COL4A1 Mutations Cause Ocular Dysgenesis, Neuronal Localization Defects, and Myopathy in Mice and Walker-Warburg Syndrome in Humans"

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COL4A1 Mutations Cause Ocular Dysgenesis, Neuronal Localization Defects, and Myopathy in Mice and WalkerWarburg Syndrome in Humans

Cassandre Labelle-Dumais1. David J. Dilworth1®", Emily P. Harrington1, Michelle de Leau1, David Lyons1, Zhyldyz Kabaeva2, M. Chiara Manzini3, William B. Dobyns4, Christopher A. Walsh3, Daniel E. Michele2, Douglas B. Gould1*

1 Departments of Ophthalmology and Anatomy, Institute for Human Genetics, University of California San Francisco School of Medicine, San Francisco, California, United States of America, 2 Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, United States of America, 3 Division of Genetics and The Manton Center for Orphan Disease Research, Children's Hospital Boston, Howard Hughes Medical Institute, and Harvard Medical School, Boston, Massachusetts, United States of America, 4 Departments of Human Genetics, Neurology, and Pediatrics, University of Chicago, Chicago, Illinois, United States of America


Muscle-eye-brain disease (MEB) and Walker Warburg Syndrome (WWS) belong to a spectrum of autosomal recessive diseases characterized by ocular dysgenesis, neuronal migration defects, and congenital muscular dystrophy. Until now, the pathophysiology of MEB/WWS has been attributed to alteration in dystroglycan post-translational modification. Here, we provide evidence that mutations in a gene coding for a major basement membrane protein, collagen IV alpha 1 (COL4A1), are a novel cause of MEB/WWS. Using a combination of histological, molecular, and biochemical approaches, we show that heterozygous Col4a1 mutant mice have ocular dysgenesis, neuronal localization defects, and myopathy characteristic of MEB/WWS. Importantly, we identified putative heterozygous mutations in COL4A1 in two MEB/WWS patients. Both mutations occur within conserved amino acids of the triple-helix-forming domain of the protein, and at least one mutation interferes with secretion of the mutant proteins, resulting instead in intracellular accumulation. Expression and posttranslational modification of dystroglycan is unaltered in Col4a1 mutant mice indicating that COL4A1 mutations represent a distinct pathogenic mechanism underlying MEB/WWS. These findings implicate a novel gene and a novel mechanism in the etiology of MEB/WWS and expand the clinical spectrum of COL4A1 -associated disorders.

Citation: Labelle-Dumais C, Dilworth DJ, Harrington EP, de Leau M, Lyons D, et al. (2011) COL4A1 Mutations Cause Ocular Dysgenesis, Neuronal Localization Defects, and Myopathy in Mice and Walker-Warburg Syndrome in Humans. PLoS Genet 7(5): e1002062. doi:10.1371/journal.pgen.1002062

Editor: Nancy B. Spinner, University of Pennsylvania, United States of America

Received October 8, 2010; Accepted March 18, 2011; Published May 19, 2011

Copyright: © 2011 Labelle-Dumais et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: This work was supported in part by That Man May See, The Sandler Family Foundation, Research to Prevent Blindness, Muscular Dystrophy Association (all to DBG), an Unrestricted Grant from Research to Prevent Blindness, and an NEI core Grant (EY02162) (UCSF Department of Ophthalmology). DJD was supported by the Canadian Stroke Network. MCM was supported by a Development Grant from the Muscular Dystrophy Association. MdL is supported by a fellowship from de Hersenstichting Nederland. CAW is an investigator of the Howard Hughes Medical Institute and also acknowledges NINDS R01 NS032457. WBD acknowledges NIH grants 1P01-NS039404 and 1R01-NS050375 and the Brain and Tissue Banks for Developmental Disorders, NIH contract number N01-HD-3-3199. DEM acknowledges NHLBI grant HL080388. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests: The authors have declared that no competing interests exist. * E-mail: . These authors contributed equally to this work.

n Current address: Institute for Systems Biology, Seattle, Washington, United States of America


Congenital muscular dystrophies (CMDs) involving ocular and cerebral malformations are devastating childhood diseases. Fukuyama congenital muscular dystrophy, Muscle-Eye-Brain disease (MEB), and Walker-Warburg Syndrome (WWS) are clinically and mechanistically related forms of CMD [1-4]. Patients present at birth or as infants with muscle weakness, hypotonia or even severe myopathy leading to fatal respiratory insufficiency. Clinical presentation varies between patients but often includes myofiber necrosis and fibrosis, replacement by adipose tissue, split muscle fibers and the presence of non-peripheral nuclei. In addition, while most patients exhibit marked elevation of serum creatine kinase (CK), others have CK levels within the normal range [5].

Ocular and cerebral phenotypes demonstrate variable penetrance and expressivity between individual patients. For instance, ocular dysgenesis can occur in either, or both, the anterior (Peters' anomaly, Rieger syndrome, cataracts, buphthalmos and developmental glaucoma) and posterior portions of the eye (retinal dysplasia, retinal detachments and optic nerve hypoplasia) [6-8]. Variable neurological manifestations, including mental retardation and epilepsy, may be at least partially explained by cerebral cortical malformations including cobblestone lissencephaly, cere-bellar hypoplasia, hydrocephalus and encephalocele.

Genetic and biochemical studies led to the identification of a number of genes involved in the etiology of CMDs and revealed that alterations in post-translational processing of dystroglycan underlie MEB/WWS [9-15]. In these 'dystroglycanopathies', hypoglycosylation of dystroglycan disrupts ligand binding and

Author Summary

Muscle-eye-brain disease (MEB) and Walker-Warburg Syndrome (WWS) are devastating childhood diseases that belong to a subgroup of congenital muscular dystrophies (CMDs) characterized by ocular dysgenesis, neuronal migration defects, and congenital myopathy. Genetic studies have revealed a number of genes involved in the etiology of CMDs, and subsequent studies show that alterations in dystroglycan glycosylation underlie MEB/ WWS. However, over half of MEB/WWS patients do not have mutations in known genes encoding glycosyltrans-ferases, suggesting that other genes are involved. Here, we describe a novel and genetically complex mouse model for MEB/WWS and identify putative heterozygous mutations in COL4A1 in two MEB/WWS patients. We identify a novel gene implicated in the etiology of MEB/WWS, provide evidence of mechanistic heterogeneity for this subgroup of congenital muscular dystrophies, and develop an assay to test the functional significance of putative COL4A1 mutations. Our findings represent the first evidence for a dominant mutation leading to MEB/WWS-like diseases and expand the spectrum of clinical disorders resulting from Col4a1/COL4A1 mutations.

impairs muscle fiber attachment to the extracellular matrix. Central nervous system pathology is proposed to be secondary to defective interactions between radial glial cells and the pial basement membrane [16-18]. Despite these major advances, over half of MEB/WWS patients do not have mutations in known genes encoding glycosyltransferases [19,20], suggesting that other genes in this pathway contribute to disease or that independent mechanisms are responsible. Identifying new pathways involved in MEB/WWS will be an important breakthrough that could open new avenues for understanding and ultimately treating CMDs.

We have recently discovered the first mutations in the gene coding for the ubiquitous basement membrane protein type IV collagen alpha 1 in mice (Col4a1) and humans (COL4A1) [21]. COL4A1 is the most abundant basement membrane protein and is ubiquitously present in basement membranes with few exceptions. The collagenous domain (a long stretch of Gly-Xaa-Yaa repeats that forms a triple helix) accounts for over 90% of the protein. Mutations in this triple helix-forming domain are well documented to be pathogenic in several types of collagens, including type IV collagens. The mutation we identified in mice (referred to as Col4a1Aex40) disrupts a splice acceptor site, causing exon 40 to be skipped and concomitant deletion of 17 amino acids from the collagen triple helical domain which interferes with proper folding, assembly and secretion of heterotrimeric COL4A1 and COL4A2 [21,22]. To date, eleven out of twelve other Col4a1 mutations reported in mice [23,24] and seventeen out of twenty one COL4A1 mutations identified in humans [21,22,25-34] are missense mutations within the triple helical domain, demonstrating that alterations of this domain are highly pathogenic.

In mice and humans, semi-dominant Col4a1/COL4A1 mutations are highly pleiotropic with variable expressivity. The tissue distribution and severity of pathology depends on genetic and environmental factors but commonly include cerebrovascular diseases, ocular and renal defects [21-24,35]. Here, we broaden this spectrum to include MEB/WWS. We show that, depending on the genetic context; Col4a1+/A'x40 mice recapitulate the pathophysiological hallmarks of MEB/WWS, including ocular

anterior segment dysgenesis, optic nerve hypoplasia, cortical lamination defects and myopathy. In addition, we identify heterozygous mutations in the triple-helix-forming domain of COL4A1 in two MEB/WWS patients. Together, these findings support COL4A1 mutations as a novel genetic cause of MEB/ WWS. Importantly, COL4A1 is not directly related to post-translational modification of dystroglycan. We show that the mechanism is independent of dystroglycan glycosylation and instead is probably due to decreased COL4A1 levels in basement membranes. COL4A1 mutations are pleiotropic and these data describe another clinically distinct group of diseases that can result from alterations in a single gene.


Mislocalization and increased apoptosis of retinal ganglion cells cause optic nerve hypoplasia

Ocular hallmarks of MEB/WWS include anterior segment dysgenesis and optic nerve hypoplasia. Depending on the genetic context, mutations in Col4a1 can cause both anterior segment dysgenesis and optic nerve hypoplasia although the underlying pathogenic mechanism(s) remain unexplored [23,24,35]. During development, COL4A1 is present in the inner limiting membrane of the retina [36] and inner limiting membrane disruptions can perturb retinal ganglion cell (RGC) localization and cause apoptotic death [37]. To determine if excess RGC death during development caused optic nerve hypoplasia, developing retinas were immunolabeled for Islet-1 (ISL1) and Laminin, which mark newly specified RGCs [38] and basement membranes, respectively. At embryonic days (E) 14, E16 and E18, RGCs were located in the innermost part of the retina in Col4a1+/+ mice, with only occasional ISL1 immunoreactivity detected in the outer retina (Figure 1D). In contrast, in Col4a1+/A'x40 mice, the thickness of the ISL1 positive layer was highly variable and there were more displaced ISL1 positive cells detected in the outer retina (arrows in Figure 1H and 1L). Laminin immunoreactivity revealed focal disruptions in the inner limiting membranes of Col4a1+/A'x40 animals (asterisks in Figure 1F) that were not observed in Col4a1+/+ animals. Moreover, in contrast to Col4a1+/+ eyes where the vasculature is closely associated with the inner limiting membrane, the hyaloid vasculature in Col4a1+/A'x40 eyes is most often found in the vitreous (Figure 1B and 1F) and, in one extreme case, the posterior chamber was devoid of detectable vasculature (Figure 1J).

During normal retinal development, approximately 50% of RGCs undergo programmed cell death as the visual system matures [39,40]. Induced or genetic disruption of the inner limiting membrane perturbs RGC localization and leads to RGC apoptosis during embryogenesis [37]. Therefore, we hypothesized that Col4a1+/A'x40 mice might exhibit increased RCG apoptosis. To test this, we co-labeled retinal sections with antibodies against ISL1 and activated Caspase-3 (Figure 2A-2F) and calculated the apoptotic index by counting the number of ISL1/Caspase 3 double-labeled cells (Figure 2G). As retinas from Col4a1+/A'x40 mice were smaller than retinas from Col4a1+/+ mice (Figure 2H), we normalized the number of double-labeled cells to the retinal cross-sectional area. Col4a1+/+ mice had low levels of ganglion cell apoptosis at E14 and E16 that increased approximately 2-fold by E18. Although apoptotic rates in Col4a1+/A'x40 mice at E14 and E16 were not significantly different from those observed in Col4a1+/+ mice, there was a significant increase in apoptosis of ISL1 positive cells in Col4a1+/A'x40 eyes compared to Col4a1+/+ eyes at E18 (p<0.05) - especially among mislocalized ISL1 labeled cells (Figure 2F). Of note, one E18 Col4a1+/A'x40 eye had an

A lslet-1 Col4a1+/+ B Laminin '„ » C DAPI D y

E Col4a1+Mex40 F » a * G H

Col4a 1 +/Aex40 J K L

Figure 1. Mislocalization of retinal ganglion cells in Col4a1+/Dex40 eyes. Representative position-matched transverse sections from Col4a1+/+ (A-D) and Col4a1+/Aex40 (E-L) eyes at E18 labeled with anti-ISLI (green in A, E, and I), anti-laminin (red in B, F and J) and DAPI (blue in C, G, and K). Anti-ISLI labeling revealed an increased number of RGCs in the outer retinas (arrows in D, H and L) of the mutant eyes. Labeling with anti-laminin antibody revealed focal disruptions in the inner limiting membrane in Col4a1+/Aex40 mice (asterisks in F) and demonstrates that the retinal vasculature is adjacent to the inner limiting membrane in Col4a1+/+ eyes but not in Col4a1+/Aex40 eyes (arrowheads in B and F). Notably, in one extreme case, we detected no hyaloid vasculature and markedly reduced retinal thickness in the eye shown in I-L. Scale bar: 50 mm. doi:10.1371/journal.pgen.1002062.g001

extremely high apoptotic index (12.9) that was considered an vasculature in the posterior chamber of this eye suggesting that outlier and was removed from subsequent statistical analyses (see retinal vasculature might directly contribute to the inner limiting Materials and Methods). Interestingly, we did not observe membrane or affect ganglion cell viability in other ways. Together,

A lslet-1 B Activated Csp3 C

Col4a1+/+ ■ ■ ^

Figure 2. Increased apoptosis of Islet-1 positive retinal ganglion cells in Col4a1+/Dex40eyes. Representative eye sections from E18 Col4a1+/+ mice (A-C) and Col4a1+'Aex40 mice (D-F) co-labeled with anti-ISL1 (green in A and D) and anti-activated Caspase-3 (red in B and E). Co-labeling for ISL1 and Caspase-3 revealed an increase in the number of apoptotic retinal ganglion cells in Col4a1+/Aex40 eyes. Col4a1+/Aex40 eyes were significantly smaller than Col4a1+/+ eyes at all ages examined (H; E14 p = 0.01; E16 p = 0.04; E18 p<10—5 comparing at least 6 eyes for Col4a1+/+ (blue) and Col4a1+/Aex40 (red) at each age). After correcting for size, there was a significant increase in the number of apoptotic retinal ganglion cells in Col4a1+/Aex40 eyes at E18 (G; p = 0.02 comparing at least 6 eyes for Col4a1+/+ (blue) and Col4a1+/Aex40 (red) at each age). In G and H, data are presented as mean +/— SEM. Scale bar: 50 mm. doi:10.1371/journal.pgen.1002062.g002

our data support that Col4a1 mutation leads to focal disruptions of the inner limiting membrane and that optic nerve hypoplasia results both from reduced production of retinal neurons and from mislocalization and subsequent apoptosis of ganglion cells during development.

Col4a1+/Aex40 mice display cerebral cortical malformations and cerebral neuronal localization defects characteristic of cobblestone lissencephaly

Based on our observations in the retina, we predicted that Col4a1+/Aex40 mice might also show pial basement membrane disruptions and cerebral cortical lamination defects that model cobblestone lissencephaly seen in MEB/WWS. To test our hypothesis, we stained coronal brain sections from adult Col4a1+/+ and Col4a1+/A'x40 mice with cresyl violet and detected abnormalities in all of the Col4a1+/Aex40 but none of the Col4a1+/+ mice examined (Figure 3). All Col4a1+/A'x40 mice had focal and variable cerebral cortex lamination defects ranging from mild distortions and ectopias to severe heterotopic regions devoid of obvious lamination (Figure 3B-3F). Occasionally, Col4a1+/A'x40 mice displayed enlarged ventricles or major structural abnormalities (Figure 3G and 3H). Immunolabeling with the pan-neuronal marker NeuN confirmed the neuronal identity of the ectopic cells (Figure 4). Col4a1+/A'x40 mice also had subtle but consistent defects within the hippocampus (Figure S1). The CA1, CA3 and dentate gyrus layers of Col4a1+/A'x40 mice were less tightly organized and generally more dispersed compared to Col4a1+/+ mice and local perturbations were common. As it is the case in other animal models of MEB/WWS [41,42], enhanced glial fibrillary acid protein (GFAP) immunoreactivity, which is reflective of astrocytic gliosis, was observed in the hippocampus and cerebral cortex of Col4a1+/A'x40 mice (Figure S2).

Neuronal localization defects in Col4a1+/Aex40 mice are congenital

To determine if cortical malformations were congenital or acquired, we used bromodeoxyuridine (BrdU) pulse labeling in utero to evaluate the localization of neurons that underwent terminal cell division during defined stages of embryogenesis. The locations of cells labeled at E14 or E16 were determined at birth (P0). In all Col4a1+/+ mice, BrdU-labeled neurons were uniformly distributed primarily in the superficial cortex (Figure 5A and 5F). In contrast, the distribution of BrdU-labeled cells in mutant animals demonstrated that cortical lamination was disorganized. Focal and variable lamination defects were completely penetrant in Col4a1+/Aex40 mice (Figure 5B-5E and 5G-5J). Laminin labeling of basement membranes in P0 mice revealed discontinuous pial basement membranes in all mutant mice, notably in areas adjacent to ectopias (Figure 6). Together, these findings demonstrate that Col4a1+/A'x40 mice have abnormal neuronal localization typical of cobblestone lissencephaly observed in MEB/WWS patients and suggest that these congenital defects are secondary to breaches in the pial basement membrane.

Genetically modifiable myopathy in Col4a1+/Aex40 mice

Because Col4a1+/'x40 mice display ocular and cerebral abnormalities characteristic of MEB/WWS, we hypothesized that they would also have myopathy. To test this, we first confirmed that COL4A1 was present in skeletal muscle basement membrane by immunolabeling (Figure S3) and performed functional, biochemical and histological analyses of muscles from young and aged Col4a1+/+ and Col4a1+/A'x40 mice. At 3 months of age, Col4a1+/A'x40 mice performed significantly worse than controls in a

Figure 3. Coi4a1+De''40 mice have focal and variable cortical malformations. Representative cresyl violet stained coronal brain sections from adult Col4a1+/+ (A) and Col4a1+/Aex40 (B-H) revealed cortical malformations characteristic of cobblestone lissencephaly in all mutant brains examined (n = 6). In contrast to the well-defined cortical lamination observed in Col4a1+/+ brains, Col4a1+/Aex40 brains had variable abnormalities including disorganized cortical lamination (bracket in B), ectopias (C, enlarged in D), heterotopic regions (E and F), enlarged ventricles (G) and, occasionally, severe cortical malformations (H). Scale bars: A-C and E-H, 800 mm; d, 200 mm. doi:10.1371/journal.pgen.1002062.g003

test of peak grip force (Figure 7A). Next, we analyzed serum CK activity before and after exercise. We found no significant difference in CK levels between control and mutant mice at baseline, however, Col4a1+/A'x40 mice had a significant elevation in CK activity following exercise compared to pre-exercise Col4a1+/Aex4° and post-exercise Col4a1+/+ mice (Figure 7B). Consistent with these functional and biochemical data, we also detected histological differences between Col4a1+/+ and Col4a1+/A'x40 muscles. Compared to Col4a1+/+ littermates, Col4a1+/A'x40 mice had occasional split muscle fibers and a significant increase in the number of non-peripheral nuclei - a measure of myopathy (Figure 7C-7E). Importantly, the severity of myopathy was not markedly affected by age.

We have shown previously that ocular dysgenesis is genetic context-dependent and that mutant F1 progeny of C57BL/6J and CAST/EJ crosses (CASTB6F1) are morphologically rescued [35]. As shown in Figure 7C, in contrast to what is observed on the C57BL/6J background, the number of non-peripheral nuclei was not increased in CASTB6F1 Col4a1+/A'x40 mice compared to their

Figure 4. Col4a1+/Dex40 mice display cortical neuronal localization defects. Representative images of NeuN-labeled coronal brain sections from adult Col4a1(/( (A and C) and Col4a1+/Jex40 (B and D-F) mice revealed heterotopic regions (asterisks in B and E) and ectopias (arrows in B and D) in all mutant brains examined. NeuN labeling of ectopic cells located in the marginal zone (F, enlarged from box in E) confirmed their neuronal identity. Scale bars: A-E, 500 mm; F, 125 mm. doi:10.1371/journal.pgen.1002062.g004

Col4a1+/+ littermates. Moreover, there were significantly fewer muscle fibers with non-peripheral nuclei in CASTB6F1 Col4a1+/A'x40 mice compared to C57BL/6J Col4a1+/A'x40 mice (p<0.05), indicating that the CAST/EJ strain has one or more loci that can also ameliorate Col4a1-induced myopathy.

Identification of COL4A1 mutations in human MEB/WWS patients

Col4a1+/Aex40 mice display multiple hallmarks of MEB/WWS raising the possibility that COL4A1 mutations might cause CMD-like diseases in human patients. To test this, we performed direct sequence analysis of genomic DNA from a cohort of 27 patients with CMD (see Table S1 for primers). Fifteen patients had diagnoses of WWS, two had diagnoses of MEB, and nine were not specifically classified as either but had CMD with variable ocular and cerebral involvement. To enrich for patients that may have dominant or semi-dominant mutations, rather than recessive mutations, most of the patients (23 out of 27) were chosen from non-consanguineous families. Finally, all patients were negative for mutations in genes currently known to underlie MEB/WWS-like diseases including LARGE, POMT1, POMT2, POMGNT1, FKTN and FKRP.

We identified several coding and non-coding sequence variants in COL4A1 (Tables S2, S3, S4). Twelve coding variants were silent and were either observed in controls or were not predicted to be splice-site-altering variants [43]. We identified four non-synonymous coding variants. Two of the four non-synonymous variants were previously identified SNPs that were highly polymorphic in patients and in controls and therefore deemed unlikely to be

Figure 5. Focal and variable cortical neuronal localization defects are developmental. Embryos pulsed-labeled with BrdU at E14 were harvested at birth and BrdU was immunolabeled in position-matched coronal brain sections of Col4a1(/( (A and F) and Col4a1+/Dex40 mice (B-E and G-J). In contrast to Col4a1+/+ controls, in which BrdU-labeled cells are distributed in a uniform layer in the outer cortex, all Col4a1+/Aex40 brains had focal neuronal localization defects that ranged in severity from diffuse cell layers to ectopias and severely disorganized cortical lamination (note gradient of severity from B through E and from G through J). Similar results were observed in embryos labeled at E16. Scale bar: 500 mm.


pathogenic. The two remaining SNPs were rare, missense variants that have not been previously reported in dbSNP and were heterozygous in independent patients. The first mutation was identified in a patient diagnosed with WWS that had lissenceph-aly, hydrocephalus, Dandy-Walker malformation, optic nerve hypoplasia and was hypotonic with CMD (see Figure S4 and Text S1 for clinical details). An adenine to guanine transition (A3046G) in exon 36 substituted a methionine residue for a valine residue



Figure 6. Col4a1+/Jex40 mice have focal breaches of the pial basement membrane during development. Representative images of laminin labeled (green) coronal brain sections from P0 Col4a1+/+ (A-D) and Col4a1+/Aex40 (E-L) revealed breaches of the pial basement membrane in mutant animals. All sections of Col4a1+/+ brains examined (A-D) showed intact pial membranes (higher magnifications in D). In contrast, position-matched Col4a1+/Aex40 brains display multiple focal disruptions of the pial basement membrane (arrows and asterisks in G and K) that are shown in higher magnification in H, and L. Notably, the high magnification image in L shows ectopic cells (DAPI in blue) that have breached the pial membrane adjacent to a disruption. Scale bars: A-C, E-G, and I-K, 500 mm; D, H and L, 50 mm. doi:10.1371/journal.pgen.1002062.g006

within the triple helical domain at amino acid number 1016 (p.M1016V) and was not detected in 282 control chromosomes (Figure 8A).

The second mutation was identified in a patient with unspecified CMD with ocular and cerebral involvement. The patient had mild gyral abnormalities, hydrocephalus, retinal dysplasia, seizures and elevated CK (see Figure S4 and Text S1 for clinical details). A cytosine to guanine transversion (C3946G) in exon 44 substituted a glutamine residue for a glutamate residue within the triple helical domain of the protein at amino acid number 1316 (p.Q1316E) (Figure 8A). Although this cytosine to guanine transversion was not detected in 286 control chromosomes, it was present in the paternal DNA.

Functional analysis of putative COL4A1 mutations

The first variant, COL4A1M1016V, is a mutation of a methionine residue in the Y position of the Gly-Xaa-Yaa repeat in the triple helix-forming domain. This amino acid is highly conserved and the analogous residue is a methionine in all vertebrate orthologues analyzed (Figure 8B) and in the COL4A1 paralogues COL4A3 and COL4A5 (data not shown). Moreover, there is precedence for a Y position methionine to valine mutation in COL4A5 causing Alport syndrome [44]. The second variant, COL4A1Q1316E, is a mutation of a glutamine in the Y position residue within the triple helix-forming domain. This glutamine residue is conserved in most vertebrates (Figure 8B). There is a strong preference for basic residues in the Y position of the Gly-Xaa-Yaa repeat and the mutation represents a substitution from a neutral amino acid to an acidic amino acid. Pathogenic mutations within the collagenous

domain often perturb triple helix assembly and mutations in regions of low thermal stability are predicted to be more disruptive

[45]. According to an algorithm that predicts collagen stability

[46], both mutations modestly reduce the thermal stability of their respective region (Figure 8C) although the biological relevance of this is equivocal.

Pathogenic mutations in the triple helix forming domain of several types of collagens impair secretion of the collagen heterotrimers and concomitantly, misfolded proteins accumulate within cells. To assess the functional significance of the COL4A1M1016V and COL4A1QJ316E mutations, we developed an assay to test the impact of the mutant proteins in a human cell line. We stably transfected HT1080 cells with wild-type or mutant COL4A1 cDNAs and determined the relative levels of intracellular and secreted COL4A1. To validate the assay, we tested the effect of the COL4A1G562E mutant allele that is established to cause familial small vessel disease in human patients [22,26]. As we predicted, when compared to HT1080 cells transfected with wildtype COL4A1, significant intracellular accumulation of COL4A1 and concurrent decrease in secreted COL4A1 was observed in cells transfected with the COL4A1G562E mutant cDNA (Figure 8D). The two putative pathogenic mutations were functionally tested using the same assay. Overall, the ratio of secreted/intracellular COL4A1 for the COL4A1M1016V mutation was reduced, however the results were variable (n = 9) and did not reach statistical significance. In contrast, and similar to the established CO-L4A1G562E mutation, the COL4A1(QJ316E mutation clearly impaired COL4A1 secretion leading to intracellular accumulation (p<0.001), supporting the hypothesis that the COL4A1Q1316E

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Figure 7. Col4a1+/Dex40 mice have functional, biochemical, histological, and genetically-complex myopathy. (A) Comparison of the peak grip force (mean +/—SEM) between Col4a1+/+ (blue; n = 7) and Col4a1+/Aex40 (red; n = 7) mice at 3 months of age revealed a significant reduction in Col4a1+/Aex40 mice (** indicates p<0.01). (B) Serum creatine kinase (CK) activity was compared (mean +/—SEM) between Col4a1+/+ (blue; n = 7) and Col4a1+/Aex40 (red; n = 7) mice before (Pre) and after (Post) exercise (* indicates p<0.05 vs all other groups). (C-E) Comparison of non-peripheral nuclei (arrows in D and E; mean +/—SEM in C) between Col4a1+/+ (counting of 17830 fibers in 5 muscles from 4 mice) and Col4a1+/Aex40 (counting of 13820 fibers in 4 muscles from 4 mice) mice (14 to 16 months old) revealed a significant increase in the number of non-peripheral nuclei in Col4a1+/Aex40 mice on the C57BL/6J background (control mean = 1.5%; mutant mean = 5.2%; p<0.0005). This difference is modified by the genetic context. There was no difference between Col4a1+/+ and Col4a1+/Aex40 CASTB6F1 mice and there was a significant rescue of non-peripheral nuclei in CAST/B6F1 Col4a1+/Aex40 mice compared to C57BL/6J Col4a1+/Aex40 mice (p<0.005). Scale bar: 100 mm. doi:10.1371/journal.pgen.1002062.g007

mutation is pathogenic. Importantly, the accumulation of both the monomeric and heterotrimeric forms of COL4A1 suggests that the COL4A1G562E and COL4A1Q1316E mutations impaired triple helix assembly and/or stability.

The mechanism of Col4a1 -induced disease is independent of dystroglycan glycosylation

Biochemical analyses revealed that known MEB/WWS-causing mutations act via hypoglycosylation of dystroglycan [11]. Although COL4A1 is not directly involved in post-translational dystroglycan modification, misfolded COL4A1Dex40 proteins in the endoplasmic reticulum (ER) might indirectly impair dystroglycan post-translational modification. Similarly, ER stress can produce reactive oxygen species [47-50] and exposure to reactive oxygen species can result in dystroglycan de-glycosylation [51]. Thus, Col4a1-induced pathogenesis might still act indirectly via dystroglycan hypoglycosylation or de-glycosylation. To test for alterations in dystroglycan expression and/or post-translational processing, we performed Western blot analysis on wheat germ agglutinin (WGA)-enriched skeletal muscle extracts and compared the amount and mobility of a- and b- dystroglycan between Col4a1+/+ and Col4a1+/A'x40 mice using a polyclonal b-dystrogly-

can antibody and the IIH6 a-dystroglycan antibody that recognizes the fully glycosylated, functional form of a-dystroglycan [11]. Immunoreactivity to, and mobility of, the precursor dystroglycan protein (WGA-unbound fraction) and a- and b-dystroglycan (WGA-bound, glycoprotein enriched fraction) were indistinguishable between Col4a1+/+ and Col4a1+/A'x40 mice (Figure 9). Consistent with this finding, immunolabeling of muscle sections for a- and b- dystroglycan showed similar expression patterns in the sarcolemma membrane of Col4a1+/+ and Col4a1+/A'x40 mice. These data indicate that dystroglycan expression, localization and post-translational modification are not altered in Col4a1+/A'x40 mice and that myopathy arises via disruption of the basal lamina.


In this study, we show that COL4A1 mutations cause multiple pathophysiological hallmarks of MEB/WWS in mice and possibly MEB/WWS in human patients. Mice harboring a semi-dominant Col4a1 mutation have ocular dysgenesis, cerebral cortical lamination defects and myopathy. Optic nerve hypoplasia results, at least in part, from mislocalization and increased apoptosis of RGCs during development. Cerebral cortical malformations range from

Figure 8. Identification of COL4A1 missense mutations in two human patients. Direct sequence analysis of patient DNA revealed two heterozygous missense mutations (A, top panels) that were not found in control patients (A, bottom panels). (B) Protein sequence alignments with multiple species indicate a very strong degree of conservation of the altered amino acid for both mutations. Methionine at position 1016 is conserved in all species tested except for C. elegans and Drosophila where this amino acid is a lysine. Glutamine at position 1316 is also highly conserved and is never a glutamate residue. (C) Triple helix thermal stability was calculated by amino acid sequence and is plotted for the length of the COL4A1 protein. Arrows indicate the positions for each mutation and the effects of the mutations on calculated thermal stability are indicated in red. (D) For quantitative analysis of the ratio of secreted to intracellular mutant COL4A1 protein levels, values are expressed as percentage of the wild-type COL4A1 ratio and are presented as mean +/— SEM. Representative Western blot images for secreted and intracellular COL4A1 are shown below the graph. Nine independent Western blot experiments were performed using 6 independent clones per mutation for this analysis, **P<0.01. doi:10.1371/journal.pgen.1002062.g008

(tl № № ftJ

2 3 2 S

kDa -206

Figure 9. The pathogenic mechanism by which Col4a1 mutation causes MEB/WWS phenotypes is independent of dystroglycan glycosylation. (A) Western blot analysis of total protein lysates from quadriceps biopsy using antibody for dystroglycan precursor protein (bottom) did not reveal a difference in dystroglycan precursor expression between Col4a1+/+ and Col4a1+/Dex40 mice. To test for differences in quantity or mobility (glycosylation) of a-dystroglycan and b-dystroglycan, lysates were enriched for glycosylated proteins by WGA binding. WGA-enriched fraction of quadriceps protein extracts did not reveal differences in quantity or glycosylation of either a-dystroglycan (top) or b-dystroglycan (middle) between Col4a1+/+ and Col4a1+/Dex40 mice. (B) Immunohistochemical labeling of muscle sections with antibodies against the glycosylated form of a-dystroglycan (red) and b-dystroglycan (green) show similar patterns of a- and b-dystroglycan expression between Col4a1+/+and Col4a1+/Dex40 mice. doi:10.1371/journal.pgen.1002062.g009

subtle lamination defects to large structural abnormalities. Myopathy is mild, but consistent and significant, and is exacerbated by exercise but not by age. Importantly, myopathy was genetic background-dependent, which implies that in resistant genetic contexts Col4a1- induced myopathy might not be detected but that on permissive genetic backgrounds, myopathy could be severe. Two out of 27 MEB/WWS patients tested had COL4A1 mutations and in at least one case the mutation interfered with protein secretion. Moreover, unlike all other known causes of MEB/WWS, the cellular mechanism underlying Col4a1-induced pathology is independent of dystroglycan post-translational modification.

Until now, the precise pathogenic mechanism resulting from COL4A1 mutations in human patients was poorly characterized and was only presumed to impair protein secretion and cause

intracellular accumulation. Here, we have established and validated a cellular assay that demonstrates this effect. However, the relative contribution of COL4A1 deficiency in the basement membrane versus toxic intracellular accumulation to pathogenesis is difficult to dissociate and other possible mechanisms exist. Although the ratio of secreted to intracellular protein for the COL4A1M1016V mutation was not statistically different from wildtype there was an overall reduction in the ratio. It is important to bear in mind that absence of statistical significance does not necessarily imply absence of biological relevance. For instance, more efficient intracellular degradation of some misfolded proteins could prevent detection of an altered ratio of secreted to intracellular COL4A1 for some mutations. Alternatively, these data could indicate a third pathogenic mechanism whereby mutant proteins are secreted and exert a detrimental extracellular effect. In support of such a mechanism, the COL4A1M1016V mutation occurs within a putative binding site for the matricellular glycoprotein SPARC (secreted protein acid and rich in cysteine) [52] and could therefore act by disrupting protein-protein interaction in the extracellular matrix.

These variants represent the first heterozygous mutations in MEB/WWS patients. Although generally considered to be recessive, the inheritance pattern in many MEB/WWS patients is unknown. Phenotypic variability, reduced penetrance, and de novo mutations can all explain how dominant mutations might appear recessive in small families. Here, we observed non-penetrance of the COL4A1Q1316E mutation in the father of the affected child. Families with COL4A1 mutations are only now starting to be identified but apparent non-penetrance, and asymptomatic carriers have already been reported [29,53]. Importantly, Col4a1-associated phenotypes in mice are not only influenced by environmental factors but are also genetic context-dependent, and reduced penetrance could reflect modifier loci. Alternatively, genetic mosaicism of dominant collagen mutations can explain asymptomatic carriers [54].

We identified mutations in two out of 27 CMD patients tested. DNA was limiting and prevented us from also sequencing COL4A2; however, evidence from mice and C. elegans support that mutations in COL4A2 may cause phenotypes similar to those resulting from mutations in COL4A1 [24,45,55]. It will be very important in the future to determine whether the COL4A1 findings extend to COL4A2. This could have an even broader relevance as COL4A1 mutations are pleiotropic with variable penetrance and expressivity from organ to organ. It is possible that COL4A1 mutations underlie CMD in patients with different, non-MEB/ WWS-like, subclasses of the disease. Based on our current data, the most likely patients are those with mild myopathy and those with clinical manifestations that overlap with some of the other COL4A1-related phenotypes described in non-MEB/WWS syndromes including porencephaly, renal disease and cerebrovascular disease. Collectively, these findings provide the impetus for COL4A1 and COL4A2 mutation analysis in further cohorts of patients with CMD and/or congenital cerebral malformations.

COL4A1 mutations have been identified in families with a spectrum of diseases affecting the cerebral vasculature, although pathologies of the eyes, kidneys and muscles have also been reported [21,22,25-29,31,33,56,57]. Notably, COL4A1 appears to be an important genetic cause of porencephaly - a condition usually diagnosed in infants and characterized by large cerebral cystic cavities that communicate with the ventricles. Importantly, the cortical malformations present in MEB/WWS and described in the current manuscript are clinically and mechanistically distinct from porencephalic cavities, which are predicted to result from pre- or peri-natal hemorrhages in the germinal matrix.

Col4a1 mutations are pleiotropic and our findings expand the phenotypic spectrum resulting from Col4a1 mutations in mice. We propose that COL4A1 mutations in human patients will reflect the pleiotropy observed in mice and will be involved in the pathogenesis of diseases clinically distinct from those reported previously. Optic nerve hypoplasia and cortical neuronal migration defects have not been described in human patients with COL4A1 mutations and a role for COL4A1 or COL4A2 in MEB/ WWS-like diseases was not previously suspected. Interestingly, six families with COL4A1 mutations and cerebrovascular disease also had muscle cramps and CK elevation [28,33]. Although the degree of CK elevation in Col4a1 mutant mice is less than that observed in dystrophin-glycoprotein complex related disorders, it is comparable to that seen in other types of dystrophies involving extracellular matrix proteins, including collagen VI -associated Bethlem myopathy and Ullrich CMD. While the exact mechanism underlying myopathy in collagen VI-related disorders is unclear, several lines of evidence suggest that mitochondrial dysfunction may be an important player [58].

We show that genetic context is an important factor contributing to the variable penetrance and severity of Col4a1-related diseases and that the CAST/EJ strain can modify myopathy. These findings also imply that certain genetic contexts might exacerbate myopathy and that COL4A1 mutations could also cause severe CMD. Importantly, genetic modifiers can be general or tissue specific and tissue-specific modification could help explain how mutations in a single gene can contribute to such diverse phenotypes. We also demonstrate that gene-environment interactions contribute to phenotypic variability. We have previously reported that cesarean delivery can reduce the risk of perinatal intracerebral hemorrhages and here we show that elevation in CK activity is exercise-dependent. Allelic differences could also help explain variable expressivity and severity of COL4A1-associated diseases between patients. For instance, a mutation that affects heterotrimer assembly might have broader effects than a mutation that specifically affects interactions with cell surface receptors, growth factors or other extracellular matrix molecules. Interestingly allelic differences are already emerging pointing to genotype/phenotype correlations in some families [28,33].

Pial basement membrane integrity is critical for normal cortical development and insufficient COL4A1 in basement membranes renders them prone to disruption. Breaches in the pial membrane cause alterations in cortical neuronal distribution [17,59,60]. These are not intrinsic neuronal migration defects but are secondary to disorganization of the cortical marginal zone, and to defects in anchorage of glial endfeet and formation of the Cajal-Retzius cell layer [16]. Reactive gliosis and increased GFAP labeling may reflect broader breaches of the glia limitans including the blood brain barrier [11,42]. Thus, while some structural defects might be due to disruptions of the pial basement membrane, other pathology might be attributed to disruptions of vascular basement membranes and/or defects in blood brain barrier function.

Understanding the precise mechanism underlying myopathy is complicated by the presence of COL4A1 in basement membranes of the sarcolemma, myotendonous junctions and neuromuscular junctions. Notably, Col4a1 mutant mice have transient neuromus-cular junction abnormalities that reportedly resolve by 3 weeks of age [61]. Determination of the primary site of pathogenesis for myopathy will likely require conditional expression of the Col4a1 mutation. However, evidence from other model systems suggests that the primary mechanism is load-induced muscle fiber detachment. For instance, the most common form of CMD

(MDC1A) is caused by mutations in the basement membrane component laminin alpha 2 (LAMA2) [62] and in zebrafish with mutations in the LAMA2 ortholog, muscle contractions lead to muscle fiber detachment and loss [63]. This mechanism is consistent with exercise-induced CK elevation in Col4a1 mutant mice. This mechanism is also consistent with the observation that C. elegans Col4a1 mutants die with ruptured basement membranes shortly after muscle contractions begin [45,55]. A similar mechanism in alveolar basement membranes could also explain why newborn Col4a1 mutant mice have respiratory distress immediately after starting to breathe [22].

Thus, myopathy resulting from mutations in basement membrane components LAMA2 and COL4A1 might share a common pathogenic mechanism whereby contraction-induced load leads to muscle fiber detachment. Given this potential shared mechanism and the phenotypic variability of COL4A1 mutations, we propose that MDC1A patients that are negative for LAMA2 mutations are suitable candidates for screening for mutations in COL4A1 and COL4A2. Importantly, if a component of the pathology is secondary to deleterious consequences of compromised blood brain barrier and load-induced myopathy, there is the potential for therapeutic interventions to blunt the severity of this devastating disease. For example, conditions that promoted protein folding and increased COL4A1 secretion in C. elegans mutants were able to rescue muscle contraction-induced basement membrane disruptions and promoted viability and survival [55]. Thus, chemical chaperones, or other methods to promote protein folding, might have therapeutic potential in human patients and improve the prognosis for MEB/WWS patients.

Materials and Methods

Ethics statement

Animals. Experiments were conducted in compliance with protocols approved by the Institutional Animal Care and Use Committee at University of California in San Francisco.

Human patients. Written, informed consent was obtained from the subjects or their legal guardians. The Institutional Review Boards of Beth Israel Deaconess Medical Center and Children's Hospital Boston and the UCSF Committee on Human Research approved this study.

Retinal analyses

Heads were equilibrated in 20% sucrose in phosphate buffered saline (PBS) overnight at 4°C, embedded in OCT compound (Sakura Finetek), and flash frozen using dry ice/ethanol. For each genotype we analyzed six eyes at E14 and E16 and eighteen eyes at E18. For each eye, at least 3 position-matched, transverse sections (20 mm) were co-labelled with antibodies against Islet-1 (1:10, Developmental Studies Hybridoma Bank 39.4D5sup and laminin (1:1000, Abcam), or Islet-1 and activated Caspase-3 (1:1000, R&D Systems). Sections were blocked (Tris-buffered saline with 0.1% Triton-X (TBST) containing 5% normal goat serum (Invitrogen)) for 1 hour at room temperature (RT) and washed twice for 2 min with TBS. Labeling was performed in TBS containing 3% BSA (Sigma) overnight at 4°C. Sections were incubated with Alexa Fluor 488 goat anti-mouse and Alexa Fluor 594 goat anti-rabbit secondary antibodies (1:1000, Invitrogen) for 2 hours at RT and cover-slipped with Vectashield Hard Set with DAPI (Vector Labs).

Cerebral analyses

Adult brains were fixed by trans-cardiac perfusion with 4% paraformaldehyde (PFA) in 0.1 M sodium phosphate, pH 7.4 then

post-fixed in PFA at 4°C overnight and prepared for cryosection-ing as described above. For Nissl staining, coronal sections (25 mm) were hydrated for 5 min in buffer (0.2% sodium acetate, 0.3% glacial acetic acid) then incubated for 10 min in Nissl stain (0.02% cresyl violet in buffer). Sections were washed in water (2 x2 min) and de-stained for 15 sec (0.3% glacial acetic acid in 70% ethanol) before being dehydrated, cleared in xylene and cover-slipped with Permount (Fisher).

For GFAP and NeuN immunostaining, sections were incubated in 1% H202 in PBS for 15 min, washed in PBS, blocked in PBS containing 5% NGS, 0.1% Triton-X, and 1% BSA in PBS for 1 hour at RT. Sections were incubated with anti-GFAP antibody (1:500, Chemicon) or anti-NeuN antibody (Chemicon 1:500) overnight at 4°C, washed in PBS containing 0.1%Triton-X, and incubated for 2 hr with biotinylated goat anti-rabbit or goat anti-mouse antibody (1:500, Vector Labs). Sections were then incubated in avidin-biotin solution (Vector Labs) for 90 min and immunoreactivity was visualized by treating sections with diaminobenzidine (DAB, Vector Labs). Sections were dehydrated, cleared in xylene and cover-slipped with Permount (Fisher).

For BrdU labeling, pregnant mice were injected with BrdU (50 mg/kg) at gestational days 14, or 16. At P0, heads were fixed in 4% PFA for 4 hr, embedded in OCT, snap frozen in dry ice/ ethanol bath. Cryosections (25 mm) were incubated in sodium citrate buffer (10 mM sodium citrate, pH 6.0, 0.05% Tween-20) for 30 min starting at 90°C and allowed to cool, rinsed in water, incubated in 1 M HCl containing 0.2 mg/ml pepsin for 10 min at RT, in 2 N HCl for 20 min at 37°C. Sections were then washed and labeled with anti-BrdU (1:30, Accurate Chemical) and biotinylated goat anti-rat secondary (1:500, Vector Labs). For laminin immunolabeling of P0 brains, heads were processed as described in the retinal analyses section and 20 mm coronal sections were immunolabeled with anti- laminin antibody (1:1000, Abcam).

Muscle analyses

Peak grip force was determined using a Grip Strength Meter (Columbus Instruments) and using the average from 3 consecutive trials on each animal. For CK measurements, blood was drawn before and after exercise from the tail vein in a hematocrit tube, centrifuged for 5 min, and the serum was collected. CK activity was measured using CK-NADP assay (Raichem) and a microplate reader (Biorad). Exercise was 30 minutes with a 15° downhill grade on a treadmill equipped with a shock plate (Columbus Instruments). Animals were started at 7 m/min and increased 3 m/min every 2 min until maximum of 16 m/min speed was reached. Non-peripheral nuclei were evaluated in quadriceps and tibialis anterior muscles dissected and frozen in liquid nitrogen-cooled isopentane. Cryosections (8 mm) were stained with hematoxylin and eosin for histopathology or labeled with anti-pan laminin antibody (Sigma), followed by an anti-rabbit AlexaFluor-488 secondary antibody, and the nuclei were labeled with DAPI. The observers were masked to genotypes and counted between 2000-5000 fibers per animal.

For dystroglycan enrichment and immunoblotting, quadriceps muscle biopsy (100 mg) was homogenized in TBS (pH 7.5) containing 1% Triton-X and protease inhibitors (Thermo Scientific Pierce). The soluble fraction was incubated overnight at 4°C with 200 ul of wheat germ agglutinin (WGA)-agarose beads (Vector Laboratories) to enrich for glycosylated proteins. Beads were washed three times with 1 ml TBS containing 0.1%Triton-X and protease inhibitors (Thermo Fisher) and were boiled in SDS-PAGE loading buffer for 5 minutes. Proteins (50 ml) were separated on 10% SDS-PAGE and transferred to polyvinylidene

fluoride (PVDF) membranes (BioRad). Membranes were blocked for 2 hours at room temperature in 5% non-fat milk in TBS containing 0.1% Tween-20, incubated overnight with a mouse monoclonal anti- a-dystroglycan antibody recognizing the glycosylated form of a-dystroglycan (IIH6; 1:1000, Millipore), or rabbit polyclonal anti- b-dystroglycan antibody (raised against the C-terminus of dystroglycan precursor; 1:400, Santa Cruz) diluted in blocking buffer. Membranes were washed in TBS containing 0.1% Tween-20, incubated 2 hours at room temperature with horseradish peroxidase-conjugated secondary antibody raised in donkey (anti-mouse IgM, and anti-rabbit IgG, respectively; 1:10 000, Jackson Immunoresearch) diluted in blocking buffer. Immunore-activity was visualized using chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate, Thermo Scientific).

For immunostaining, muscle cryosections (25 mm) were incubated with either b-dystroglycan (1:150) or with a-dystroglycan (1:400) antibody diluted in blocking buffer (10% normal donkey serum, 0.2% BSA in PBS containing 0.1% Triton-X) overnight at 4°C and incubated with Alexa Fluor 488 conjugated donkey anti-rabbit (Invitrogen) or cy5- conjugated donkey anti-mouse IgM (Jackson Immunoresearch).

For COL4A1 immunolabeling, muscle cryosections (25 mm) were fixed in acetone for 10 min, rinsed in TT buffer (50 mM Tric-HCl, pH 7.4, 0.1% Tween-20), incubated in acid solution (0.1 M KCl/HCl, pH 1.5), washed three times in TT buffer and incubated for an hour in blocking buffer (10% normal donkey serum and 2 mg/ml BSA in Tris buffer). Sections were then incubated with rat anti- COL4A1 (H11) monoclonal antibody (1:200, Shigei Medical Research Institute, Japan) diluted in TT buffer overnight at 4°C and for 1 hour with Alexa Fluor 488 conjugated donkey anti-rat secondary antibody (Invitrogen) and cover-slipped using mowiol mounting medium (Calbiochem).


Images were captured using an AxioImager M1 microscope equipped with an AxioCam MRm digital camera for fluorescence or AxioCam ICc3 for brightfield and AxioVision software (Zeiss).

Human analyses

Genomic DNA (10 ng/mL) sequencing was performed using ABI BigDye v3.1 and analyzed using Sequencher software (Gene Codes Corporation). Control samples are from ethnically matched adults with no history of neurological disease.

Functional analysis of human mutations

HT1080 Human fibrosarcoma cells were transfected using Superfect reagent (Qiagen) with the expression vector pReceiver-M02 vector (GeneCopoeia) containing a CMV promoter upstream of GFP (to evaluate transfection efficiency), wild-type Col4a1 or mutant Col4a1 cDNA cloned and a neomycin resistance cassette, allowing stably transfected cells to be selected using G418 (Invitrogen). After 12 days of G418 selection (600 mg/ml), individual surviving clones were isolated and expanded in presence of 600 mg/ml of G148.

Stably transfected HT1080 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with penicillin, streptomycin, nonessential amino acids, glutamine, sodium pyruvate, G418 (250 mg/ml for maintenance), 10% Fetal Bovine Serum (FBS) and ascorbic acid (50 mg/ml) at 37°C in 5% CO2 in a humid atmosphere until they reach 80-90% confluence. Then, cells were serum-deprived for 24 hours under the same culture conditions and harvested and protein were extracted from HT1080 cells using cell extraction buffer containing 0.05 M Tris-HCl pH 8.0, 0.15 M NaCl, 5.0 mM EDTA, 1% NP-40, and

protease inhibitors (Pierce) at 4°C. After centrifugation (13000 rpm, 20 min at 4°C), the soluble fraction of the whole cell lysate was collected for subsequent Western blot analysis. The conditioned medium was collected at the same time and supplemented with protease inhibitors.

Determination of protein concentration in the soluble fraction was performed using a DC protein assay (BioRad) and 45 ug of total protein were separated on a 4-15% gradient SDS-PAGE under non-reducing conditions and transferred to polyvinylidene fluoride (PVDF) membranes (BioRad). For the conditioned medium, the volume loaded on the gel was adjusted based on the protein concentration of the soluble fraction, which is assumed to reflect the number of cells for a given sample. Membranes were blocked for 2 hours at room temperature in 5% non-fat milk in TBS containing 0.1% Tween-20, and overnight at 4°C in 3% BSA in TBS. Membranes were then incubated with rat anti- COL4A1 (H11) monoclonal antibody (1:100, Shigei Medical Research Institute, Japan) in 1% BSA in TBS for 3 hours at room temperature and were washed in TBS containing 0.1% Tween-20, incubated 2 hours at room temperature with horseradish peroxidase-conjugated secondary antibody raised in donkey (anti-Rat IgG 1:10 000, Jackson Immunoresearch) diluted in 5% non-fat milk in TBS containing 0.1% Tween-20. Immunoreactivity was visualized using chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate, Thermo Scientific). Densitometric analysis was performed on low exposure images using the NIH Image J software (National Institutes of Health). For quantitative analysis of the ratio of secreted to intracellular mutant COL4A1 protein, the amount of COL4A1 detected in the conditioned medium was divided by the amount of intracellular COL4A1 normalized with actin.

Statistical analyses

In experiments where two groups were compared, samples were compared by a Student's T-test with p<0.05 considered significant. In experiments where more than two groups were compared, samples were analyzed by one-way ANOVA followed by a Tukey's post hoc test with p<0.05 considered significant. For calculation of apoptotic index, one E18 Col4a1+/Aex40 eye had an extremely high apoptotic index (12.9) that was considered an outlier by Grubbs' test (p<0.01 vs. all mutants and Z = 2.93248; vs. all samples Z = 3.26997) and was removed from subsequent statistical analyses.

Supporting Information

Figure S1 Col4a1+/A'x40 mice have hippocampal neuronal localization defects. Representative cresyl violet stained coronal sections from adult Col4a1+/+ (A-D) and position-matched Col4a1+/A'x40 (E-H) brains revealed subtle but consistent neuronal localization defects in hippocampi of mutant mice. Regions of the CA1 (red boxes in A and E), CA3 (black boxes in A and E) and dentate gyrus (white boxes in A and E) are enlarged in B and F, C and G, and D and H, respectively. All mutant mice (n = 6) had hippocampal defects that included focal distortions (red box in E, compared to A) as well as diffusion of pyramidal cell layers in the CA1 (F, compared to B) and CA3 (G, compared to C) regions and granular cell layers in the dentate gyrus (H, compared to D). Scale bars: A and E, 500 mm; B-D and F-H, 100 mm. (TIF)

magnification images revealed increased labeling in Col4a1+ ex mice (E compared to B) and the astrocytic morphology of the labeled cells. Increased labeling was also detected in Col4a1+/A'x40 hippocampi compared to Col4a1+/+ (F compared to C). Scale bars: A, C, D, F 500 mm; B and E, 100 mm. (TIF)

Figure S3 COL4A1 is present in skeletal muscle basement membrane of Col4a1+/+ and Col4a1+/Aex40 mice. Immunohisto-chemical labeling of muscle sections with antibodies against COL4A1 confirmed that COL4A1 is present in Col4a1+/+ (A) and Col4a1+/A'x40 (B) skeletal muscle basement membrane and revealed that the mutant basement membrane appeared to label less uniformly and less intensely. (TIF)

Figure S4 Brain imaging of patients 1 and 2. Brain magnetic resonance imaging in patient 1 (A-B), a normal control (C-E) and patient 2 (F-G). Patient 1 has a typical WWS phenotype with a severe cobblestone-type cortical malformation with a smooth brain surface that resembles lissencephaly, a thin and discontinuous laminar heterotopia just below the cortex (arrow in A), diffusely abnormal white matter (asterisk in A), thin brainstem, enlarged tectum (arrowhead in B), moderate kink at the midbrain-pons junction (arrow in B), severe cerebellar hypoplasia (seen in both A and B), and enlarged posterior fossa. The cortex is ~10 mm thick with an irregular gray-white border (A), while classic lissencephaly is typically 12-20 mm thick with a smooth gray-white border. The brainstem kink is less severe than typical for WWS. Patient 2 has a less severe MEB phenotype with diffuse cobblestone-type cortical malformation that appears thicker over the frontal lobes (best seen in G), diffuse abnormal white matter signal (asterisk in G), moderately enlarged lateral ventricles (F and G) and thin brainstem with flat pons (arrow in F). The gyral pattern is irregular and resembles polymicrogyria, but no actual microgyri are seen. These are patients LP93-014 and LP90-029 from the Dobyns database. Parts A and B are modified from Figure 1 in [Kanoff, et al]. [Kanoff RJ, Curless RG, Petito C, Falcone S, Siatkowski RM, et al., (1998) Walker-Warburg syndrome: neurologic features and muscle membrane structure. Pediatr Neurol 18:76-80.]. (TIF)

Table S1 Sequencing primers for COL4A1. (PDF)

Table S2 Non-Synonymous coding variants identified. (PDF)

Table S3 Synonymous coding variants identified. (PDF)

Table S4 Intronic variants identified. (PDF)

Text S1 Clinical details of human patients. (DOC)


We thank Drs. Samuel Pleasure and Anthony Wynshaw-Boris for critical comments on the manuscript and Dr. James Barkovich for helpful discussions.

Figure S2 Presence of reactive astrocytes in the brain of Author Contributions


mice. Representative coronal brain sections from adult Col4a1+/+ (A-C) and position matched Col4a1+/A'x40 (D-F) mice labeled with anti-GFAP antibody revealed the presence of reactive astrocytes. Compared to Col4a1+/+ brains (A), Col4a1+/A'x40 mice had increased GFAP labeling in the cortex (D). Higher

Conceived and designed the experiments: DBG. Performed the experiments: CL-D DJD EPH MdL DL ZK. Analyzed the data: CL-D DJD EPH MdL DL ZK MCM WBD CAW DEM DBG. Contributed reagents/ materials/analysis tools: MCM WBD CAW DEM DBG. Wrote the paper: CL-D DBG.


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